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Information on EC 1.1.1.12 - L-arabinitol 4-dehydrogenase and Organism(s) Aspergillus niger and UniProt Accession A2QAC0

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This record set is specific for:
Aspergillus niger
UNIPROT: A2QAC0 not found.
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Word Map
The taxonomic range for the selected organisms is: Aspergillus niger
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
l-arabitol dehydrogenase, l-arabinitol 4-dehydrogenase, hjlad, l-arabinitol dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L-arabitol dehydrogenase
-
dehydrogenase, L-arabinitol
-
-
-
-
L-arabitol dehydrogenase
-
-
-
-
pentitol-DPN dehydrogenase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
L-arabinitol:NAD+ 4-oxidoreductase (L-xylulose-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9028-19-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-sorbitol + NAD+
? + NADH + H+
show the reaction diagram
wild-type shows no activity on D-sorbitol, whereas mutant Y318F does
-
-
?
D-sorbitol + NAD+
D-fructose + NADH + H+
show the reaction diagram
-
-
-
?
L-arabinitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
-
-
-
?
L-arabitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
-
-
-
?
xylitol + NAD+
D-xylulose + NADH + H+
show the reaction diagram
-
-
-
?
L-arabinitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-arabinitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
no activity with NADP+
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.868 - 4.868
D-sorbitol
0.056 - 25
L-arabinitol
0.056 - 0.078
L-arabitol
0.2
NAD+
pH 7.0, 22°C
0.218 - 0.25
xylitol
8 - 9
L-arabinitol
-
30°C, pH 9.6
5
L-xylulose
-
30°C, pH 9.6
0.05
NAD+
-
30°C, pH 9.6
0.008
NADH
-
30°C, pH 9.6
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.48 - 833
D-sorbitol
8.45 - 30
L-arabinitol
863 - 1800
L-arabitol
8.23
NAD+
pH 7.0, 22°C
19.67 - 2208
xylitol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.338
L-arabinitol
pH 7.0, 22°C
41.17
NAD+
pH 7.0, 22°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.7
pH 7.0, 22°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
modelling of LadA on human SDH identifies residues M70 and Y318 in LadA, that may explain the absence of activity on D-sorbitol
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D213S/I214R
significantly higher preference for NADP+ over NAD+
Y318F
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, stable during storage for at least 2 years
-
4°C, stable during storage for months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant proteins purified using Ni-agarose
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
derivatives of the expression vector pQE32 containing wild-type and mutated versions of ladA transformed to Escherichia coli M13 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Witteveen, C.F.B.; Busink, R.; Van de Vondervoort, P.; Dijkema, C.; Swart, K.; Visser, J.
L-Arabinose and D-xylose catabolism in Aspergillus niger
J. Gen. Microbiol.
135
2163-2171
1989
Aspergillus niger
-
Manually annotated by BRENDA team
de Groot, M.J.; Prathumpai, W.; Visser, J.; Ruijter, G.J.
Metabolic control analysis of Aspergillus niger L-arabinose catabolism
Biotechnol. Prog.
21
1610-1616
2005
Aspergillus niger
Manually annotated by BRENDA team
Rutten, L.; Ribot, C.; Trejo-Aguilar, B.; Woesten, H.A.; de Vries, R.P.
A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol
BMC Microbiol.
9
166
2009
Aspergillus niger (A2QAC0), Aspergillus niger
Manually annotated by BRENDA team
Kim, B.; Sullivan, R.P.; Zhao, H.
Cloning, characterization, and engineering of fungal L-arabinitol dehydrogenases
Appl. Microbiol. Biotechnol.
87
1407-1414
2010
Aspergillus niger (A2QAC0), Penicillium chrysogenum (B6HI95), Penicillium chrysogenum Wisconsin 54-1255 (B6HI95), Trichoderma reesei (Q96V44)
Manually annotated by BRENDA team