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3.4.23.20: Penicillopepsin

This is an abbreviated version!
For detailed information about Penicillopepsin, go to the full flat file.

Word Map on EC 3.4.23.20

Reaction

Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1', but also cleaving Gly20-/-Glu in the B chain of insulin. Clots milk, and activates trypsinogen =

Synonyms

Acid protease A, EC 3.4.23.6, EC 3.4.23.7, EC 3.4.4.17, mold kinase, More, pencillopepsin-JT1, pencillopepsin-JT2, Penicillium citrinum acid proteinase, Penicillium cyclopium acid proteinase, Penicillium expansum acid proteinase, Penicillium janthinellum acid proteinase, Penicillium janthinellum aspartic proteinase, penicillium kinase, Penicillium roqueforti acid proteinase, penicillopepsin-JT1, penicillopepsin-JT2, penicillopepsin-JT3, PEP, PepA, Peptidase A, Proteinase, Penicillium aspartic, Proteinase, Penicillium caseicolum aspartic, Proteinase, Penicillium citrinum aspartic, Proteinase, Penicillium cyclopium acid, Proteinase, Penicillium duponti aspartic, Proteinase, Penicillium expansum aspartic, Proteinase, Penicillium janthinellum acid, Proteinase, Penicillium roqueforti aspartic

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.20 Penicillopepsin

KM Value

KM Value on EC 3.4.23.20 - Penicillopepsin

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.075
Ac-Ala-Ala-Ala-Lys-(4-nitro)-Phe-Ala-Ala amide
-
pH 5.5 (pH-optimum for kcat)
0.39 - 0.68
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
0.25
Ac-Ala-Ala-Lys-(4-nitro)Phe amide
-
pH 4.5 (pH optimum for kcat)
0.078 - 0.44
Ac-Ala-Ala-Lys-(4-nitro)Phe-Ala-Ala amide
0.32 - 0.59
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide
0.35 - 0.71
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide
0.37 - 0.8
Ac-Ala-Ala-Lys-(NO2)Phe-amide
0.41
Ac-Ala-Lys-(4-nitro)Phe amide
-
pH 4.3 (optimum for kcat)
0.41 - 0.6
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide
0.3 - 0.83
Ac-Ala-Lys-(NO2)Phe-Ala-amide
0.4 - 0.75
Ac-Ala-Lys-(NO2)Phe-amide
0.07
Ac-Gly-Lys-(4-nitro)Phe-Ala-Ala amide
-
pH 4.5
0.22
Ac-Lys 4-nitrophenyl amide
-
-
0.6
Ac-Lys-(4-nitro)Phe amide
-
pH 3.8-4.2 (optimum for kcat)
0.21 - 0.77
Ac-Lys-(NO2)Phe-Ala-Ala-amide
0.5 - 0.83
Ac-Lys-(NO2)Phe-Ala-amide
0.4 - 0.65
Ac-Lys-(NO2)Phe-amide
0.017
Ac-Val-Lys-(4-nitro)Phe-Ala amide
-
-
0.004 - 0.01
Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester
0.005
Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu
-
-
0.0076
Trypsinogen
-
-
additional information
additional information
-