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Information on EC 1.1.1.148 - estradiol 17alpha-dehydrogenase for references in articles please use BRENDA:EC1.1.1.148
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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
17alpha-hydroxysteroid dehydrogenase, 17alpha-hsd, 17alpha-hydroxy steroid dehydrogenase,
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17alpha-estradiol dehydrogenase
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17alpha-hydroxy steroid dehydrogenase
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17alpha-hydroxy steroid oxidoreductase
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17alpha-hydroxysteroid dehydrogenase
17alpha-hydroxysteroid oxidoreductase
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estradiol 17alpha-oxidoreductase
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17alpha-hydroxysteroid dehydrogenase
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17alpha-hydroxysteroid dehydrogenase
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17alpha-hydroxysteroid dehydrogenase
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additional information
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N-terminal sequence comparison of several enzymes: 17alpha-hydroxysteroid dehydrogenase belongs to the disulfide oxidoreductase family, subfamily dihydrolipoamide dehydrogenases
additional information
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N-terminal sequence comparison of several enzymes: 17alpha-hydroxysteroid dehydrogenase belongs to the disulfide oxidoreductase family, subfamily dihydrolipoamide dehydrogenases
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estradiol-17alpha + NAD(P)+ = estrone + NAD(P)H + H+
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17alpha-hydroxysteroid:NAD(P)+ 17-oxidoreductase
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17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
17alpha-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
17beta-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
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testosterone
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5alpha-androstan-3beta,17alpha-diol + NADP+
3beta-hydroxy-5alpha-androstan-17-one + NADPH
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5alpha-dihydrotestosterone + NADPH
5alpha-androstane-3alpha,17alpha-diol + NADP+
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5alpha-pregnane-3,20-dione + NADPH + H+
3alpha,5alpha-3-hydroxypregnan-20-one + NADP+
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androstanedione + NADPH
5alpha-androstane-3-one-17alpha-ol + NADP+
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androstenedione + NADPH + H+
epitestosterone + NADP+
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androsterone + NADPH
5alpha-androstane-3alpha,17alpha-diol + NADP+
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dehydroepiandrosterone + NADPH
5-androstene-3alpha,17alpha-diol + NADP+
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estradiol-17alpha 3-galacturonide + NADP+
estrone 3-galacturonide + NADPH
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estradiol-17alpha 3-glucoside + NADP+
estrone 3-glucoside + NADPH
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estradiol-17alpha 3-glucuronide + NADP+
estrone 3-glucuronide + NADPH
additional information
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17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
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17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
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r
17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
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17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
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17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
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r
17alpha-estradiol + NAD(P)+
estrone + NAD(P)H
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17alpha-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
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epitestosterone
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17alpha-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
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epitestosterone
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17alpha-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
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epitestosterone
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17alpha-hydroxy-4-androsten-3-one + NADP+
4-androsten-3,17-dione + NADPH
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epitestosterone
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estradiol-17alpha 3-glucuronide + NADP+
estrone 3-glucuronide + NADPH
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estradiol-17alpha 3-glucuronide + NADP+
estrone 3-glucuronide + NADPH
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isoform IC is more specific for this substrate than for 17alpha-estradiol
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additional information
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the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
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additional information
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the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
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additional information
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substrate specificity of the recombinant enzyme, overview
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additional information
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substrate specificity of the recombinant enzyme, overview
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additional information
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additional information
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the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
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additional information
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Q91WR5
the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
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NAD+
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preferred
NADP+
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40% of NAD+ activity
NADP+
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activity 20 times greater than with NAD+
NADP+
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highly specific for
NADPH
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17beta-(1-Oxoprop-2-ynyl)androst-4-ene-3-one
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Epitestosterone
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substrate inhibition for all isoforms at concentration above 0.003 mM, not observed with other substrates
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0.0017 - 0.025
17alpha-estradiol
0.001 - 0.0051
17alpha-estradiol 3-glucuronide
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for six isoforms
0.031
4-androsten-3,17-dione
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0.0002 - 0.07
Epitestosterone
0.0017
17alpha-estradiol
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0.0024 - 0.0088
17alpha-estradiol
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for six isoforms
0.017 - 0.025
17alpha-estradiol
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0.0002 - 0.0052
Epitestosterone
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for six isoforms
0.002
NADP+
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0.003 - 0.009
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isoform IA, IB, IID, III, 17alpha-estradiol 3-glucuronide
0.007 - 0.015
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six isoforms, 17alpha-estradiol
0.041 - 0.2
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six isoforms, epitestosterone
0.047
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isoform IIC, 17alpha-estradiol 3-glucuronide
0.18
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isoform IC, 17alpha-estradiol 3-glucuronide
1.8
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oxidation of 17alpha-hydroxy-4-androsten-3-one
3.25
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reduction of 4-androsten-3,17-dione
additional information
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5.5 - 7
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reduction of 4-androsten-3,17-dione
6.5
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oxidation of 17alpha-hydroxy-4-androsten-3-one
9 - 9.5
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inducible by growth on androstenedione and cholic acid, not deoxycholic acid; VPI 12708
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VPI 12708
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SwissProt
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eight isoforms
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high expression level
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additional information
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enzyme tissue distribution in male and female mice, overview
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additional information
enzyme tissue distribution in male and female mice, overview
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AK1CL_MOUSE
323
0
36877
Swiss-Prot
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33000
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x * 33000, SDS-PAGE
39600
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x * 39600, SDS-PAGE, all 8 forms
42000
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4 * 42000, SDS-PAGE
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x * 33000, SDS-PAGE
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x * 39600, SDS-PAGE, all 8 forms
tetramer
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4 * 42000, SDS-PAGE
tetramer
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4 * 42000, SDS-PAGE
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sensitive to molecular oxygen
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-20°C, 50% v/v glycerol
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4°C, 5 mM phosphate buffer, pH 7.2, 20% v/v glycerol, at least 3 days
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4°C, phosphate buffer, pH 7.0, 1 mM dithiothreitol, at least 1 month
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8 forms, six of them purified to homogenity: IA, IB, IC, IIC, IID, III
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recombinant GST-fusion enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
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cDNA cloning, subcloning in Escherichia coli, expression as GST-fusion protein in Escherichia coli strain BL21(DE3), stable expression in HEK-293 cells
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LaRhee, L.H.; Warren, J.C.
Purification and characterization of epimeric estradiol dehydrogenases (17alpha and 17beta) from equine placenta
Biochemistry
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486-491
1984
Equus caballus
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Johnston, J.; Renwick, A.G.C.
Oestradiol-17alpha dehydrogenase from chicken liver
Biochem. J.
222
761-768
1984
Gallus gallus
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Hasnain, S.; Williamson, D.G.
Properties of the multiple forms of the soluble 17alpha-hydroxy steroid dehydrogenase of rabbit liver
Biochem. J.
161
279-283
1977
Oryctolagus cuniculus
brenda
Hasnain, S.; Williamson, D.G.
Purification of multiple forms of the soluble 17alpha-hydroxy steroid dehydrogenase or rabbit liver
Biochem. J.
147
457-461
1975
Oryctolagus cuniculus
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Renwick, A.G.C.; Engel, L.L.
The partial purification of 17alpha- and 17beta-estradiol dehydrogenase activities from chicken liver
Biochim. Biophys. Acta
146
336-348
1967
Gallus gallus
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Hasnain, S.; Williamson, D.G.
The separation and partial purification of the soluble 17alpha- and 17beta-hydroxysteroid dehydrogenases of rabbit liver
Can. J. Biochem.
52
120-125
1974
Oryctolagus cuniculus
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De Prada, P.; Setchell, K.D.R.; Hylemon, P.B.
Purification and characterization of a novel 17alpha-hydroxysteroid dehydrogenase from an intestinal Eubacterium sp. VPI 12708
J. Lipid Res.
35
922-929
1994
Eubacterium sp., Eubacterium sp. VPI 12708
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Bellemare, V.; Faucher, F.; Breton, R.; Luu-The, V.
Characterization of 17alpha-hydroxysteroid dehydrogenase activity (17alpha-HSD) and its involvement in the biosynthesis of epitestosterone
BMC Biochem.
6
12
2005
Mus musculus, Mus musculus (Q91WR5)
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1.1.1.148 estradiol 17alpha-dehydrogenase
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