Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
IUBMB CommentsThis activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
Synonyms
hsd3b2, hsd3b, dhrs4, 3-beta-hydroxysteroid dehydrogenase, hsd3b6, 3betahsd2, 3beta hydroxysteroid dehydrogenase, 3beta-hydroxysteroid dehydrogenase type ii, 3beta-hydroxysteroid dehydrogenase type 2, 3beta-hsd type ii,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3beta-hydroxysteroid dehydrogenase
-
3-beta-hydroxy-5-ene steroid dehydrogenase
-
-
-
-
3-beta-hydroxy-delta(5)-steroid dehydrogenase
-
-
-
-
3-beta-hydroxysteroid dehydrogenase
-
-
3beta-hydroxy steroid dehydrogenase/5-ene-4-ene isomerase
-
-
-
-
3beta-hydroxy steroid dehydrogenase/isomerase
-
-
-
-
3beta-hydroxy-5-ene steroid dehydrogenase
-
-
-
-
3beta-hydroxy-5-ene-steroid dehydrogenase
-
-
-
-
3beta-hydroxy-5-ene-steroid oxidoreductase
-
-
-
-
3beta-hydroxy-DELTA5-C27-steroid dehydrogenase/isomerase
-
-
-
-
3beta-hydroxy-DELTA5-C27-steroid oxidoreductase
-
-
-
-
5-ene-3-beta-hydroxysteroid dehydrogenase
-
-
-
-
5alpha-dihydrotestosterone 3beta-hydroxysteroid dehydrogenase
-
-
-
-
dehydrogenase, 3beta-hydroxy-DELTA5-steroid
-
-
-
-
DELTA5-3beta-hydroxysteroid dehydrogenase
-
-
-
-
progesterone reductase
-
-
-
-
steroid-DELTA5-3beta-ol dehydrogenase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
androstenone + NADH
beta-androstenol + NAD+
-
-
-
?
11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione + NADPH
3beta,11beta,17alpha,21-tetrahydroxy-4-pregnen-20-one + NADP+
-
-
-
-
?
11beta,17alpha,21-trihydroxy-5alpha-pregnane-3,20-dione + NADPH
3beta,11beta,17alpha,21-tetrahydroxy-5alpha-pregnan-20-one + NADP+
-
-
-
-
?
11beta,17alpha,21-trihydroxy-5beta-pregnane-3,20-dione + NADPH
3beta,11beta,17alpha,21-tetrahydroxy-5beta-pregnan-20-one + NADP+
-
-
-
-
?
13,14-dihydro-15-keto-prostaglandin F2 + NADPH
?
-
-
-
-
?
13,14-dihydro-15-keto-prostaglandin F2alpha + NADPH
?
-
-
-
-
?
17-hydroxyprogesterone + NADPH
3beta,17-dihydroxy-4-pregnen-20-one + NADP+
-
-
-
-
?
17beta-hydroxy-5alpha-androstane-3-one + NADPH
3beta,17beta-dihydroxy-5alpha-androstane + NADP+
-
-
-
-
?
17beta-hydroxy-5beta-androstane-3-one + NADPH
3beta,17beta-dihydroxy-5beta-androstane + NADP+
-
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
-
-
-
?
3beta,7alpha-dihydroxy-5-cholestenoic acid + NAD+
7alpha-hydroxy-3-oxo-5-cholestenoic acid + NADH
-
-
-
-
?
4-benzylpyridine + NADPH
?
-
-
-
-
?
4-carboxybenzaldehyde + NADP+ + H2O
4-carboxybenzoic acid + NADPH + H+
-
-
-
-
?
4-nitroacetophenone + NADPH
1-(4-nitrophenyl)ethanol + NADP+
-
-
-
-
?
4-nitrobenzaldehyde + NADPH
4-nitrobenzyl alcohol + NADP+
-
-
-
-
?
5,16-androstadien-3beta-ol + NAD+
5,16-androstadien-3-one + NADH
-
-
-
-
?
5alpha-androstane-3,17-dione + NADPH
3beta-hydroxy-5alpha-androstane-17-one + NADP+
-
-
-
-
?
5alpha-dihydrotestosterone + NADPH
5alpha-androstane-3beta,17beta-diol + NADP+
-
-
-
-
?
5alpha-pregnane-3,20-dione + NAD(P)H
5alpha-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
?
5beta-androstane-3,17-dione + NADPH
3beta-hydroxy-5beta-androstane-17-one + NADP+
-
-
-
-
?
5beta-pregnane-3,20-dione + NAD(P)H + H+
5beta-pregnane-3beta-ol-20-one + NAD(P)+
-
-
-
-
?
7alpha,25-dihydroxycholesterol + NAD+
7alpha,25-dihydroxy-4-cholesten-3-one + NADH
-
-
-
-
?
7alpha,27-dihydroxycholesterol + NAD+
7alpha,27-dihydroxy-4-cholesten-3-one + NADH
-
-
-
-
?
7alpha-hydroxycholesterol + NAD+
7alpha-hydroxy-4-cholesten-3-one + NADH
-
-
-
-
?
9,10-phenanthrenequinone + NADPH
?
-
-
-
-
?
9,10-phenanthrenequinone + NADPH
? + NADP+
-
-
-
-
?
androstenedione + NADPH + H+
3beta-hydroxy-5-androsten-17-one + NADP+
-
-
-
-
?
androstenone + NAD+
5alpha-androst-16-en-3beta-ol + NADH + H+
-
-
-
-
?
camphorquinone + NADPH + H+
camphorquinol + NADP+
-
-
-
-
?
caprinaldehyde + NADPH
?
-
-
-
-
?
cyclohexanone + NADPH
cyclohexanol + NADP+
-
-
-
-
?
D-galactose + NADPH
?
-
-
-
-
?
D-glucose + NADPH
?
-
-
-
-
?
D-glucuronic acid + NADPH
?
-
-
-
-
?
D-glyceraldehyde + NADPH
?
-
-
-
-
?
D-xylose + NADPH
?
-
-
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH + H+
-
-
-
-
?
dehydroepiandrosterone + NADPH
3beta-hydroxy-5-androsten-17-one + NADP+
-
-
-
-
?
hydritantin + NADPH
?
-
-
-
-
?
menadione + NADPH
menadiol + NADP+
-
-
-
-
?
methylglyoxal + NADPH
?
-
-
-
-
?
phenylglyoxal + NADPH
?
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H + H+
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
-
-
-
-
?
progesterone + NADPH
3beta-hydroxy-4-pregnen-20-one + NADP+
-
-
-
-
?
prostaglandin E1 + NADPH
?
-
-
-
-
?
prostaglandin E2 + NADPH
?
-
-
-
-
?
testosterone + NADPH + H+
3beta,17beta-dihydroxy-4-androsten + NADP+
-
-
-
-
?
additional information
?
-
additional information
?
-
relationship between the expression of hepatic but not testicular enzyme with androstenone deposition in pig adipose tissue, overview
-
-
?
additional information
?
-
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0038
11beta,17alpha,21-trihydroxy-4-pregnene-3,20-dione
-
-
0.0827
11beta,17alpha,21-trihydroxy-5alpha-pregnane-3,20-dione
-
-
0.0461
11beta,17alpha,21-trihydroxy-5beta-pregnane-3,20-dione
-
-
0.0621
13,14-dihydro-15-keto-prostaglandin F2
-
-
0.13
13,14-dihydro-15-keto-prostaglandin F2alpha
-
-
0.206
17-hydroxyprogesterone
-
-
0.684
17beta-hydroxy-5alpha-androstane-3-one
-
-
0.231
17beta-hydroxy-5beta-androstane-3-one
-
-
2.097
4-benzylpyridine
-
-
0.319
4-carboxybenzaldehyde
-
-
0.137
4-Nitroacetophenone
-
-
1.966
4-nitrobenzaldehyde
-
-
1.043
5alpha-androstane-3,17-dione
-
-
0.217
5alpha-pregnane-3,20-dione
-
-
0.123
5beta-androstane-3,17-dione
-
-
0.246
5beta-pregnane-3,20-dione
-
-
0.003
7alpha,27-dihydroxycholesterol
-
-
0.0032
7alpha-hydroxycholesterol
-
-
2.408
9,10-phenanthrenequinone
-
-
0.0703
androstenedione
-
-
0.465
D-glucuronic acid
-
-
1.07
D-glyceraldehyde
-
-
0.114
dehydroepiandrosterone
-
-
0.373
prostaglandin E1
-
-
0.0152
prostaglandin E2
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Furster, C.; Zhang, J.; Toll, A.
Purification of a 3beta-hydroxy-D5-C27-steroid dehydrogenase from pig liver microsomes active in major and alternative pathways of bile acid biosynthesis
J. Biol. Chem.
271
20903-20907
1996
Sus scrofa
brenda
Hebert, P.; Cooke, G.M.
Kinetic evidence for separate 3beta-hydroxysteroid dehydrogenase-isomerases in androgen and 16-androstene biosynthetic pathways in the pig testis
J. Steroid Biochem. Mol. Biol.
42
901-910
1992
Sus scrofa
brenda
Nakajin, S.; Ishii, A.; Shinoda, M.
Purification and characterization of 5alpha-dihydrotestosterone 3beta-hydroxysteroid dehydrogenase from mature pig testicular cytosol
Biol. Pharm. Bull.
17
1155-1160
1994
Sus scrofa
brenda
Nicolau-Solano, S.I.; McGivan, J.D.; Whittington, F.M.; Nieuwhof, G.J.; Wood, J.D.; Doran, O.
Relationship between the expression of hepatic but not testicular 3beta-hydroxysteroid dehydrogenase with androstenone deposition in pig adipose tissue
J. Anim. Sci.
84
2809-2817
2006
Sus scrofa (Q9N119)
brenda
Nicolau-Solano, S.I.; Whittington, F.M.; Wood, J.D.; Doran, O.
Relationship between carcass weight, adipose tissue androstenone level and expression of the hepatic 3beta -hydroxysteroid dehydrogenase in entire commercial pigs
Animal
1
1053-1059
2007
Sus scrofa
brenda
Knapczyk, K.; Duda, M.; Czaplicki, D.; Slomczynska, M.
The presence of 3beta-hydroxysteroid dehydrogenase in the ovarian follicles and corpora lutea of pregnant swine
Exp. Clin. Endocrinol. Diabetes
115
411-416
2007
Sus scrofa
brenda
Pawlak, K.J.; Prasad, M.; Thomas, J.L.; Whittal, R.M.; Bose, H.S.
Inner mitochondrial translocase Tim50 interacts with 3beta-hydroxysteroid dehydrogenase type 2 to regulate adrenal and gonadal steroidogenesis
J. Biol. Chem.
286
39130-39140
2011
Sus scrofa
brenda
Wojciechowicz, B.; Kotwica, G.; Kolakowska, J.; Franczak, A.
The activity and localization of 3beta-hydroxysteroid dehydrogenase/DELTA(5)-DELTA(4) isomerase and release of androstenedione and progesterone by uterine tissues during early pregnancy and the estrous cycle in pigs
J. Reprod. Dev.
59
49-58
2013
Sus scrofa
brenda
Rasmussen, M.; Brunius, C.; Ekstrand, B.; Zamaratskaia, G.
Expression of hepatic 3beta-hydroxysteroid dehydrogenase and sulfotransferase 2A1 in entire and castrated male pigs
Mol. Biol. Rep.
39
7927-32
2012
Sus scrofa (Q9N119)
brenda
Smolinska, N.; Dobrzyn, K.; Kiezun, M.; Szeszko, K.; Maleszka, A.; Kaminski, T.
Effect of adiponectin on the steroidogenic acute regulatory protein, P450 side chain cleavage enzyme and 3beta-hydroxysteroid dehydrogenase gene expression, progesterone and androstenedione production by the porcine uterus during early pregnancy
J. Physiol. Pharmacol.
67
443-456
2016
Sus scrofa
brenda
Chen, G.; Bai, Y.; Ren, L.; Zhu, D.; Li, Y.; Fang, M.; Al-Kateb, H.; Doran, O.
Metabolism of androstenone, 17beta-estradiol and dihydrotestosterone in primary cultured pig hepatocytes and the role of 3beta-hydroxysteroid dehydrogenase in this process
PLoS ONE
10
e113194
2015
Sus scrofa
brenda