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Information on EC 1.1.1.194 - coniferyl-alcohol dehydrogenase

for references in articles please use BRENDA:EC1.1.1.194

Word Map on EC 1.1.1.194

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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1.1.1.194

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coniferyl-alcohol dehydrogenase

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coniferyl alcohol + NADP+ = coniferyl aldehyde + NADPH + H+

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oxidation

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redox reaction

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reduction

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capsiconiate biosynthesis

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phenylpropanoid biosynthesis

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coniferyl-alcohol:NADP+ oxidoreductase

Specific for coniferyl alcohol; does not act on cinnamyl alcohol, 4-coumaryl alcohol or sinapyl alcohol.

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Show Synonyms (5 entries)

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37250-27-4

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Manually annotated by BRENDA team

L. var. Mandarin

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Manually annotated by BRENDA team

Medicago sativa

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Manually annotated by BRENDA team

Metasequoia glyptostroboides

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Manually annotated by BRENDA team

L. deepwater rice

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Manually annotated by BRENDA team

Petunia x hybrida

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Manually annotated by BRENDA team

Pinus palustris

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Manually annotated by BRENDA team

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Manually annotated by BRENDA team

Rhodococcus sp.

gene calA

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Manually annotated by BRENDA team

Rhodococcus sp. HR199

gene calA

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Manually annotated by BRENDA team

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Manually annotated by BRENDA team

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Manually annotated by BRENDA team

Schinus terebinthifolia

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Manually annotated by BRENDA team

Streptomyces sp.

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UniProt

Manually annotated by BRENDA team

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Manually annotated by BRENDA team

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coniferyl alcohol + NADP+

coniferyl aldehyde + NADPH + H+

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coniferyl alcohol + NADP+

coniferyl aldehyde + NADPH + H+

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NADP+

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NADPH

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additional information

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Zn2+

Streptomyces sp.

dependent on

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0.28 - 0.83

coniferyl alcohol

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0.0074

NADP+

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25.8

coniferyl alcohol

Streptomyces sp.

recombinant enzyme, at pH 8.5 and 40°C

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93.3

coniferyl alcohol

Streptomyces sp.

recombinant enzyme, at pH 8.5 and 40°C

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0.27

Streptomyces sp.

cell-free extract, at pH 8.5 and 40°C

0.36

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10.7

Streptomyces sp.

after 40fold purification, at pH 8.5 and 40°C

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8.5

Streptomyces sp.

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9.2

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25

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assay at

40

Streptomyces sp.

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cell suspension culture

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Manually annotated by BRENDA team

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39000

Streptomyces sp.

4 * 39000, SDS-PAGE

43000

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gel filtration

150000

Streptomyces sp.

gel filtration

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homotetramer

Streptomyces sp.

4 * 37845, calculated from amino acid sequence; 4 * 39000, SDS-PAGE

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-20°C, 42 mM 2-mercaptoethanol, 10% ethyleneglycol, several weeks

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ammonium sulfate precipitation, Resource PHE column chromatography, and Mono Q Superdex 200 column chromatography

Streptomyces sp.

var. Mandarin

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expressed in Escherichia coli BL21(DE3) cells

Streptomyces sp.

gene calA, co-expression of the enzyme from strain HR199 with Rhodococcus sp.strain HR199 coniferyl aldehyde dehydrogenase, and Penicillium simplicissimus strain CBS vanillyl alcohol oxidase in the deficient Rhodococcus opacus strain PD630, subcloning in Escherichia coli srain XL 1-Blue

Rhodococcus sp.

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biotechnology

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nutrition

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synthesis

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Show AA Sequence (12 entries)

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Show References (8 entries)

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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)