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Information on EC 1.1.1.244 - methanol dehydrogenase
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EC Tree
1.1.1.244 methanol dehydrogenaseSpecify your search results
Word Map
- 1.1.1.244
- methylobacterium
- formaldehyde
- quinone
- methane
- methanotrophs
- pqq
- extorquens
-
quinoproteins
- denitrificans
-
methylotrophy
- methylamine
-
paracoccus
-
lanthanide
- methylophilus
- hyphomicrobium
- methanolicus
- methylomonas
- methylosinus
- methylococcus
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methane-oxidizing
-
methanol-grown
- trichosporium
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lanthanide-dependent
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pqq-dependent
- methylomicrobium
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pink-pigmented
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pyrrolo-quinoline
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rump
- methylocystis
- methylophaga
- methylobacter
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xoxf-type
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pqq-containing
- organophilum
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phyllosphere
-
quinone-dependent
- methylacidiphilum
-
rare-earth
- ch3oh
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dye-linked
- biotechnology
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
methanol dehydrogenase, nad-dependent mdh, nad-dependent methanol dehydrogenase, lxmdh, methanol dehydrogenase 2, group iii nad-dependent alcohol dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
activator-independent methanol dehydrogenase
ADH
BFZC1_05383
dehydrogenase, methanol
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-
-
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group III NAD-dependent alcohol dehydrogenase
lxmdh
MDH
Mdh1
MDH2
Mdh3
MEDH
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-
-
-
methanol dehydrogenase 2
NAD+ dependent methanol dehydrogenase
NAD-dependent MDH
NAD-dependent methanol dehydrogenase
additional information
additional information
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not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8
additional information
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not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8
additional information
-
not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
methanol + NAD+ = formaldehyde + NADH + H+
ping-pong mechanism in the absence of ACT protein
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methanol + NAD+ = formaldehyde + NADH + H+
ping-pong mechanism in the absence of ACT protein
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methanol + NAD+ = formaldehyde + NADH + H+
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
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-
redox reaction
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-
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reduction
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-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
methanol:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
74506-37-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
formaldehyde + NADH + H+
methanol + NAD+
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Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
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-
r
formaldehyde + NADH + H+
methanol + NAD+
key step for both toxic formaldehyde elimination
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-
r
formaldehyde + NADH + H+
methanol + NAD+
key step for both toxic formaldehyde elimination
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
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Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
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Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
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-
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r
methanol + NAD+
formaldehyde + NADH + H+
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33% of activity with ethanol
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r
methanol + NAD+
formaldehyde + NADH + H+
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44% of activity with ethanol, NADP+ cannot replace NAD+
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?
methanol + NAD+
formaldehyde + NADH + H+
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no activity with 2-propanol, 2,3-butandiol, mannitol and glycerol
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?
methanol + NAD+
formaldehyde + NADH + H+
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involved in initial methanol oxidation
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r
methanol + NAD+
formaldehyde + NADH + H+
-
involved in initial methanol oxidation
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
involved in initial methanol oxidation
-
r
methanol + NAD+
formaldehyde + NADH + H+
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involved in initial methanol oxidation
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r
methanol + NAD+
formaldehyde + NADH + H+
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very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
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-
r
methanol + NAD+
formaldehyde + NADH + H+
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very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
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r
methanol + NAD+
formaldehyde + NADH + H+
key step for ethanol production in bacterial methylotrophy
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r
methanol + NAD+
formaldehyde + NADH + H+
key step for ethanol production in bacterial methylotrophy
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-
r
methanol + NAD+
formaldehyde + NADH + H+
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very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
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very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
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-
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r
methanol + NAD+
formaldehyde + NADH + H+
in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
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r
methanol + NAD+
formaldehyde + NADH + H+
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very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
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r
methanol + NAD+
formaldehyde + NADH + H+
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very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
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r
methanol + NAD+
formaldehyde + NADH + H+
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-
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r
methanol + NAD+
formaldehyde + NADH + H+
XoxF may not be involved in methylotrophy
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-
?
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Mdh enzyme mutant D41G, no activity of the wild-type enzyme with NADP(H)
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r
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Mdh enzyme mutant D98G, no activity of the wild-type enzyme with NADP(H)
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r
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Mdh enzyme mutant D98G, no activity of the wild-type enzyme with NADP(H)
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-
r
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Mdh enzyme mutant D41G, no activity of the wild-type enzyme with NADP(H)
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r
n-butanol + NAD+
n-butanal + NADH
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87% of activity with ethanol
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?
n-butanol + NAD+
n-butanal + NADH
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87% of activity with ethanol
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r
n-propanol + NAD+
n-propanal + NADH
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71% of activity with ethanol
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?
n-propanol + NAD+
n-propanal + NADH
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71% of activity with ethanol
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r
additional information
?
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all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
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?
additional information
?
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efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
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?
additional information
?
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I3DTM5
poor activity with 2-propanol and 1,2-popanediol
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?
additional information
?
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poor activity with 2-propanol and 1,2-popanediol
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?
additional information
?
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poor activity with 2-propanol and 1,2-popanediol
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?
additional information
?
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all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
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-
?
additional information
?
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I3DTM5
poor activity with 2-propanol and 1,2-popanediol
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?
additional information
?
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poor activity with 2-propanol and 1,2-popanediol
-
-
?
additional information
?
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efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
-
-
?
additional information
?
-
-
all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
formaldehyde + NADH + H+
methanol + NAD+
key step for both toxic formaldehyde elimination
-
-
r
formaldehyde + NADH + H+
methanol + NAD+
key step for both toxic formaldehyde elimination
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
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involved in initial methanol oxidation
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
involved in initial methanol oxidation
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
involved in initial methanol oxidation
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
involved in initial methanol oxidation
-
r
methanol + NAD+
formaldehyde + NADH + H+
in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
key step for ethanol production in bacterial methylotrophy
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
key step for ethanol production in bacterial methylotrophy
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
-
-
r
methanol + NAD+
formaldehyde + NADH + H+
-
-
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r
additional information
?
-
efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
-
-
?
additional information
?
-
efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+