Any feedback?
Information on EC 1.1.1.31 - 3-hydroxyisobutyrate dehydrogenase
for references in articles please use BRENDA:EC1.1.1.31Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.1.1.31 3-hydroxyisobutyrate dehydrogenaseSpecify your search results
Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
3-hydroxyisobutyrate dehydrogenase, hibadh, hibdh, 3hibdh-iv, beta-hydroxyisobutyrate dehydrogenase, 3-hibadh, athdh1, 3-hydroxyisobutyric acid dehydrogenase, 3hibdhi, at4g20930, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3-HIBADH
3-hydroxyisobutyrate dehydrogenase
3-hydroxyisobutyrate dehydrogenase-I
3-hydroxyisobutyric acid dehydrogenase
3HIBDH-IV
3HIBDHI
beta-hydroxyisobutyrate dehydrogenase
-
-
-
-
HIBADH
3-hydroxyisobutyrate dehydrogenase
-
cf. EC1.1.1.59, wide substrate specificity (hydroxyacid derivatives)
3-hydroxyisobutyrate dehydrogenase
-
cf. EC1.1.1.59, wide substrate specificity (hydroxyacid derivatives)
-
HIBADH
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
3-hydroxy-2-methylpropanoate:NAD+ oxidoreductase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9028-39-1
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-3-hydroxyisobutyrate + NAD+
methylmalonate semialdehyde + NADH + H+
-
-
-
-
r
(S)-3-hydroxyisobutyrate + NAD+
methylmalonate semialdehyde + NADH + H+
100% activity
-
-
?
(S)-3-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
?
(S)-beta-hydroxyisobutyrate + NADP+
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
-
r
(S)-methyl-3-hydroxy-2-methylpropionate + NAD+
?
1.2% activity compared to (S)-3-hydroxyisobutyrate
-
-
?
3-hydroxyisobutyrate + NAD+
methylmalonate semialdehyde + NADH + H+
-
-
-
-
r
3-hydroxypropanoic acid + NAD+
3-oxopropanoate + NADH + H+
substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate
-
-
?
3-hydroxypropionate + NAD+
malonate semialdehyde + NADH + H+
13% activity compared to (S)-3-hydroxyisobutyrate
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropionate + NADH
-
-
-
-
?
D-3-hydroxyisobutyrate + NAD+
(S)-methylmalonate semialdehyde + NADH + H+
-
-
-
-
r
D-3-hydroxyisobutyrate + NAD+
methylmalonate semialdehyde + NADH + H+
-
-
-
-
r
L-3-hydroxyisobutyrate + NAD+
(S)-methylmalonate semialdehyde + NADH + H+
-
-
-
-
r
L-3-hydroxyisobutyrate + NAD+
methylmalonate semialdehyde + NADH + H+
-
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
(R)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
2% activity compared to (S)-3-hydroxyisobutyrate
-
-
?
(R)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate
-
-
?
(R)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
the enzyme is very specific to (S)-3-hydroxyisobutyrate
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
the enzyme is specific to (S)-3-hydroxyisobutyrate
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
the enzyme is specific to (S)-3-hydroxyisobutyrate
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
the enzyme is very specific to (S)-3-hydroxyisobutyrate
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
the enzyme is very specific to (S)-3-hydroxyisobutyrate
-
-
?
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
?
3-hydroxy-2-methylpropanoate + NADP+
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
-
r
3-hydroxypropanoate + NAD(P)+
?
-
is more active with NADP+ than NAD+
-
-
?
3-hydroxypropionic acid + NAD+
malonate semialdehyde + NADH + H+
-
-
-
-
r
3-hydroxypropionic acid + NAD+
malonate semialdehyde + NADH + H+
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-
-
r
D,L-3-hydroxyisobutyrate + NAD+
D-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
D-2-methyl-3-oxopropionate + NADH
-
strain IFO 0750
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
strain IFO 1542
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
Pseudomonas dacunhae
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
3-oxoisobutyrate + NADH
high stereospecificity
-
-
r
L-3-hydroxyisobutyrate + NAD+
3-oxoisobutyrate + NADH
high stereospecificity
-
-
r
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
Corynebacterium hydrocarbons
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
r
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-serine + NAD+
3-oxo-L-alanine + NADH + H+
-
-
-
-
r
L-serine + NAD+
3-oxo-L-alanine + NADH + H+
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-
-
r
L-serine + NAD+
?
substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate
-
-
?
L-serine + NAD+
?
substrate with much less efficiency compared to (S)-3-hydroxyisobutyrate
-
-
?
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-
-
r
methyl DL-serine + NAD+
methyl 3-oxo-DL-alanine + NADH + H+
-
-
-
-
r
methyl DL-serine + NAD+
methyl 3-oxo-DL-alanine + NADH + H+
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
-
-
-
r
additional information
?
-
-
no activity with (R)-methyl-3-hydroxy-2-methylpropionate, glycerate, serine, beta-hydroxypyruvate, threonine, 2-hydroxybutyrate, malate, glycolate, beta-hydroxybutyrate, lactate, and 6-phosphogluconate. NADP+ is not a suitable cofactor. Also, FAD, oxidized cytochrome c, as well as the artificial electron acceptors phenazine methosulfate and dichlorophenol indophenol cannot be reduced by the enzyme
-
-
-
additional information
?
-
no activity with (R)-methyl-3-hydroxy-2-methylpropionate, glycerate, serine, beta-hydroxypyruvate, threonine, 2-hydroxybutyrate, malate, glycolate, beta-hydroxybutyrate, lactate, and 6-phosphogluconate. NADP+ is not a suitable cofactor. Also, FAD, oxidized cytochrome c, as well as the artificial electron acceptors phenazine methosulfate and dichlorophenol indophenol cannot be reduced by the enzyme
-
-
-
additional information
?
-
-
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
-
-
?
additional information
?
-
-
wide substrate specificity of the 3-HIBADH
-
-
?
additional information
?
-
-
no activity with NADP+, DL-glycerate, (R)-3-hydroxyisobutyrate, L-threonine, D-threonine, and D-serine
-
-
?
additional information
?
-
-
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
-
-
?
additional information
?
-
-
wide substrate specificity of the 3-HIBADH
-
-
?
additional information
?
-
no activity with D-serine, (S)-3-hydroxyisobutyrate methyl ester, (R)-3-hydroxyisobutyrate methyl ester, L-threonine and D-threonine
-
-
-
additional information
?
-
-
no activity with D-serine, (S)-3-hydroxyisobutyrate methyl ester, (R)-3-hydroxyisobutyrate methyl ester, L-threonine and D-threonine
-
-
-
additional information
?
-
no activity with D-serine, (S)-3-hydroxyisobutyrate methyl ester, (R)-3-hydroxyisobutyrate methyl ester, L-threonine and D-threonine
-
-
-
additional information
?
-
-
no activity with (R)-3-hydroxyisobutyrate
-
-
?
additional information
?
-
Pseudomonas denitrificans (nom. rej.) ATCC 13867
-
no activity with (R)-3-hydroxyisobutyrate
-
-
?
additional information
?
-
-
the enzyme catalyzes the rate-limiting step in the irreversible degradative pathway for the carbon skeleton of valine and the other branched-chain amino acids
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D,L-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropionate + NADH
-
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
the enzyme is specific to (S)-3-hydroxyisobutyrate and prefers NAD+ as cofactor
-
-
?
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
-
?
3-hydroxy-2-methylpropanoate + NADP+
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
-
r
D,L-3-hydroxyisobutyrate + NAD+
D-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
D-2-methyl-3-oxopropionate + NADH
-
strain IFO 0750
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
strain IFO 1542
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
D,L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
Pseudomonas dacunhae
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
Corynebacterium hydrocarbons
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
r
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
L-3-hydroxyisobutyrate + NAD+
L-2-methyl-3-oxopropionate + NADH
-
-
-
-
?
additional information
?
-
-
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
-
-
?
additional information
?
-
-
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
-
-
?
additional information
?
-
-
the enzyme catalyzes the rate-limiting step in the irreversible degradative pathway for the carbon skeleton of valine and the other branched-chain amino acids
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
the enzyme prefers NAD+ over NADP+ as a cofactor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
additional information
additional information
-
has no metal ion requirement, MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 have no effect
additional information
-
not influenced by Na+, K+, NH3+, and Li+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(R)-3-hydroxy-2-methylpropanoate
-
competitive inhibitor of the S-isomer
ZnCl2
-
0.2 mM, 60% inhibition; inhibits approximately 60% of 3-HIBADH activity at 0.2 mM but shows no effect at 0.005 mM
additional information
-
is not inhibited by 1-propanol, lactate potassium, malate potassium, malonate potassium, acetaldehyde, ethanol, and glycerin
-
NADH
competitive inhibition in the presence of NADH when NAD+ is varied, noncompetitive inhibition with NADH when (S)-3-hydroxyisobutyrate is varied
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
ethylene glycol tetraacetic acid or EDTA (all at 0.005 and 0.2 mM) do not affect 3-HIBADH activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
3-hydroxyisobutyrate dehydrogenase deficiency
3-Hydroxyisobutyrate dehydrogenase (HIBADH) deficiency-A novel disorder of valine metabolism.
Gastroesophageal Reflux
Duodenal lipid-induced symptom generation in gastroesophageal reflux disease: role of apolipoprotein A-IV and cholecystokinin.
Paralysis
[Mutants of bacterium Azospirillum brasilense Sp245 with Omegon insertion in mmsB or fabG genes of lipid metabolism are defective in motility and flagellation].
Tuberculosis
Role of a cysteine residue in substrate entry and catalysis in MtHIBADH: Analysis by chemical modifications and site-directed mutagenesis.
Tuberculosis
Structure, interactions and action of Mycobacterium tuberculosis 3-hydroxyisobutyric acid dehydrogenase.