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Information on EC 1.1.1.370 - scyllo-inositol 2-dehydrogenase (NAD+) and Organism(s) Bacillus subtilis and UniProt Accession P40332

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IUBMB Comments
The enzyme, found in the bacterium Bacillus subtilis, has no activity with NADP+ [cf. EC 1.1.1.371, scyllo-inositol 2-dehydrogenase (NADP+)]. It is part of a scyllo-inositol degradation pathway leading to acetyl-CoA.
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Bacillus subtilis
UNIPROT: P40332
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The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
scyllo-inositol dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NAD+-dependent SI dehydrogenase
-
yisS
formeryl, gene name
SYSTEMATIC NAME
IUBMB Comments
scyllo-inositol:NAD+ 2-oxidoreductase
The enzyme, found in the bacterium Bacillus subtilis, has no activity with NADP+ [cf. EC 1.1.1.371, scyllo-inositol 2-dehydrogenase (NADP+)]. It is part of a scyllo-inositol degradation pathway leading to acetyl-CoA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
scyllo-inositol + NAD+
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
show the reaction diagram
scyllo-inositol + NAD+
scyllo-inosose + NADH + H+
show the reaction diagram
-
-
-
r
scyllo-inosose + NADH + H+
scyllo-inositol + NAD+
show the reaction diagram
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
scyllo-inositol + NAD+
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
no activity with NADP+
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.85
2,4,6/3,5-pentahydroxycyclohexanone
pH 7.5, 37°C
0.15
NAD+
pH 7.5, 37°C
0.019
NADH
pH 7.5, 37°C
17.1
scyllo-Inositol
pH 7.5, 37°C
2.85
scyllo-inosose
pH 7.5, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
inactivation of iolX impairs growth with scyllo-inositol as the carbon source
metabolism
the enzyme is involved in the inositol metabolic pathway in Bacillus subtilis, organization of the relevant genes, overview
physiological function
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a C-terminally His6-tagged fusion protein
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme is prominently expressed when Bacillus subtilis is grown on medium containing scyllo-inositol
gene iolQ encodes a repressor of the iolX transcription, gene iolQ is organized in an operon immediately upstream of iolX, the repressor binds to the promoter region of iolX
induced during growth on scyllo-inositol
myo- and scyllo-inositol are intracellular inducers of gene iolX, but they fail to antagonize DNA binding of IolQ in in vitro experiments
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Morinaga, T.; Ashida, H.; Yoshida, K.
Identification of two scyllo-inositol dehydrogenases in Bacillus subtilis
Microbiology
156
1538-1546
2010
Bacillus subtilis (P40332), Bacillus subtilis 168 (P40332)
Manually annotated by BRENDA team
Kang, D.; Michon, C.; Morinaga, T.; Tanaka, K.; Takenaka, S.; Ishikawa, S.; Yoshida, K.
Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD+-dependent scyllo-inositol dehydrogenase
BMC Microbiol.
17
154
2017
Bacillus subtilis (P40332), Bacillus subtilis, Bacillus subtilis 168 (P40332)
Manually annotated by BRENDA team