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IUBMB Comments Also acts on ethyl (R )-3-oxobutanoate and some other (R )-3-hydroxy acid esters. The (R )- symbol is allotted on the assumption that no substituents change the order of priority from O-3 > C-2 > C-4. A subunit of yeast fatty acid synthase EC 2.3.1.86 , fatty-acyl-CoA synthase system. cf . EC 1.1.1.280 , (S )-3-hydroxyacid ester dehydrogenase.
The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae
Synonyms EC 1.2.1.55, reductase, 3-oxo ester (R)-, more
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EC 1.2.1.55
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formerly
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reductase, 3-oxo ester (R)-
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ethyl (R)-3-hydroxyhexanoate + NADP+ = ethyl 3-oxohexanoate + NADPH + H+
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ethyl-(R)-3-hydroxyhexanoate:NADP+ 3-oxidoreductase
Also acts on ethyl (R)-3-oxobutanoate and some other (R)-3-hydroxy acid esters. The (R)- symbol is allotted on the assumption that no substituents change the order of priority from O-3 > C-2 > C-4. A subunit of yeast fatty acid synthase EC 2.3.1.86, fatty-acyl-CoA synthase system. cf. EC 1.1.1.280, (S)-3-hydroxyacid ester dehydrogenase.
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ethyl 3-oxobutanoate + NADPH
ethyl (R)-3-hydroxybutanoate + NADP+
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Substrates: - Products: -
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ethyl 3-oxohexanoate + NADPH
ethyl (R)-3-hydroxyhexanoate + NADP+
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Substrates: - Products: -
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additional information
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Substrates: specific for 3-oxo-esters, no reaction with 3-oxo-acids Products: -
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8-hydroxyquinoline 5-sulfonic acid
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17
ethyl 3-oxobutanoate
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2
ethyl 3-oxohexanoate
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7.5
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rapid decrease of activity above
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brenda
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Highest Expressing Human Cell Lines
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200000
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x * 210000 + x * 200000, SDS-PAGE
210000
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x * 210000 + x * 200000, SDS-PAGE
780000 - 800000
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gel filtration
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oligomer
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x * 210000 + x * 200000, SDS-PAGE
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using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography
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Heidlas, J.; Engel, K.H.; Tressl, R.
Purification and characterization of two oxidoreductases involved in the enantioselective reduction of 3-oxo, 4-oxo and 5-oxo esters in bakers yeast
Eur. J. Biochem.
172
633-639
1988
Saccharomyces cerevisiae
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