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Information on EC 1.1.1.216 - farnesol dehydrogenase (NADP+) and Organism(s) Aedes aegypti and UniProt Accession D2WKD9

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EC Tree
IUBMB Comments
Also acts, more slowly, on (2Z,6E)-farnesol, geraniol, citronerol and nerol.
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This record set is specific for:
Aedes aegypti
UNIPROT: D2WKD9
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The taxonomic range for the selected organisms is: Aedes aegypti
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
sdr-1, nad(p)+-dependent farnesol dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NADP+-dependent farnesol dehydrogenase 1
-
dehydrogenase, farnesol (nicotinamide adenine dinucleotide phosphate)
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-
-
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NADP-farnesol dehydrogenase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
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oxidation
-
-
-
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reduction
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2E,6E)-farnesol:NADP+ 1-oxidoreductase
Also acts, more slowly, on (2Z,6E)-farnesol, geraniol, citronerol and nerol.
CAS REGISTRY NUMBER
COMMENTARY hide
90804-55-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesol + NADP+
(2E,6E)-farnesal + NADPH + H+
show the reaction diagram
enzyme shows highest affinity for farnesol
-
-
ir
(2Z,6Z)-farnesol + NADP+
(2Z,6Z)-farnesal + NADPH + H+
show the reaction diagram
enzyme shows highest affinity for farnesol
-
-
r
(E)-geraniol + NADP+
(E)-geranial + NADPH + H+
show the reaction diagram
-
-
-
?
(Z)-nerol + NADP+
(Z)-neral + NADPH + H+
show the reaction diagram
-
-
-
?
2-decanol + NADP+
? + NADPH + H+
show the reaction diagram
-
-
-
?
citronellol + NADP+
citronellal + NADPH + H+
show the reaction diagram
-
-
-
?
octanol + NADP+
octanal + NADPH + H+
show the reaction diagram
-
-
-
?
additional information
?
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no activity is detected for butanol, ethanol, or glycerol, or for farnesal or farnesoic acid
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
absolutely required, NAD+ or FAD cannot substitute
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.091
(2E,6E)-farnesol
pH 10.0, 30°C
0.097
(2Z,6Z)-farnesol
pH 10.0, 30°C
0.208
(E)-geraniol
pH 10.0, 30°C
0.109
(Z)-nerol
pH 10.0, 30°C
0.184
2-decanol
pH 10.0, 30°C
0.133
citronellol
pH 10.0, 30°C
0.195
Octanol
pH 10.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
substrate (2Z,6Z)-farnesol, pH 10.0, 30°C
9.7
substrate (2E,6E)-farnesol, pH 10.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NADP+-dependent farnesol dehydrogenase 1
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
and midgut, of adult female, highest levels of mRNA
Manually annotated by BRENDA team
no farnesol dehydrogenase activity in newly emerged mosquitoes but significant activity 24 h later. SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
Manually annotated by BRENDA team
and Malpighian tubules, considerable levles of mRNA
Manually annotated by BRENDA team
abdominal and thoracic ganglia, high level of mRNA
Manually annotated by BRENDA team
and fat body, considerable levles of mRNA
Manually annotated by BRENDA team
and brain, of adult female, highest levels of mRNA
Manually annotated by BRENDA team
of adult male, substantial level of mRNA
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SDR1_AEDAE
245
0
26542
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
2 * 29000, calculated
60000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 29000, calculated
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mayoral, J.G.; Nouzova, M.; Navare, A.; Noriega, F.G.
NADP+-dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis
Proc. Natl. Acad. Sci. USA
106
21091-21096
2009
Aedes aegypti (D2WKD9)
Manually annotated by BRENDA team