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Information on EC 1.1.1.138 - mannitol 2-dehydrogenase (NADP+)
for references in articles please use BRENDA:EC1.1.1.138
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EC Tree 1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.1.1 With NAD+ or NADP+ as acceptor
1.1.1.138 mannitol 2-dehydrogenase (NADP+)
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 1.1.1.138
- spindle
-
checkpoint
- d-fructose
- kinetochore
-
anaphase
- cladosporium
- bub3
-
kinetochore-microtubule
-
1.1.1.67
- herbarum
- l-xylulose
- d-arabinitol
- gluconobacter
- jecorina
- mad3
-
ige-binding
- hypocrea
-
unattached
- synthesis
- analysis
- biotechnology
showSynonyms
mad1p, d-mannitol dehydrogenase, cla h 8, psldr, gox1432, nadp-dependent mannitol dehydrogenase, d-mannitol 2-dehydrogenase, tbmdh, lrmdh, nadp-mannitol dehydrogenase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D-mannitol 2-dehydrogenase
D-mannitol dehydrogenase, NADP-dependent
LXR1
mannitol 2-dehydrogenase
Mannitol 2-dehydrogenase [NADP+]
-
-
-
-
mannitol dehydrogenase
mannitol dehydrogenase (NADP+)
-
-
-
-
mannitol dehydrogenase (nicotinamide dinucleotide phosphate)
-
-
-
-
MDH
MtDH
NADP-dependent mannitol dehydrogenase
additional information
the enzyme defines a subfamily within the polyol-specific medium chain dehydrogenases
mannitol dehydrogenase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-mannitol + NADP+ = D-fructose + NADPH + H+
-
-
-
-
D-mannitol + NADP+ = D-fructose + NADPH + H+
sequential ordered bi-bi mechanism
-
D-mannitol + NADP+ = D-fructose + NADPH + H+
sequential ordered bi bi mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
D-mannitol:NADP+ 2-oxidoreductase
-
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CAS REGISTRY NUMBER
COMMENTARY
37250-68-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-deoxy-6-methyl-D-glucitol + NADP+
1-deoxy-6-methyl-D-glucose + NADPH
-
Substrates: poorer substrate than D-glucitol
Products: -
Products: -
?
1-deoxy-6-methyl-D-mannitol + NADP+
1-deoxy-6-methyl-D-fructose + NADPH
-
Substrates: poorer substrate than D-mannitol
Products: -
Products: -
?
1-deoxy-D-glucitol + NADP+
1-deoxy-D-glucose + NADPH
-
Substrates: poorer substrate than glucitol
Products: -
Products: -
?
1-deoxy-D-mannitol + NADP+
1-deoxy-D-fructose + NADPH
-
Substrates: poorer substrate than D-mannitol
Products: -
Products: -
?
arabinose + NADPH + H+
arabinitol + NADP+
-
Substrates: -
Products: -
Products: -
r
D-glucitol + NADP+
D-glucose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + 3-acetylpyridine adenine dinucleotide phosphate
D-fructose + 3-acetylpyridine adenine dinucleotide phosphate(H)
-
Substrates: 7.5% of the activity compared to NADP+
Products: -
Products: -
?
D-mannitol + thio-NADP+
D-fructose + thio-NADPH
-
Substrates: 30% of the activity compared to NADP+
Products: -
Products: -
?
D-mannose + NADPH + H+
D-mannitol + NADP+
-
Substrates: -
Products: -
Products: -
r
L-sorbitol + NADP+
L-sorbose + NADPH + H+
-
Substrates: 21% of the activity with D-mannitol
Products: -
Products: -
r
perseitol + NADP+
? + NADPH
-
Substrates: 6% of the activity compared to D-mannitol
Products: -
Products: -
?
D-arabinitol + NADP+
D-xylulose + NADPH + H+
-
Substrates: 3% of the activity compared to D-mannitol
Products: -
Products: -
?
D-arabinitol + NADP+
D-xylulose + NADPH + H+
-
Substrates: substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5
Products: -
Products: -
?
D-arabinitol + NADP+
D-xylulose + NADPH + H+
Diplodia viticola Desm.
-
Substrates: -
Products: -
Products: -
r
D-fructose + NADPH
D-mannitol + NADP+
-
Substrates: -
Products: -
Products: -
r
D-fructose + NADPH
D-mannitol + NADP+
-
Substrates: -
Products: -
Products: -
?
D-fructose + NADPH
D-mannitol + NADP+
-
Substrates: -
Products: -
Products: -
r
D-fructose + NADPH
D-mannitol + NADP+
-
Substrates: -
Products: -
Products: -
r
D-fructose + NADPH + H+
D-mannitol + NADP+
Substrates: -
Products: -
Products: -
r
D-fructose + NADPH + H+
D-mannitol + NADP+
Substrates: -
Products: -
Products: -
r
D-mannitol + NAD(P)+
D-fructose + NAD(P)H + H+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NAD(P)+
D-fructose + NAD(P)H + H+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NAD(P)+
D-fructose + NAD(P)H + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NAD+
D-fructose + NADH + H+
-
Substrates: less than 0.05% of the activity compared to NADP+
Products: -
Products: -
?
D-mannitol + NAD+
D-fructose + NADH + H+
-
Substrates: less than 25% of the activity with NADP+
Products: -
Products: -
r
D-mannitol + NAD+
D-fructose + NADH + H+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: mannitol serves as storage carbon, plays a critical role in growth, fruit body development, osmoregulation and salt tolerance
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
Cenococcum graniforme
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
Ceratocystis multiannulata
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
Diplodia viticola Desm.
-
Substrates: more specific for D-mannitol than for sorbitol or D-arabinitol
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
Diplodia viticola Desm.
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: only the mutant enzymes D69A amd E68K, the wild-type enzyme utilizes NAD(H) as cofactor, EC 1.1.1.67
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: less than 3% the rate of fructose reduction
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
Starmerella magnoliae HH-01
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
Substrates: enzyme regulation mode, overview
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: the enzyme has a dual role in mannitol metabolism, D-mannitol is the main storage sugar in spores
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: the reverse reaction occurs only when D-fructose is provided as substrate, and is preferred
Products: -
Products: -
r
D-sorbitol + NADP+
D-sorbose + NADPH + H+
-
Substrates: 21% of the activity compared to D-mannitol
Products: -
Products: -
?
D-sorbitol + NADP+
D-sorbose + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
L-arabinitol + NADP+
L-xylulose + NADPH + H+
-
Substrates: 2% of the activity compared to D-mannitol
Products: -
Products: -
?
L-arabinitol + NADP+
L-xylulose + NADPH + H+
-
Substrates: 5% of the activity compared to D-mannitol
Products: -
Products: -
?
L-sorbitol + NADP+
L-sorbose + NADPH
-
Substrates: 7.7% of the activity compared to D-mannitol
Products: -
Products: -
?
L-sorbitol + NADP+
L-sorbose + NADPH
-
Substrates: substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5
Products: -
Products: -
r
L-sorbitol + NADP+
L-sorbose + NADPH
-
Substrates: 4% of the activity compared to D-mannitol
Products: -
Products: -
r
L-sorbitol + NADP+
L-sorbose + NADPH
-
Substrates: 4% of the activity compared to D-mannitol
Products: -
Products: -
r
L-sorbitol + NADP+
L-sorbose + NADPH
-
Substrates: 4% of the activity compared to D-mannitol
Products: -
Products: -
r
additional information
?
-
Substrates: the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is able to reduce D-fructose, L-sorbose, and 5-keto D-gluconate in the presence of NADPH as an electron donor
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: glucose, xylose, arabinose
Products: -
Products: -
?
additional information
?
-
-
Substrates: specific for transferring the 4-pro-S hydrogen from NAD(P)H
Products: -
Products: -
?
additional information
?
-
-
Substrates: specific for transfer of 4-pro-S hydrogen of NADPH
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme has a high preference only for D-fructose and D-mannitol, while D-mannose, D-arabinose, D-ribose, D-glucose, D-galactose, D-xylose, D-sorbose and D-sorbitol show only poor activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme has a high preference only for D-fructose and D-mannitol, while D-mannose, D-arabinose, D-ribose, D-glucose, D-galactose, D-xylose, D-sorbose and D-sorbitol show only poor activity
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-mannitol + NAD(P)+
D-fructose + NAD(P)H + H+
-
Substrates: -
Products: -
Products: -
r
D-fructose + NADPH
D-mannitol + NADP+
-
Substrates: -
Products: -
Products: -
r
D-fructose + NADPH
D-mannitol + NADP+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: mannitol serves as storage carbon, plays a critical role in growth, fruit body development, osmoregulation and salt tolerance
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: substrate must have a primary hydroxyl group at C1, an R hydroxyl at C2, an R hydroxyl at C3, an R hydroxyl at C4 and either an R or S or primary hydroxyl at C5
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
Cenococcum graniforme
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
Ceratocystis multiannulata
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
Diplodia viticola Desm.
-
Substrates: more specific for D-mannitol than for sorbitol or D-arabinitol
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
Diplodia viticola Desm.
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
Starmerella magnoliae HH-01
-
Substrates: -
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: involved in the mannitol cycle, important for NADPH regeneration
Products: -
Products: -
?
D-mannitol + NADP+
D-fructose + NADPH + H+
Substrates: enzyme regulation mode, overview
Products: -
Products: -
r
D-mannitol + NADP+
D-fructose + NADPH + H+
-
Substrates: the enzyme has a dual role in mannitol metabolism, D-mannitol is the main storage sugar in spores
Products: -
Products: -
r
additional information
?
-
Substrates: the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD(P)+
-
NADP+ is the preferred cofactor in the forward reaction
NAD(P)H
-
NADPH is the preferred cofactor in the reverse reaction
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
52% activation at 5 mM up to 140% at 10 mM only in D-mannitol oxidation
Cu2+
-
increases the intracellular activity of mannitol dehydrogenase 1.6fold
Mg2+
Zn2+
additional information
additional information
-
no stimulation by: MgCl2, MnCl2, ZnCl2, NiCl2, CoCl2, FeCl2
additional information
-
Ca2+ reduces the intracellular mannitol concetration by 30% probably by altering the permeability of cells for mannito
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KCl
-
half Vmax/Km at less than 0.1 M due to general ionic strength effect
KNO3
-
half Vmax/Km at less than 0.1 M due to general ionic strength effect
L-cysteine