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Information on EC 1.1.1.22 - UDP-glucose 6-dehydrogenase and Organism(s) Rattus norvegicus and UniProt Accession O70199

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IUBMB Comments
Also acts on UDP-alpha-D-2-deoxyglucose.
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This record set is specific for:
Rattus norvegicus
UNIPROT: O70199
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The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
udp-glucose dehydrogenase, udpgdh, udpgd, udp-glucose 6-dehydrogenase, uridine diphosphoglucose dehydrogenase, udpglucose dehydrogenase, udp-gdh, udp-glc dehydrogenase, udpg dehydrogenase, udp-glcdh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UDP-glucose dehydrogenase
-
dehydrogenase, uridine diphosphoglucose
-
-
-
-
Sugarless protein
-
-
-
-
UDP-alpha-D-glucose:NAD oxidoreductase
-
-
-
-
UDP-D-glucose dehydrogenase
-
-
-
-
UDP-Glc dehydrogenase
-
-
-
-
UDP-Glc DH
-
-
-
-
UDP-GlcDH
-
-
-
-
UDPG dehydrogenase
-
-
-
-
UDPG:NAD oxidoreductase
-
-
-
-
UDPGDH
-
-
-
-
UDPGlc dehydrogenase
-
-
-
-
UDPglucose dehydrogenase
-
-
-
-
UDPglucose:NAD+ oxidoreductase
-
-
-
-
uridine diphosphate D-glucose dehydrogenase
-
-
-
-
uridine diphosphate glucose dehydrogenase
-
-
-
-
uridine diphosphoglucose dehydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:NAD+ 6-oxidoreductase
Also acts on UDP-alpha-D-2-deoxyglucose.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-26-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 2 NAD+ + H2O
UDP-alpha-D-glucuronate + 2 NADH + 2 H+
show the reaction diagram
-
-
-
?
3-OH-benzopyrene + NAD+
?
show the reaction diagram
-
-
-
-
?
UDP-glucose + 2 NAD+ + H2O
UDP-glucuronate + 2 NADH + 2 H+
show the reaction diagram
-
-
-
-
?
UDP-glucose + nicotinamide hypoxanthine dinucleotide + H2O
UDP-glucuronate + ?
show the reaction diagram
-
-
-
-
?
UDPglucose + NAD+ + H2O
UDPglucuronate + NADH
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 2 NAD+ + H2O
UDP-alpha-D-glucuronate + 2 NADH + 2 H+
show the reaction diagram
-
-
-
?
UDPglucose + NAD+ + H2O
UDPglucuronate + NADH
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-NAD+
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal ion requirement
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NH2OH
-
deactivation
piperine
-
non-competitive, reversible, non-pH-dependent, 90% inhibition at concentration 0.05 mM and 10% inhibition at concentration 0.01 mM
UDPglucuronate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
nicotinamide hypoxanthine dinucleotide
-
can replace NAD+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.015 - 1
UDPglucose
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.6
-
two pH optima, pH 8.6 and pH 9.4
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
UGDH protein level in rat osteoarthritis cartilage are much lower than the corresponding controls and negatively correlated to the degree of osteoarthritis
physiological function
UGDH is essential in the proteoglycan synthesis in articular chondrocytes, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
UGDH_RAT
493
0
54892
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
300000
-
gel filtration
70000
-
4 * 70000, gel filtration after treatment with SDS
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 70000, gel filtration after treatment with SDS
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene UGDH, real-time quantitative PCR enzyme expression analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sivaswami, A.; Kelkar, S.M.; Nadkarni, G.B.
Uridine diphosphoglucose dehydrogenase from rat liver: purification and effect of pH on regulatory properties
Biochim. Biophys. Acta
276
43-52
1972
Rattus norvegicus
Manually annotated by BRENDA team
Molz, R.J.; Danishefsky, I.
Uridine diphosphate glucose dehydrogenase of rat tissue
Biochim. Biophys. Acta
250
6-13
1971
Rattus norvegicus
Manually annotated by BRENDA team
Reen, R.K.; Jamwal, D.S.; Taneja, S.C.; Koul, J.L.; Dubey, R.K.; Wiebel, F.J.; Singh, J.
Impairment of UDP-glucose dehydrogenase and glucuronidation activities in liver and small intestine of rat and guinea pig in vitro by piperine
Biochem. Pharmacol.
46
229-238
1993
Cavia porcellus, Rattus norvegicus
Manually annotated by BRENDA team
Wen, Y.; Li, J.; Wang, L.; Tie, K.; Magdalou, J.; Chen, L.; Wang, H.
UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis
Arthritis Res. Ther.
16
484-494
2014
Homo sapiens (O60701), Rattus norvegicus (O70199), Rattus norvegicus Wistar (O70199)
Manually annotated by BRENDA team