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Information on EC 1.1.1.270 - 3beta-hydroxysteroid 3-dehydrogenase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q12452

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IUBMB Comments
The enzyme acts on multiple 3beta-hydroxysteroids. Participates in the biosynthesis of zemosterol and cholesterol, where it catalyses the reaction in the opposite direction to that shown. The mammalian enzyme is bifunctional and also catalyses EC 1.1.1.62, 17beta-estradiol 17-dehydrogenase .
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Saccharomyces cerevisiae
UNIPROT: Q12452
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
3beta-hydroxysteroid dehydrogenase, hsd3b1, akr1c2, hsd17b7, erg27, 3beta-hsd1, 3-ketosteroid reductase, 3beta-hsd2, 3-ketoreductase, erg27p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-keto reducing enzyme
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3-keto reductase
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3-keto-reductase
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3-ketoreductase
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3-ketosteroid reductase
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3-KSR
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3-oxo steroid reductase
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3-oxosteroid reductase
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reductase, 3-oxo steroid
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steroid-3-ketoreductase
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sterone-reducing enzyme
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
3beta-hydroxysteroid:NADP+ 3-oxidoreductase
The enzyme acts on multiple 3beta-hydroxysteroids. Participates in the biosynthesis of zemosterol and cholesterol, where it catalyses the reaction in the opposite direction to that shown. The mammalian enzyme is bifunctional and also catalyses EC 1.1.1.62, 17beta-estradiol 17-dehydrogenase [4].
CAS REGISTRY NUMBER
COMMENTARY hide
42616-29-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4alpha-methylzymosterol + NADP+
3-dehydro-4-methylzymosterol + NADPH + H+
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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loss of the 3-keto reductase also results in a concomitant loss of activity of the upstream oxidosqualene cyclase. Oxidosqualene cyclase is not required for the 3-ketoreductase activity. In Saccharomyces cerevisiae, the protective relationship between the 3-keto reductase Erg27 and oxidosqualene cyclase Erg7p is not reciprocal
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K206A
strain can be used to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially
R40A
strain can be used as a source of 4-methylzymosterone
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
used of mutated 3-ketosteroid reductase (Erg27) to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially. The strategies are either incubation of cell homogenates of an Erg27-deletant strain with radioactive lanosterol, or incubation of growing cells of a strain expressing point-mutated 3-ketosteroid reductase with radioactive acetate. Chemical reduction of both radioactive and non-radioactive 3-keto sterones gives the physiological 3-beta-OH sterols, as well as the non-physiological 3-alpha-OH isomers
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Taramino, S.; Valachovic, M.; Oliaro-Bosso, S.; Viola, F.; Teske, B.; Bard, M.; Balliano, G.
Interactions of oxidosqualene cyclase (Erg7p) with 3-keto reductase (Erg27p) and other enzymes of sterol biosynthesis in yeast
Biochim. Biophys. Acta
1801
156-162
2010
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Mo, C.; Valachovic, M.; Randall, S.K.; Nickels, J.T.; Bard. M.
Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis
Proc. Natl. Acad. Sci. USA
99
39-44
2002
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Ferrante, T.; Barge, A.; Taramino, S.; Oliaro-Bosso, S.; Balliano, G.
4-Methylzymosterone and other intermediates of sterol biosynthesis from yeast mutants engineered in the ERG27 gene encoding 3-ketosteroid reductase
Lipids
51
1103-1113
2016
Saccharomyces cerevisiae (Q12452), Saccharomyces cerevisiae
Manually annotated by BRENDA team