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Information on EC 1.1.1.133 - dTDP-4-dehydrorhamnose reductase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LQ04

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IUBMB Comments
In the reverse direction, reduction on the 4-position of the hexose moiety takes place only while the substrate is bound to another enzyme that catalyses epimerization at C-3 and C-5; the complex has been referred to as dTDP-L-rhamnose synthase.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9LQ04
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
4-ketoreductase, agl14, dtdp-4-dehydrorhamnose reductase, dtdp-6-deoxy-l-lyxo-4-hexulose reductase, dtdp-4-keto-l-rhamnose reductase, dtdp-4-keto-rhamnose reductase, thymidine diphosphate-4-dehydrorhamnose reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dTDP-4-keto-L-rhamnose reductase
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dTDP-4-ketorhamnose reductase
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dTDP-6-deoxy-L-mannose dehydrogenase
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dTDP-L-rhamnose synthetase
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TDP-4-keto-rhamnose reductase
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TDP-4-ketorhamnose reductase
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thymidine diphospho-4-ketorhamnose reductase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
dTDP-6-deoxy-beta-L-mannose:NADP+ 4-oxidoreductase
In the reverse direction, reduction on the 4-position of the hexose moiety takes place only while the substrate is bound to another enzyme that catalyses epimerization at C-3 and C-5; the complex has been referred to as dTDP-L-rhamnose synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-64-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
dTDP-beta-L-rhamnose + NAD+
show the reaction diagram
40% of the rate with NADPH
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?
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
dTDP-beta-L-rhamnose + NADP+
show the reaction diagram
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-
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?
UDP-4-dehydro-6-deoxy-D-glucose + NADPH
UDP-beta-L-rhamnose + NADP+
show the reaction diagram
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?
additional information
?
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no substrate: GDP-4-keto-6-deoxy-mannose, UDP-glucose, dTDP-glucose, UDP-xylose, GDP-fucose
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
not inhibitory: MgCl2, CaCl2, EDTA at 10mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0169
dTDP-4-keto-6-deoxy-D-glucose
pH 8.5, 30°C
0.009
NADPH
pH 8.5, 30°C
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
about 35% of maximum activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional 3,5-epimerase/4-keto reductase
Swissprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
present in all tissues examined
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RMLCD_ARATH
301
0
33597
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
35500
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, repeated freezing and thawing, loss of more than 60% of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Watt, G.; Leoff, C.; Harper, A.D.; Bar-Peled, M.
A bifunctional 3,5-epimerase/4-keto reductase for nucleotide-rhamnose synthesis in Arabidopsis
Plant Physiol.
134
1337-1346
2004
Arabidopsis thaliana (Q9LQ04)
Manually annotated by BRENDA team