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Information on EC 1.1.1.175 - D-xylose 1-dehydrogenase and Organism(s) Caulobacter vibrioides and UniProt Accession B8H1Z0

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Caulobacter vibrioides
UNIPROT: B8H1Z0 not found.
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The taxonomic range for the selected organisms is: Caulobacter vibrioides
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
d-xylose dehydrogenase, d-xylose 1-dehydrogenase, d-xdh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-xylose 1-dehydrogenase
-
(NAD)-linked D-xylose dehydrogenase
-
-
-
-
D-xylose dehydrogenase
NAD+-dependent xylose dehydrogenase
-
-
NAD-D-xylose dehydrogenase
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-
-
-
additional information
-
the enzyme belongs to the short-chain dehydrogenase/reductase family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
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reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
D-xylose:NAD+ 1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
62931-20-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-xylose + NAD+
D-xylonolactone + NADH + H+
show the reaction diagram
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
-
-
-
?
D-xylono-1,4-lactone + NADH + H+
D-xylose + NAD+
show the reaction diagram
-
-
-
-
r
D-xylose + NAD+
D-xylono-1,4-lactone + NADH + H+
show the reaction diagram
-
-
-
-
r
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
show the reaction diagram
D-xylose + NAD+
D-xylonolactone + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
L-arabinose is a poor substrate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-xylose + NAD+
D-xylonolactone + NADH + H+
show the reaction diagram
D-xylono-1,4-lactone + NADH + H+
D-xylose + NAD+
show the reaction diagram
-
-
-
-
r
D-xylose + NAD+
D-xylono-1,4-lactone + NADH + H+
show the reaction diagram
-
-
-
-
r
D-xylose + NAD+
D-xylono-1,5-lactone + NADH + H+
show the reaction diagram
-
the enzyme is involved in the pathway for D-xylose metabolism, overview
-
-
r
D-xylose + NAD+
D-xylonolactone + NADH + H+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
dependent on, specific for
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 0.4
D-xylose
40 - 180
L-arabinose
0.26 - 0.46
NAD+
75
D-xylono-1,4-lactone
-
at pH 7.0 and 25°C
0.76 - 0.77
D-xylose
0.043
NAD+
-
at pH 7.0 and 25°C
0.0103
NADH
-
at pH 7.0 and 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22.7 - 30.3
D-xylose
22.3 - 29.2
L-arabinose
25.8 - 35.7
NAD+
1.44
D-xylono-1,4-lactone
-
at pH 7.0 and 25°C
16.6
D-xylose
-
at pH 7.0 and 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
66.7 - 280
D-xylose
0.12 - 0.6
L-arabinose
66.7 - 115
NAD+
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16
NAD+
-
at pH 7.0 and 25°C
0.0192
NADH
-
at pH 7.0 and 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
in vivo assay, cell growth at 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
gene xylB, recombinnat expression in Pichia kudriavzevii strain VTT C-79090T
gene xylB, recombinnat expression in Saccharomyces cerevisiae strain VVT B-67002
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
expression of gene xylB in Saccharomyces cerevisiae results in production of 17 g D-xylonate/l at 0.23g/l/h from 23 g D-xylose/l. D-Xylonate accumulates intracellularly to 70 mg/g, xylitol to 18 mg/g. Cells expressing D-xylonolactone lactonase xylC from Caulobacter crescentus with xylB initially produce more extracellular D-xylonate than cells lacking xylC at both pH5.5 and pH3, and sustain higher production at pH3. Cell vitality and viability decreases during D-xylonate production at pH 3.0
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Stephens, C.; Christen, B.; Fuchs, T.; Sundaram, V.; Watanabe, K.; Jenal, U.
Genetic analysis of a novel pathway for D-xylose metabolism in Caulobacter crescentus
J. Bacteriol.
189
2181-2185
2007
Caulobacter vibrioides, Caulobacter vibrioides NA1000
Manually annotated by BRENDA team
Toivari, M.; Nygard, Y.; Kumpula, E.P.; Vehkomaeki, M.L.; Bencina, M.; Valkonen, M.; Maaheimo, H.; Andberg, M.; Koivula, A.; Ruohonen, L.; Penttilae, M.; Wiebe, M.G.
Metabolic engineering of Saccharomyces cerevisiae for bioconversion of D-xylose to D-xylonate
Metab. Eng.
14
427-436
2012
Caulobacter vibrioides (B8H1Z0), Caulobacter vibrioides
Manually annotated by BRENDA team
Nygard, Y.; Maaheimo, H.; Mojzita, D.; Toivari, M.; Wiebe, M.; Resnekov, O.; Gustavo Pesce, C.; Ruohonen, L.; Penttilae, M.
Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae
Metab. Eng.
25
238-247
2014
Caulobacter vibrioides (B8H1Z0), Caulobacter vibrioides NA1000 (B8H1Z0)
Manually annotated by BRENDA team
Toivari, M.; Vehkomaeki, M.; Nygard, Y.; Penttilae, M.; Ruohonen, L.; Wiebe, M.
Low pH D-xylonate production with Pichia kudriavzevii
Biores. Technol.
133
555-562
2013
Caulobacter vibrioides (B8H1Z0), Caulobacter vibrioides, Caulobacter vibrioides NA1000 (B8H1Z0)
Manually annotated by BRENDA team
Sanchez-Moreno, I.; Benito-Arenas, R.; Montero-Calle, P.; Hermida, C.; Garcia-Junceda, E.; Fernandez-Mayoralas, A.
Simple and practical multigram synthesis of D-xylonate using a recombinant xylose dehydrogenase
ACS Omega
4
10593-10598
2019
Caulobacter vibrioides
Manually annotated by BRENDA team
Lee, C.; Jordan, D.; Stoller, J.; Kibblewhite, R.; Wagschal, K.
Biochemical characterization of Caulobacter crescentus xylose dehydrogenase
Int. J. Biol. Macromol.
118
1362-1367
2018
Caulobacter vibrioides
Manually annotated by BRENDA team