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EC 6.3.4.15 Details

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EC number

6.3.4.15

Accepted name

biotin—[biotin carboxyl-carrier protein] ligase

Reaction

ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N⁶-biotinyl-L-lysine

Other name(s)

birA (gene name), HLCS (gene name), HCS1 (gene name), biotin-[acetyl-CoA carboxylase] synthetase, biotin-[acetyl coenzyme A carboxylase] synthetase, acetyl coenzyme A holocarboxylase synthetase, acetyl CoA holocarboxylase synthetase, biotin:apocarboxylase ligase, Biotin holoenzyme synthetase, biotin:apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ligase (AMP-forming), biotin—[acetyl-CoA-carboxylase] ligase

Systematic name

biotin:apo-[carboxyl-carrier protein] ligase (AMP-forming)

CAS registry number

37340-95-7

Comment

The enzyme biotinylates a biotin carboxyl-carrier protein that is part of an acetyl-CoA carboxylase complex, enabling its subsequent carboxylation by EC 6.3.4.14, biotin carboxylase. The carboxyl group is eventually transferred to acetyl-CoA by EC 2.1.3.15, acetyl-CoA carboxytransferase. In some organisms the carrier protein is part of EC 6.4.1.2, acetyl-CoA carboxylase.

History

created 1978, modified 2018

EC Tree

6.1 Forming carbon-oxygen bonds

6.2 Forming carbon-sulfur bonds

6.3 Forming carbon-nitrogen bonds

6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

6.3.2 Acid—amino-acid ligases (peptide synthases)

6.3.3 Cyclo-ligases

6.3.4 Other carbon-nitrogen ligases

6.3.4.1 GMP synthase

6.3.4.2 CTP synthase (glutamine hydrolysing)

6.3.4.3 formate—tetrahydrofolate ligase

6.3.4.4 adenylosuccinate synthase

6.3.4.5 argininosuccinate synthase

6.3.4.6 urea carboxylase

6.3.4.7 ribose-5-phosphate—ammonia ligase

6.3.4.8 imidazoleacetate—phosphoribosyldiphosphate ligase

6.3.4.9 biotin—[methylmalonyl-CoA-carboxytransferase] ligase

6.3.4.10 biotin—[propionyl-CoA-carboxylase (ATP-hydrolysing)] ligase

6.3.4.11 biotin—[methylcrotonoyl-CoA-carboxylase] ligase

6.3.4.12 glutamate—methylamine ligase

6.3.4.13 phosphoribosylamine—glycine ligase

6.3.4.14 biotin carboxylase

6.3.4.15 biotin—[biotin carboxyl-carrier protein] ligase

6.3.4.16 carbamoyl-phosphate synthase (ammonia)

6.3.4.17 formate—dihydrofolate ligase

6.3.4.18 5-(carboxyamino)imidazole ribonucleotide synthase

6.3.4.19 tRNA^Ile-lysidine synthase

6.3.4.20 7-cyano-7-deazaguanine synthase

6.3.4.21 nicotinate phosphoribosyltransferase

6.3.4.22 tRNA^Ile²-agmatinylcytidine synthase

6.3.4.23 formate—phosphoribosylaminoimidazolecarboxamide ligase

6.3.4.24 tyramine—L-glutamate ligase

6.3.4.25 2-amino-2′-deoxyadenylo-succinate synthase

6.3.5 Carbon-nitrogen ligases with glutamine as amido-N-donor

6.4 Forming carbon-carbon bonds

6.5 Forming phosphoric-ester bonds

6.6 Forming nitrogen—metal bonds

6.7 Forming nitrogen-nitrogen bonds

7 Translocases