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Information on EC 1.1.1.26 - glyoxylate reductase and Organism(s) Thermococcus litoralis and UniProt Accession Q9C4M5

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EC Tree
     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.26 glyoxylate reductase
IUBMB Comments
Reduces glyoxylate to glycolate or hydroxypyruvate to D-glycerate.
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This record set is specific for:
Thermococcus litoralis
UNIPROT: Q9C4M5
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Word Map
The taxonomic range for the selected organisms is: Thermococcus litoralis
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
grhpr, glyoxylate reductase, glyr1, glyoxylate reductase/hydroxypyruvate reductase, glyr2, glyoxylate/hydroxypyruvate reductase, nadh-dependent glyoxylate reductase, nadh-glyoxylate reductase, succinic semialdehyde/glyoxylate reductase, glyoxylic acid reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NADH-dependent glyoxylate reductase
-
glycolate oxidase
-
-
-
-
glyoxylic acid reductase
-
-
-
-
NADH-dependent glyoxylate reductase
-
-
-
-
NADH-glyoxylate reductase
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-
-
-
reductase, glyoxylate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycolate:NAD+ oxidoreductase
Reduces glyoxylate to glycolate or hydroxypyruvate to D-glycerate.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-32-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycolate + NAD+
glyoxylate + NADH + H+
show the reaction diagram
-
-
-
?
glyoxylate + NADH + H+
glycolate + NAD+
show the reaction diagram
NADPH exhibits 7.5% of the activity compared to NADH
-
-
?
hydroxypyruvate + NADH + H+
D-glycerate + NAD+
show the reaction diagram
the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycolate + NAD+
glyoxylate + NADH + H+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
NADPH exhibits rather low activity as an electron donor compared with NADH
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
1 mM, 95% inhibition
CuCl2
1 mM, 66% inhibition
dithiothreitol
1 mM, 50% inhibition
HgCl2
1 mM, complete inhibition
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.73
glyoxylate
pH 6.5, 50°C
1.3
Hydroxypyruvate
pH 6.5, 50°C
0.067
NADH
pH 6.5, 50°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44.5
pH 6.5, 50°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36807
2 * 36807, calculated from sequence
37000
2 * 37000, SDS-PAGE
76000
non-denaturimg gradient PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
10 min, stable up to
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohshima, T.; Nunoura-Kominato, N.; Kudome, T.; Sakuraba, H.
A novel hyperthermophilic archaeal glyoxylate reductase from Thermococcus litoralis. Characterization, gene cloning, nucleotide sequence and expression in Escherichia coli
Eur. J. Biochem.
268
4740-4747
2001
Thermococcus litoralis (Q9C4M5), Thermococcus litoralis, Thermococcus litoralis DSM 5473 (Q9C4M5), Thermococcus litoralis DSM 5473
Manually annotated by BRENDA team