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Information on EC 1.1.1.352 - 5'-hydroxyaverantin dehydrogenase and Organism(s) Aspergillus parasiticus and UniProt Accession P87017

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IUBMB Comments
Isolated from the aflatoxin-producing mold Aspergillus parasiticus . Involved in aflatoxin biosynthesis. 5'-Oxoaverantin will spontaneously form averufin by intramolecular ketalisation. cf. EC 4.2.1.142, 5'-oxoaverantin cyclase.
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This record set is specific for:
Aspergillus parasiticus
UNIPROT: P87017
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The taxonomic range for the selected organisms is: Aspergillus parasiticus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
havn dehydrogenase, 5'-hydroxyaverantin dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
HAVN dehydrogenase
SYSTEMATIC NAME
IUBMB Comments
(1'S,5'S)-hydroxyaverantin:NAD+ oxidoreductase
Isolated from the aflatoxin-producing mold Aspergillus parasiticus [2]. Involved in aflatoxin biosynthesis. 5'-Oxoaverantin will spontaneously form averufin by intramolecular ketalisation. cf. EC 4.2.1.142, 5'-oxoaverantin cyclase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-hydroxyaverantin + NAD(P)+
5'-oxoaverantin + NAD(P)H + H+
show the reaction diagram
-
-
-
?
(1'S,5'S)-hydroxyaverantin + NAD+
5'-oxoaverantin + NADH + H+
show the reaction diagram
-
-
-
-
?
5'-hydroxyaverantin + NAD+
5'-oxoaverantin + NADH + H+
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5'-hydroxyaverantin + NAD+
5'-oxoaverantin + NADH + H+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4,6-dihydroxy-5-methoxy-6a-methylcyclohexa[de]indano-[7,6-e]cyclopenta[c]2H-pyran-1,9-dione
-
TAEMC161, specific inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.083
5'-hydroxyaverantin
-
in 60 mM potassium phosphate buffer (pH 7.5), at 30°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
4,6-dihydroxy-5-methoxy-6a-methylcyclohexa[de]indano-[7,6-e]cyclopenta[c]2H-pyran-1,9-dione
Aspergillus parasiticus
-
in 50 mM Tris-HCl buffer (pH 8.5), at 33°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000032
-
crude enzyme, in 60 mM potassium phosphate buffer (pH 7.5), at 30°C
0.0222
-
after 694fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
2 * 28000, SDS-PAGE
29600
-
2 * 29600, calculated from amino acid sequence
60000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, butyl-Toyopearl 650 M column chromatography
-
ammonium sulfate precipitation, DE 52 column chromatography, butyl Toyopearl column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chang, P.K.; Yu, J.; Ehrlich, K.C.; Boue, S.M.; Montalbano, B.G.; Bhatnagar, D.; Cleveland, T.E.
adhA in Aspergillus parasiticus is involved in conversion of 5-hydroxyaverantin to averufin
Appl. Environ. Microbiol.
66
4715-4719
2000
Aspergillus parasiticus (P87017), Aspergillus parasiticus SRRC 2043 (P87017), Aspergillus parasiticus SU-1 (P87017)
Manually annotated by BRENDA team
Sakuno, E.; Yabe, K.; Nakajima, H.
Involvement of two cytosolic enzymes and a novel intermediate, 5-oxoaverantin, in the pathway from 5-hydroxyaverantin to averufin in aflatoxin biosynthesis
Appl. Environ. Microbiol.
69
6418-6426
2003
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Manually annotated by BRENDA team
Sakuno, E.; Yabe, K.; Hamasaki, T.; Nakajima, H.
A new inhibitor of 5-hydroxyaverantin dehydrogenase, an enzyme involved in aflatoxin biosynthesis, from Trichoderma hamatum
J. Nat. Prod.
63
1677-1678
2000
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Manually annotated by BRENDA team