Information on EC 1.1.1.310 - (S)-sulfolactate dehydrogenase

for references in articles please use BRENDA:EC1.1.1.310
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Chromohalobacter salexigens

top print hide
EC NUMBER
COMMENTARY hide
1.1.1.310
-
top print hide
RECOMMENDED NAME
GeneOntology No.
(S)-sulfolactate dehydrogenase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2S)-3-sulfolactate + NAD+ = 3-sulfopyruvate + NADH + H+
show the reaction diagram
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfolactate degradation I
-
-
Cysteine and methionine metabolism
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
(2S)-sulfolactate:NAD+ oxidoreductase
This enzyme, isolated from the bacterium Chromohalobacter salexigens DSM 3043, acts only on the (S)-enantiomer of 3-sulfolactate. Combined with EC 1.1.1.338, (2R)-3-sulfolactate dehydrogenase (NADP+), it provides a racemase system that converts (2S)-3-sulfolactate to (2R)-3-sulfolactate, which is degraded further by EC 4.4.1.24, (2R)-sulfolactate sulfo-lyase. The enzyme is specific for NAD+.
top
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-3-sulfolactate + NAD+
3-sulfopyruvate + NADH + H+
show the reaction diagram
3-sulfopyruvate + NADH + H+
(2S)-3-sulfolactate + NAD+
show the reaction diagram
additional information
?
-
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
(2S)-3-sulfolactate
-
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
0.14
3-sulfopyruvate
-
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
2
NAD+
-
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
0.05
NADH
-
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
-
crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
7.4
-
crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
675
-
enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
top print hide
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
-
2 * 34000, SDS-PAGE
61000
-
native enzyme, gel filtration
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
top print hide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
top print hide
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration
-
top
Show AA Sequence (105 entries)
Please use the Sequence Search for a specific query.
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.310)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)