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Information on EC 1.1.1.153 - sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) and Organism(s) Bos taurus and UniProt Accession Q17QK8

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EC Tree
IUBMB Comments
This enzyme catalyses the final step in the de novo synthesis of tetrahydrobiopterin from GTP. The enzyme, which is found in higher animals and some fungi and bacteria, produces the erythro form of tetrahydrobiopterin. cf. EC 1.1.1.325, sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming).
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This record set is specific for:
Bos taurus
UNIPROT: Q17QK8
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
akr1b1, sepiapterin reductase, mdspr, mdspi16 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
reductase, sepiapterin
-
-
-
-
SPR
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
L-erythro-7,8-dihydrobiopterin:NADP+ oxidoreductase
This enzyme catalyses the final step in the de novo synthesis of tetrahydrobiopterin from GTP. The enzyme, which is found in higher animals and some fungi and bacteria, produces the erythro form of tetrahydrobiopterin. cf. EC 1.1.1.325, sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming).
CAS REGISTRY NUMBER
COMMENTARY hide
9059-48-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sepiapterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
show the reaction diagram
-
-
-
?
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-(S)-lactoyl-7,8-dihydropterin + NADPH
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-acetyl-L-noradrenaline
-
weak inhibitor, N-acetyl-L-adrenaline does not bind enzyme
N-acetyl-p-tyramine
-
weak, N-acetyl-m-tyramine shows strong inhibition
N-acetyldopamine
-
potent competitive inhibitor
N-acetylphenethylamine
-
weak
N-acetylserotonin
-
potent competitive inhibitor
N-acetyltryptamine
-
slight, N-acetyltryptophan does not bind enzyme
N-chloroacetyldopamine
-
strong inhibitor
N-chloroacetylserotonin
-
strong competitive inhibitor
N-lactoyldopamine
-
potent inhibitor
N-methoxyacetylserotonin
-
strong competitive inhibitor
N-pyruvoyldopamine
-
potent inhibitor
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.76
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
adrenal medulla
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SPRE_BOVIN
267
0
28939
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29500
-
2 * 29500
59000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
2 * 29500
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
20°C, after ion exchange chromatography, no change in activity over one year of storage
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Smith, G.K.; Duch, D.S.; Edelstein, M.P.; Bigham, E.C.
New inhibitors of sepiapterin reductase
J. Biol. Chem.
267
5599-5607
1992
Bos taurus
Manually annotated by BRENDA team
Dowling, T.G.; O'Rourke, J.F.; Tipton, K.F.
Sepiapterin reductase and ALR2 ("aldose reductase") from bovine brain
Adv. Exp. Med. Biol.
328
313-324
1993
Bos taurus
Manually annotated by BRENDA team
Gao, L.; Pung, Y.F.; Zhang, J.; Chen, P.; Wang, T.; Li, M.; Meza, M.; Toro, L.; Cai, H.
Sepiapterin reductase regulation of endothelial tetrahydrobiopterin and nitric oxide bioavailability
Am. J. Physiol. Heart Circ. Physiol.
297
H331-H339
2009
Bos taurus (Q17QK8), Bos taurus
Manually annotated by BRENDA team