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Information on EC 1.1.1.145 - 3beta-hydroxy-DELTA5-steroid dehydrogenase and Organism(s) Rattus norvegicus and UniProt Accession P22071

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IUBMB Comments
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
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Rattus norvegicus
UNIPROT: P22071
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The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
hsd3b2, hsd3b, dhrs4, 3-beta-hydroxysteroid dehydrogenase, hsd3b6, 3betahsd2, 3beta hydroxysteroid dehydrogenase, 3beta-hydroxysteroid dehydrogenase type ii, 3beta-hydroxysteroid dehydrogenase type 2, 3beta-hsd type ii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3beta hydroxysteroid dehydrogenase
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3-beta-hydroxy-5-ene steroid dehydrogenase
-
-
-
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3-beta-hydroxy-delta(5)-steroid dehydrogenase
-
-
-
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3beta-HSDH
-
-
-
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3beta-hydroxy steroid dehydrogenase/5-ene-4-ene isomerase
-
-
-
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3beta-hydroxy steroid dehydrogenase/isomerase
-
-
-
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3beta-hydroxy-5-ene steroid dehydrogenase
-
-
-
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3beta-hydroxy-5-ene-steroid dehydrogenase
-
-
-
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3beta-hydroxy-5-ene-steroid oxidoreductase
-
-
-
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3beta-hydroxy-DELTA5-C27-steroid dehydrogenase/isomerase
-
-
-
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3beta-hydroxy-DELTA5-C27-steroid oxidoreductase
-
-
-
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3beta-hydroxysteroid dehydrogenase
-
-
3beta-hydroxysteroid dehydrogenase/isomerase
-
-
5-ene-3-beta-hydroxysteroid dehydrogenase
-
-
-
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5alpha-dihydrotestosterone 3beta-hydroxysteroid dehydrogenase
-
-
-
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dehydrogenase, 3beta-hydroxy-DELTA5-steroid
-
-
-
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DELTA5-3beta-hydroxysteroid dehydrogenase
-
-
-
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progesterone reductase
-
-
-
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steroid-DELTA5-3beta-ol dehydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
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oxidation
-
-
-
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reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3beta-hydroxy-DELTA5-steroid:NAD+ 3-oxidoreductase
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
CAS REGISTRY NUMBER
COMMENTARY hide
9044-85-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pregnenolone + NAD+
progesterone + NADH + H+
show the reaction diagram
-
-
-
?
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
show the reaction diagram
-
-
-
-
?
5-pregnen-3beta-ol-2-one + NAD+
progesterone + NADH
show the reaction diagram
-
trivial name pregnenolone
-
-
?
dehydroepiandrosterone + NAD+
?
show the reaction diagram
-
-
-
-
r
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH + H+
show the reaction diagram
-
-
-
-
?
dehydroepiandrosterone + NADH + H+
androsterone + NAD+
show the reaction diagram
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
show the reaction diagram
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-pregnen-3beta-ol-2-one + NAD+
progesterone + NADH
show the reaction diagram
-
trivial name pregnenolone
-
-
?
dehydroepiandrosterone + NAD+
androst-5-en-3,17-dione + NADH + H+
show the reaction diagram
-
-
-
-
?
dehydroepiandrosterone + NADH + H+
androsterone + NAD+
show the reaction diagram
-
-
-
-
?
pregnenolone + NAD(P)+
pregn-5-en-3,20-dione + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
pregnenolone + NAD+
pregn-5-en-3,20-dione + NADH + H+
show the reaction diagram
-
-
-
-
?
pregnenolone + NAD+
progesterone + NADH + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme regulation by steroids, overview
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+)-gossypol
-
potent inhibitor
(-)-gossypol
-
potent inhibitor
4-nonylphenol
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
5'-fluorosulfonylbenzoyl adenosine
-
-
corticosterone
-
0.08 mM, 50% inhibition
diethylhexyl phthalate
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
diethylstilbestrol
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
estradiol
-
0.0005 mM, 40% inhibition, 0.001 mM, 62% inhibition
ethynylestradiol
-
-
ketoconazole
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
monooctyltin
Norethisterone
-
-
Norethisterone acetate
-
-
octylphenol
-
downregulation of 3beta-hydroxysteroid dehydrogenase mRNA at all doses tested
oestradiol
-
0.00024 mM, 50% inhibition
progesterone
testosterone
-
0.0086 mM, 50% inhibition
tributyltin
trilostane
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flutamide
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increase in mRNA expression in testis
testosterone
-
at low dose, increase in mRNA expression. At doses above 0.2 mg/kg/day, downregulation of 3beta-hydroxysteroid dehydrogenase mRNA
additional information
3beta-HSD mRNA level significantly increases in Leydig cells of corticosterone-deficient rats and simultaneous corticosterone treatment completely maintains the expression at control levels
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00009 - 0.005
dehydroepiandrosterone
0.0000047 - 0.045
NAD+
13
NADP+
-
-
0.00013 - 0.0011
pregnenolone
additional information
additional information
-
kinetic analysis
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0027
tributyltin
additional information
additional information
-
inhibition kinetics
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00019
(+)-gossypol
Rattus norvegicus
-
in PBS buffer, pH 7.2
0.00023
(-)-gossypol
Rattus norvegicus
-
in PBS buffer, pH 7.2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 12
-
approx. 50% of maximal activity at pH 4.0 and pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 65
-
approx. 50% of maximal activity at 20°C and 45°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
twenty-four hours after bilateral contusion of the medial prefrontal cortex, similar levels of 3beta-hydroxysteroid dehydrogenase mRNA expression are observed in males and pseudopregnant females in the non-injured group, with a significant decrease in the 3beta-hydroxysteroid dehydrogenase mRNA expression in the contusion site within the frontal cortex in both males and pseudopregnant females. In all other regions analyzed, 3beta-hydroxysteroid dehydrogenase mRNA expression is not affected by traumatic brain injury and there is no difference between males and pseudopregnant females
Manually annotated by BRENDA team
-
weak expression of 3beta-hydroxysteroid dehydrogenase in newly formed corpora lutea, which gradually increases followed by decrease on day 20. In vascularised apical region of young corpora lutea, luteal cells with more intensive immunostaining predominate. Distribution of 3beta-hydroxysteroid dehydrogenase and androgen receptor differ within various generations of corpora lutea
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
inner mitochondrial membrane
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
3BHS1_RAT
373
0
42037
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
x * 45000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression analysis of the enzyme in sciatic nerves
-
expression in COS-1 monkey kidney tumor cells
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transient expression of HSD I and II cDNA in HeLa cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
twenty-four hours after bilateral contusion of the medial prefrontal cortex, similar levels of 3beta-hydroxysteroid dehydrogenase mRNA expression are observed in males and pseudopregnant females in the non-injured group, with a significant decrease in the 3beta-hydroxysteroid dehydrogenase mRNA expression in the contusion site within the frontal cortex in both males and pseudopregnant females. In all other regions analyzed, 3beta-hydroxysteroid dehydrogenase mRNA expression is not affected by traumatic brain injury and there is no difference between males and pseudopregnant females
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Koritz, S.B.
The conversion of pregnenolone to progesterone by small particles from rat adrenal
Biochemistry
3
1098-1102
1964
Rattus norvegicus
Manually annotated by BRENDA team
Ishii-Ohba, H.; Inano, H.; Bunichi, T.
Testicular and adrenal 3beta-hydroxy-5-ene-steroid dehydrogenase and 5-ene-4-ene isomerase
J. Steroid Biochem.
27
775-779
1987
Rattus norvegicus
Manually annotated by BRENDA team
Ishii-Ohba, H.; Inano, H.; Tamaoki, B.
Purification and properties of testicular 3beta-hydroxy-5-ene-steroid dehydrogenase and 5-ene-4-ene isomerase
J. Steroid Biochem.
25
555-560
1986
Rattus norvegicus
Manually annotated by BRENDA team
Spona, J.
Differences in inhibition by various steroids of rat testis and Pseudomonas testosteroni DELTA5-3beta-hydroxysteroid dehydrogenase
Endocrinol. Exp.
17
107-118
1983
Comamonas testosteroni, Rattus norvegicus
Manually annotated by BRENDA team
Labrie, F.; Simard, J.; Luu-The, V.; Pelletier, G.; Belanger, A.; Lachance, Y.; Zhao, H.F.; Labrie, C.; Breton, N.; de Launoit, Y.; et al.
Structure and tissue-specific expression of 3beta-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase genes in human and rat classical and peripheral steroidogenic tissues
J. Steroid Biochem. Mol. Biol.
41
421-435
1992
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Lorence, M.C.; Naville, D.; Graham-Lorence, S.E.; Mack, S.O.; Murry, B.A.; Trant, J.M.; Mason, J.I.
3beta-Hydroxysteroid dehydrogenase/DELTA 5-4-isomerase expression in rat and characterization of the testis isoform
Mol. Cell. Endocrinol.
80
21-31
1991
Rattus norvegicus
Manually annotated by BRENDA team
Perry, J.E.; Ishii-Ohba, H.; Stalvey, J.R.
Subcellular distribution of 3beta-hydroxysteroid dehydrogenase-isomerase in bovine and murine adrenocortical tissue: species differences in the localization of activity and immunoreactivity
Steroids
56
329-336
1991
Bos taurus, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Coirini, H.; Gouezou, M.; Delespierre, B.; Liere, P.; Pianos, A.; Eychenne, B.; Schumacher, M.; Guennoun, R.
Characterization and regulation of the 3beta-hydroxysteroid dehydrogenase isomerase enzyme in the rat sciatic nerve
J. Neurochem.
84
119-126
2003
Rattus norvegicus
Manually annotated by BRENDA team
Coirini, H.; Gouezou, M.; Delespierre, B.; Schumacher, M.; Guennoun, R.
3 beta-hydroxysteroid dehydrogenase isomerase (3beta-HSD) activity in the rat sciatic nerve: kinetic analysis and regulation by steroids
J. Steroid Biochem. Mol. Biol.
85
89-94
2003
Rattus norvegicus
Manually annotated by BRENDA team
McVey, M.J.; Cooke, G.M.
Inhibition of rat testis microsomal 3b-hydroxysteroid dehydrogenase activity by tributyltin
J. Steroid Biochem. Mol. Biol.
86
99-105
2003
Rattus norvegicus
Manually annotated by BRENDA team
Qujeq, D.
Development of a quantitative assay method for 3 beta-hydroxy-DELTA 5-steroid dehydrogenase in the rat testis
Steroids
67
1071-1077
2002
Rattus norvegicus
Manually annotated by BRENDA team
Meffre, D.; Delespierre, B.; Gouezou, M.; Schumacher, M.; Stein, D.G.; Guennoun, R.
3beta-Hydroxysteroid dehydrogenase/5-ene-4-ene isomerase mRNA expression in rat brain: effect of pseudopregnancy and traumatic brain injury
J. Steroid Biochem. Mol. Biol.
104
293-300
2007
Rattus norvegicus (P22071)
Manually annotated by BRENDA team
Kim, H.H.; Kwak, D.H.; Yon, J.M.; Baek, I.J.; Lee, S.R.; Lee, J.E.; Nahm, S.S.; Jeong, J.H.; Lee, B.J.; Yun, Y.W.; Nam, S.Y.
Differential expression of 3beta-hydroxysteroid dehydrogenase mRNA in rat testes exposed to endocrine disruptors
J. Reprod. Dev.
53
465-471
2007
Rattus norvegicus
Manually annotated by BRENDA team
Malouitre, S.D.; Barker, S.; Puddefoot, J.R.; Jalili, J.; Glover, H.R.; Vinson, G.P.
Regulation of hepatic steroid receptors and enzymes by the 3beta-hydroxysteroid dehydrogenase inhibitor trilostane
J. Steroid Biochem. Mol. Biol.
101
97-105
2006
Rattus norvegicus
Manually annotated by BRENDA team
Szoltys, M.; Slomczynska, M.; Galas, J.; Duda, M.; Sakiewicz, A.
Expression of androgen receptor and 3beta-hydroxysteroid dehydrogenase in corpora lutea during pregnancy in the rat
Reprod. Fertil. Dev.
19
356-365
2007
Rattus norvegicus
Manually annotated by BRENDA team
Parthasarathy, C.; Balasubramanian, K.
Effects of corticosterone deficiency and its replacement on Leydig cell steroidogenesis
J. Cell. Biochem.
104
1671-1683
2008
Rattus norvegicus (P22071)
Manually annotated by BRENDA team
Hu, G.X.; Zhou, H.Y.; Li, X.W.; Chen, B.B.; Xiao, Y.C.; Lian, Q.Q.; Liang, G.; Kim, H.H.; Zheng, Z.Q.; Hardy, D.O.; Ge, R.S.
The (+)- and (-)-gossypols potently inhibit both 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase 3 in human and rat testes
J. Steroid Biochem. Mol. Biol.
115
14-19
2009
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Ryzhavskii, B.Y.; Zadvornaya, O.V.
3beta-hydroxysteroid dehydrogenase in the brain ependymocytes
Bull. Exp. Biol. Med.
155
113-114
2013
Rattus norvegicus
Manually annotated by BRENDA team