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Information on EC 1.1.1.27 - L-lactate dehydrogenase and Organism(s) Homo sapiens

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EC Tree
IUBMB Comments
Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
A4-LDH, AdhE, anaerobic lactate dehydrogenase, BbLDH, dehydrogenase, lactate, eLDHA, eLDHB, Epsilon crystallin, epsilon-crystallin, H4-L-lactate dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenase, lactate
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Epsilon crystallin
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epsilon-crystallin
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Immunogenic protein p36
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L(+)-nLDH
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L-(+)-lactate dehydrogenase
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L-lactate dehydrogenase B
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L-lactic acid dehydrogenase
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L-lactic dehydrogenase
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L-LDH
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lactate dehydrogenase
lactate dehydrogenase 5
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lactate dehydrogenase A
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lactate dehydrogenase B
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lactate dehydrogenase NAD-dependent
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lactic acid dehydrogenase
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lactic dehydrogenase
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LDH-1
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LDH-2
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LDH-3
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LDH-4
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LDH-5
LDH-A
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NAD-lactate dehydrogenase
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proteins, specific or class, anoxic stress response, p34
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-lactate + NAD+ = pyruvate + NADH + H+
show the reaction diagram
the enzyme-NADH-pyruvate ternery complex undergoes a rate-limiting conformational change, in which the substrate loop closes to form a desolvated ternary complex in order to bring the catalytic residue Arg109 into the active site, the catalytic residues Arg109, Asp168, and His195 are highly conserved, catalyic mechanism, detailed overview
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
(S)-lactate:NAD+ oxidoreductase
Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-60-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
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r
pyruvate + NADH + H+
(S)-lactate + NAD+
show the reaction diagram
additional information
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LDH is essential for continuous glycolysis necessary for accelerated tumor growth and increased LDH activity occurs already in grade 1 EC carcinomas
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH
show the reaction diagram
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r
L-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
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r
additional information
?
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LDH is essential for continuous glycolysis necessary for accelerated tumor growth and increased LDH activity occurs already in grade 1 EC carcinomas
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(abieta-8,11,13-trien-18-ylamino)(oxo)acetic acid
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(benzylamino)(oxo)acetic acid
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(heptylamino)(oxo)acetic acid
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(hexylamino)(oxo)acetic acid
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(nonylamino)(oxo)acetic acid
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([2-cyano-4-[2-([5-hydroxy-2-[(4-methoxybenzyl)carbamoyl]-4-oxo-4H-chromen-8-yl]oxy)ethyl]phenyl]amino)(oxo)acetic acid
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([4-[2-([5-hydroxy-2-[(4-methoxybenzyl)carbamoyl]-4-oxo-4H-chromen-8-yl]oxy)ethyl]-2-methoxyphenyl]amino)(oxo)acetic acid
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([4-[2-([5-hydroxy-2-[(4-methoxybenzyl)carbamoyl]-4-oxo-4H-chromen-8-yl]oxy)ethyl]phenyl]amino)(oxo)acetic acid
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1-hydroxy-6-phenyl-4-(trifluoromethyl)-1H-indol-2-carboxylic acid
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a N-hydroxyindole, NH1-1, and a competitive inhibitor with respect to both NADH and pyruvate
1-[7-[3,4-dihydroxy-2-imino-7-methyl-5-(propan-2-yl)-2H-naphtho[1,8-bc]furan-8-yl]-2,3,8-trihydroxy-6-methyl-4-(propan-2-yl)naphthalen-1-yl]ethanone
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2,3-dihydroxy-4,6,7-trimethylnaphthalene-1-carboxylic acid
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2,3-dihydroxy-4,6-dimethylnaphthalene-1-carboxylic acid
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2,3-dihydroxy-6,7-dimethyl-4-(propan-2-yl)naphthalene-1-carboxylic acid
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2,3-dihydroxy-6,7-dimethyl-4-propylnaphthalene-1-carboxylic acid
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2,3-dihydroxy-6-methyl-4-(propan-2-yl)-7-[4-(trifluoromethyl)benzyl]naphthalene-1-carboxylic acid
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2,3-dihydroxy-6-methyl-4-(propan-2-yl)naphthalene-1-carboxylic acid
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2,3-dihydroxy-6-methyl-4-propylnaphthalene-1-carboxylic acid
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2,3-dihydroxy-6-methyl-7-(2-methylbenzyl)-4-(propan-2-yl)naphthalene-1-carboxylic acid
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2,3-dihydroxy-6-methyl-7-(3-methylbenzyl)-4-(propan-2-yl)naphthalene-1-carboxylic acid
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2,3-dihydroxy-6-methyl-7-(4-methylbenzyl)-4-(propan-2-yl)naphthalene-1-carboxylic acid
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3,5-dihydroxynaphthalene-2-carboxylic acid
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3,7-dihydroxynaphthalene-2-carboxylic acid
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3-((3-carbamoyl-7-(3,5-dimethylisoxazol-4-yl)-6-methoxyquinolin-4-yl) amino) benzoic acid
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a quinoline-3-sulfonamide, competitive inhibitor with respect to both NADH and pyruvate
3-(3-nitro-4-pyridyl)pyruvate
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3-([3-carbamoyldimethoxypyrimidin-7-(2,4-dimethoxypyrimidin-5-yl)quinolin-4-yl]amino)benzoic acid
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enzyme binding structure, overview
3-hydroxy-2-oxo-1-oxaspiro[4,5]-dec-3-ene
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3-[7-(2,4-dimethoxypyrimidin-5-yl)-3-sulfamoylquinolin-4-yl]aminobenzoic acid
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enzyme binding structure, overview
4,7-dibromo-3-hydroxynaphthalene-2-carboxylic acid
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4-(ethylcarbamoyl)benzoic acid
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6,6'-disulfanediyldipyridine-3-carboxylic acid
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6-benzyl-3,4-dihydroxy-7-methyl-1-propylnaphthalene-2-carboxylic acid
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FX11, a competitive inhibitor with respect to both NADH and pyruvate
7-(4-chlorobenzyl)-2,3-dihydroxy-6-methyl-4-(propan-2-yl)naphthalene-1-carboxylic acid
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7-benzyl-2,3-dihydroxy-4,6-dimethylnaphthalene-1-carboxylic acid
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7-benzyl-2,3-dihydroxy-6-methyl-4-(propan-2-yl)naphthalene-1-carboxylic acid
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7-benzyl-2,3-dihydroxy-6-methyl-4-propylnaphthalene-1-carboxylic acid
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8'-acetyl-1,1',6,6',7,7'-hexahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)-2,2'-binaphthalene-8-carboxylic acid
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8'-acetyl-8-cyano-1',6,6',7,7'-pentahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)-2,2'-binaphthalen-1-yl acetate
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8'-acetyl-8-cyano-1',6,6',7,7'-pentahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)-2,2'-binaphthalen-1-yl butanoate
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8'-acetyl-8-cyano-1',6,6',7,7'-pentahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)-2,2'-binaphthalen-1-yl pentanoate
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8'-acetyl-8-cyano-1',6,6',7,7'-pentahydroxy-3,3'-dimethyl-5,5'-di(propan-2-yl)-2,2'-binaphthalen-1-yl propanoate
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8-(2-[4-[(carboxycarbonyl)amino]-3-methoxyphenyl]ethoxy)-5-hydroxy-4-oxo-4H-chromene-2-carboxylic acid
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8-(phenylamino)naphthalene-1-sulfonic acid
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8-([4-[(carboxycarbonyl)amino]-3-methoxybenzyl]oxy)-5-hydroxy-4-oxo-4H-chromene-2-carboxylic acid
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8-[8-acetyl-1,6,7-trihydroxy-3-methyl-5-(propan-2-yl)naphthalen-2-yl]-3,4-dihydroxy-7-methyl-5-(propan-2-yl)-2H-naphtho[1,8-bc]furan-2-one
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amino(oxo)acetic acid
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AZ-33
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a malonic derivative, a competitive inhibitor with respect to both NADH and pyruvate
bis(acetatato-kO)(biphenyl-2,2'-diyl-k2C2,C2')copper
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bis(acetatato-kO)(biphenyl-2,2'-diyl-k2C2,C2')zinc
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Chinese gall
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ethanol extract of the Chinese gall, commonly named Wu Bei Zi, strongly inhibits the enzyme
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epigallocatechin
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the most potent compound with anti-LDH-5 activity under both normoxia and hypoxia conditions
ethyl 3-(3-cyano-4-pyridyl)pyruvate
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galloflavin
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a blocker of LDH-5-ssDNA interactions, preventing RNA synthesis
GNE-140
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a piperidine derivative LDH-5 inhibitor
gossypol
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methyl 1-hydroxy-6-phenyl-4-(trifluoromethyl)-1H-indol-2-carboxylate
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a N-hydroxyindole, NH1-2, and a competitive inhibitor with respect to both NADH and pyruvate
naphthalene-2,6-dicarboxylic acid
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naphthalene-2,6-disulfonic acid
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oxamate
oxo(pentadecylamino)acetic acid
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oxo(phenylamino)acetic acid
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oxo[(2-phenylethyl)amino]acetic acid
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oxo[(2-phenylpropyl)amino]acetic acid
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oxo[(3-phenylpropyl)amino]acetic acid
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oxo[(4-phenylbutan-2-yl)amino]acetic acid
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oxo[(4-phenylbutyl)amino]acetic acid
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oxo[(tetrahydrofuran-2-ylmethyl)amino]acetic acid
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oxo[[1-(5,6,7,8-tetrahydronaphthalen-1-yl)ethyl]amino]acetic acid
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pyruvate
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above 0.3 mM
Spatholobus suberectus extract
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the extract has a strong inhibitory effect on LDH-5 expression and activity inboth estrogen-dependent and estrogen-independent human breast cancer cells
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[(2-ethylphenyl)(phenyl)amino](oxo)acetic acid
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[(2-methoxyethyl)amino](oxo)acetic acid
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[(3,3-diphenylpropyl)amino](oxo)acetic acid
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[(3-methoxypropyl)amino](oxo)acetic acid
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[(3-methylbutyl)amino](oxo)acetic acid
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[(3-methylphenyl)(phenyl)amino](oxo)acetic acid
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[(4-chlorobenzyl)amino](oxo)acetic acid
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[(4-methylbenzyl)amino](oxo)acetic acid
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[(furan-2-ylmethyl)(methyl)amino](oxo)acetic acid
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[(naphthalen-1-ylmethyl)amino](oxo)acetic acid
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[benzyl(methyl)amino](oxo)acetic acid
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[bis(2-methylpiperidin-1-yl)amino](oxo)acetic acid
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[bis(4-benzylpiperazin-1-yl)amino](oxo)acetic acid
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[bis(4-benzylpiperidin-1-yl)amino](oxo)acetic acid
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[bis(4-phenylpiperazin-1-yl)amino](oxo)acetic acid
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[[2-(4-bromophenyl)ethyl]amino](oxo)acetic acid
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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direct phosphorylation of LDHA at Y10 and Y83 strongly enhances LDH-5 tetramer formation and cofactor binding, resulting in significantly increased LDH enzymatic activity
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