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Information on EC 1.1.1.219 - dihydroflavonol 4-reductase and Organism(s) Callistephus chinensis and UniProt Accession P51103

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IUBMB Comments
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
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This record set is specific for:
Callistephus chinensis
UNIPROT: P51103
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Word Map
The taxonomic range for the selected organisms is: Callistephus chinensis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
dihydroflavonol 4-reductase, flavonoid 3'-hydroxylase, dihydroflavonol reductase, dihydroflavonol-4-reductase, csdfr, tadfr, mcdfr, brdfr1, bo9g058630, pgdfr, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dihydroflavonol 4-reductase
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DFR
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-
-
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DHM reductase
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-
-
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DHQ reductase
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-
-
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dihydroflavanol 4-reductase
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-
-
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dihydrokaempferol 4-reductase
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-
-
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dihydromyricetin reductase
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-
-
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dihydroquercetin reductase
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-
-
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NADPH-dihydromyricetin reductase
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-
-
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reductase, dihydromyricetin
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-
-
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reductase, dihydroquercetin
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-
-
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TRANSPARENT TESTA 3 protein
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
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oxidation
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-
-
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reduction
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-
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
83682-99-9
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98668-58-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R,3R)-(+)-dihydrokaempferol + NADPH
(2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
show the reaction diagram
-
-
-
?
(2R,3R)-dihydromyricetin + NADPH
(2R,3S,4R)-3,4-leucodelphinidin + NADP+
show the reaction diagram
-
-
-
?
(2R,3R)-dihydroquercetin + NADPH
(2R,3S,4R)-leucocyanidin + NADP+
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
line 101
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DFRA_CALCH
364
0
40726
Swiss-Prot
other Location (Reliability: 3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Martens, S.; Teeri, T.; Forkmann, G.
Heterologous expression of dihydroflavonol 4-reductases from various plants
FEBS Lett.
531
453-458
2002
Matthiola incana, no activity in Saccharomyces cerevisiae, no activity in Escherichia coli, no activity in Nicotiana tabacum, Callistephus chinensis (P51103), Dianthus caryophyllus (P51104), Gerbera hybrid cultivar (P51105), Solanum lycopersicum (P51107), Rosa hybrid cultivar (Q41158), Rosa hybrid cultivar Kardinal (Q41158)
Manually annotated by BRENDA team