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Information on EC 1.1.1.383 - ketol-acid reductoisomerase [NAD(P)+] and Organism(s) Shewanella sp. and UniProt Accession A0KS29

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IUBMB Comments
The enzyme, characterized from the bacteria Hydrogenobaculum sp. and Syntrophomonas wolfei subsp. wolfei and from the archaea Metallosphaera sedula and Ignisphaera aggregans, can use both NADH and NADPH with similar efficiency [cf. EC 1.1.1.86, ketol-acid reductoisomerase (NADP+) and EC 1.1.1.382, ketol-acid reductoisomerase (NAD+)].
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This record set is specific for:
Shewanella sp.
UNIPROT: A0KS29
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The taxonomic range for the selected organisms is: Shewanella sp.
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms
ilvC, Shewana3_0355, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Shewana3_0355
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
(2R)-2,3-dihydroxy-3-methylbutanoate:NAD(P)+ oxidoreductase (isomerizing)
The enzyme, characterized from the bacteria Hydrogenobaculum sp. and Syntrophomonas wolfei subsp. wolfei and from the archaea Metallosphaera sedula and Ignisphaera aggregans, can use both NADH and NADPH with similar efficiency [cf. EC 1.1.1.86, ketol-acid reductoisomerase (NADP+) and EC 1.1.1.382, ketol-acid reductoisomerase (NAD+)].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+
show the reaction diagram
-
-
-
?
(S)-2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.075 - 0.415
NADH
0.001 - 1
NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 2.4
NADH
0.1 - 4.5
NADPH
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.6 - 32
NADH
0.17 - 4500
NADPH
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A71S/R76D/S78AD/Q110V
mutant engineered for reversal in cofactor specificity for NADH over NADPH
R76D/Ser78D
mutant engineered for reversal in cofactor specificity for NADH over NADPH
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Brinkmann-Chen, S.; Flock, T.; Cahn, J.; Snow, C.; Brustad, E.; McIntosh, J.; Meinhold, P.; Zhang, L.; Arnold, F.
General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH
Proc. Natl. Acad. Sci. USA
110
10946-10951
2013
Escherichia coli (P05793), Lactococcus lactis subsp. lactis (Q02138), Lactococcus lactis subsp. lactis IL1403 (Q02138), Shewanella sp. (A0KS29), Shewanella sp. ANA-3 (A0KS29), Slackia exigua (D0WGK0), Slackia exigua DSM 15923 (D0WGK0)
Manually annotated by BRENDA team