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Information on EC 1.1.1.390 - sulfoquinovose 1-dehydrogenase and Organism(s) Pseudomonas putida and UniProt Accession P0DOV5

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IUBMB Comments
The enzyme, characterized from the bacterium Pseudomonas putida SQ1, participates in a sulfoquinovose degradation pathway. Activity with NADP+ is only 4% of that with NAD+.
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This record set is specific for:
Pseudomonas putida
UNIPROT: P0DOV5
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The taxonomic range for the selected organisms is: Pseudomonas putida
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
sq dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SQ-dehydrogenase
-
SQ dehydrogenase
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
6-deoxy-6-sulfo-D-glucopyranose:NAD+ 1-oxidoreductase
The enzyme, characterized from the bacterium Pseudomonas putida SQ1, participates in a sulfoquinovose degradation pathway. Activity with NADP+ is only 4% of that with NAD+.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sulfoquinovose + NAD+
6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
show the reaction diagram
-
-
-
r
sulfoquinovose + NAD+
6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
show the reaction diagram
-
sulfoquinovose is 6-deoxy-6-sulfoglucose
-
-
?
sulfoquinovose + NADP+
6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
show the reaction diagram
-
when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+
-
-
?
additional information
?
-
-
the enzyme shows no activity with D-glucose-6-phosphate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sulfoquinovose + NAD+
6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
show the reaction diagram
-
-
-
r
sulfoquinovose + NAD+
6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
show the reaction diagram
-
sulfoquinovose is 6-deoxy-6-sulfoglucose
-
-
?
additional information
?
-
-
the enzyme shows no activity with D-glucose-6-phosphate
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
dependent on
NADP+
-
when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 2.4
sulfoquinovose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4 - 33.8
sulfoquinovose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6 - 67.6
sulfoquinovose
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
DSM 100120
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
strain SQ1 shows the ability to utilize the plant sugar sulfoquinovose (6-deoxy-6-sulfoglucose) for growth
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the sulfoquinovose, SQ, degradation pathway, that is distinct from the entner-Doudoroff pathway and the pathway in Escherichia coli, the pathway operative in Puitida putida strain SQ1 represents a second, yet unknown bacterial degradation pathway for SQ, overview
physiological function
the enzyme is involved in the degradation of sugar sulfoquinovose, SQ, the polar headgroup of the plant sulfolipid sulfoquinovosyl diacylglycerol, which is present in the photosynthetic membranes of all higher plants, mosses, ferns and algae and most photosynthetic bacteria
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SQD_PSEPU
260
0
27870
Swiss-Prot
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
genomic analysis, DNA and amino acid sequence determination and analysis
expressed in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Felux A.-K, F.A.; Spiteller D, S.D.; Klebensberger J, K.J.; Schleheck D, S.D.
Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1
Proc. Natl. Acad. Sci. USA
112
E4298-E4305
2015
Pseudomonas putida, Pseudomonas putida SQ1
Manually annotated by BRENDA team
Felux A.-K, F.A.; Franchini P, F.P.; Schleheck D, S.D.
Permanent draft genome sequence of sulfoquinovose-degrading Pseudomonas putida strain SQ1
Stand. Genomic Sci.
10
42-47
2015
Pseudomonas putida (P0DOV5), Pseudomonas putida SQ1 (P0DOV5)
Manually annotated by BRENDA team