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Information on EC 1.1.1.371 - scyllo-inositol 2-dehydrogenase (NADP+) and Organism(s) Bacillus subtilis and UniProt Accession O32223

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IUBMB Comments
The enzyme, found in the bacterium Bacillus subtilis, has no activity with NAD+ [cf. EC 1.1.1.370, scyllo-inositol 2-dehydrogenase (NAD+)].
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This record set is specific for:
Bacillus subtilis
UNIPROT: O32223
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The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
iolU, IolW, NADP+-dependent SI dehydrogenase, yulF, yvaA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NADP+-dependent SI dehydrogenase
-
yvaA
formerly, gene name
NADP+-dependent SI dehydrogenase
-
SYSTEMATIC NAME
IUBMB Comments
scyllo-inositol:NADP+ 2-oxidoreductase
The enzyme, found in the bacterium Bacillus subtilis, has no activity with NAD+ [cf. EC 1.1.1.370, scyllo-inositol 2-dehydrogenase (NAD+)].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
scyllo-inositol + NADP+
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
show the reaction diagram
scyllo-inositol + NADP+
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
scyllo-inositol + NADP+
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
show the reaction diagram
-
-
-
?
scyllo-inositol + NADP+
2,4,6/3,5-pentahydroxycyclohexanone + NADPH + H+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.68
2,4,6/3,5-pentahydroxycyclohexanone
pH 7.5, 37°C
0.096
NADP+
pH 7.5, 37°C
0.027
NADPH
pH 7.5, 37°C
34.3
scyllo-Inositol
pH 7.5, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
IolW shares a significant similarity with YulF, another NADP+-dependent SI dehydrogenase
evolution
YulF shares a significant similarity with IolW, another NADP+-dependent SI dehydrogenase
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a C-terminally His6-tagged fusion protein
gene iolW, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family
gene yulF, DNA and amino acid sequence determination and analysis, the gene belongs to the Gfo/Idh/MocA family, recombinant expression of His6-tagged YulF
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
induced during growth on scyllo-inositol
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Morinaga, T.; Ashida, H.; Yoshida, K.
Identification of two scyllo-inositol dehydrogenases in Bacillus subtilis
Microbiology
156
1538-1546
2010
Bacillus subtilis (O32223), Bacillus subtilis 168 (O32223)
Manually annotated by BRENDA team
Kang, D.M.; Tanaka, K.; Takenaka, S.; Ishikawa, S.; Yoshida, K.I.
Bacillus subtilis iolU encodes an additional NADP(+)-dependent scyllo-inositol dehydrogenase
Biosci. Biotechnol. Biochem.
81
1026-1032
2017
Bacillus subtilis (O05265), Bacillus subtilis (O32223), Bacillus subtilis 168 (O05265), Bacillus subtilis 168 (O32223)
Manually annotated by BRENDA team