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Information on EC 1.1.1.29 - glycerate dehydrogenase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9C9W5

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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9C9W5 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
d-glycerate dehydrogenase, glycerate dehydrogenase, nadh-dependent hydroxypyruvate reductase, gr/hpr, hydroxypyruvate reductase 1, glyoxalate reductase/hydroxypyruvate reductase, hydroxypyruvate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxypyruvate reductase
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D-glycerate dehydrogenase
-
-
-
-
dehydrogenase, glycerate
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-
-
-
GDH
-
-
-
-
glyoxylate reductase
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-
-
-
HPR
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-
-
-
hydroxypyruvate dehydrogenase
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-
-
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hydroxypyruvate reductase
-
-
-
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hydroxypyruvate reductase 1
-
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NADH-dependent hydroxypyruvate reductase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glycerate:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-37-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glyoxylate + NADH + H+
glycolate + NAD+
show the reaction diagram
reaction of EC 1.1.1.26
-
-
?
hydroxypyruvate + NADH + H+
D-glycerate + NAD+
show the reaction diagram
hydroxypyruvate + NADPH + H+
D-glycerate + NADP+
show the reaction diagram
NADPH is a poor cofactor for wild-type
-
-
?
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
ir
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hydroxypyruvate + NADH + H+
D-glycerate + NAD+
show the reaction diagram
-
-
-
?
hydroxypyruvate + NADH
D-glycerate + NAD+
show the reaction diagram
-
-
-
-
ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
NADPH is a poor cofactor for wild-type
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GSH
about 72% residual activity at 2 mM
S-nitrosoglutathione
about 80% residual activity at 2 mM
SIN-1
about 35% residual activity at 1 mM
additional information
not inhibited by H2O2
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.167 - 1.579
Hydroxypyruvate
0.018 - 0.028
NADH
0.102 - 0.147
NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
139 - 284
Hydroxypyruvate
144 - 248
NADH
148 - 230
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0051
recombinant enzyme, at pH and °C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
HPR1 phosphomimetic variant T335D exhibits reduced NADH-dependent hydroxypyruvate reductase activity but improved NADPH-dependent activity. Complementation of the Arabidopsis HPR1 mutant by either wild-type HPR1 or HPR1 nonphosphorylatable mutant T335A fully complements the photorespiratory growth phenotype of the HPR1 mutant in ambient air, whereas HPR1 T335D-containing HPR1 mutant plants remain smaller and have lower photosynthetic CO2 assimilation rates. These phenotypes were associated with subtle perturbations in the photorespiratory cycle of HPR1 T335D-complemented HPR1 mutant rosettes compared to all other HPR1-containing lines
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HPR1_ARATH
386
0
42248
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T335A
nonphosphorylatable variant
T335D
phosphomimetic variant, exhibits reduced NADH-dependent hydroxypyruvate reductase activity while showing improved NADPH-dependent activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
LYTRA column chromatography, and Superdex 200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BIVU0811
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cousins, A.B.; Walker, B.J.; Pracharoenwattana, I.; Smith, S.M.; Badger, M.R.
Peroxisomal hydroxypyruvate reductase is not essential for photorespiration in Arabidopsis but its absence causes an increase in the stoichiometry of photorespiratory CO2 release
Photosynth. Res.
108
91-100
2011
Arabidopsis thaliana (Q9C9W5)
Manually annotated by BRENDA team
Kern, R.; Bauwe, H.; Hagemann, M.
Evolution of enzymes involved in the photorespiratory 2-phosphoglycolate cycle from cyanobacteria via algae toward plants
Photosynth. Res.
109
103-114
2011
Arabidopsis thaliana
Manually annotated by BRENDA team
Corpas, F.J.; Leterrier, M.; Begara-Morales, J.C.; Valderrama, R.; Chaki, M.; Lopez-Jaramillo, J.; Luque, F.; Palma, J.M.; Padilla, M.N.; Sanchez-Calvo, B.; Mata-Perez, C.; Barroso, J.B.
Inhibition of peroxisomal hydroxypyruvate reductase (HPR1) by tyrosine nitration
Biochim. Biophys. Acta
1830
4981-4989
2013
Arabidopsis thaliana (Q9C9W5)
Manually annotated by BRENDA team
Hoover, G.J.; Jorgensen, R.; Rochon, A.; Bajwa, V.S.; Merrill, A.R.; Shelp, B.J.
Identification of catalytically important amino acid residues for enzymatic reduction of glyoxylate in plants
Biochim. Biophys. Acta
1834
2663-2671
2013
Arabidopsis thaliana (Q9C9W5)
Manually annotated by BRENDA team
Li, J.; Weraduwage, S.M.; Preiser, A.L.; Tietz, S.; Weise, S.E.; Strand, D.D.; Froehlich, J.E.; Kramer, D.M.; Hu, J.; Sharkey, T.D.
A cytosolic bypass and G6P shunt in plants lacking peroxisomal hydroxypyruvate reductase
Plant Physiol.
180
783-792
2019
Arabidopsis thaliana (Q9C9W5)
Manually annotated by BRENDA team
Liu, Y.; Guerard, F.; Hodges, M.; Jossier, M.
Phosphomimetic T335D mutation of hydroxypyruvate reductase 1 modifies cofactor specificity and impacts Arabidopsis growth in air
Plant Physiol.
183
194-205
2020
Arabidopsis thaliana (Q9C9W5), Arabidopsis thaliana
Manually annotated by BRENDA team