Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.22.B70: SENP1 peptidase

This is an abbreviated version!
For detailed information about SENP1 peptidase, go to the full flat file.

Word Map on EC 3.4.22.B70

Reaction

The enzyme catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO-1, SUMO-2 and SUMO-3 to their mature forms and deconjugation of SUMO-1, SUMO-2 and SUMO-3 from targeted proteins. Deconjugates SUMO-1 from homeodomain-interacting protein kinase 2. Deconjugates SUMO-1 from histone deacetylase 1, which decreases its transcriptional repression activity. Cleavage of Gly97-/-His98 bond in the SUMO-1 precursor with release of the propeptide His-Ser-Thr-Val. Cleavage of Gly93-/-Val94 bond in the SUMO-2 precursor with release of the propeptide Val94-Thyr. Cleavage of the Gly92-/-Val93 in the SUMO-3 precursor with release of the propeptide Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe. =

Synonyms

C13orf22l, SENP, SENP1, sentrin-specific protease 1, sentrin/SUMO-specific protease 1, small ubiquitin-like modifier protein-specific protease 1, small ubiquitin-related modifier-specific isopeptidase, SUMO isopeptidase, SUMO protease, SUMO protein-specific protease 1, SUMO-specific isopeptidase, SUMO-specific protease, SUMO-specific protease 1, ubiquitin-specific protease-like 1, USPL1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B70 SENP1 peptidase

Cloned

Cloned on EC 3.4.22.B70 - SENP1 peptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the His-tagged catalytic domain of SENP1, SENP1c, aa419-aa643, from PC-3 cell cDNA library, cloned into pET28a(+) vector, and expressed in Escherichia coli strain BL21
-
crystallization of a complex of SENP1 C603A bound to full-length SUMO-1 and determination of the structure by X-ray crystallography. Two structures of SENP1 C603A in complex with the SUMO-1 precursor and RanGAP1-SUMO-1
expression as N-terminally His-tagged enzyme in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli BL21
expression of GFP-tagged SENP2 in HeLa cells
expression of wild-type and mutant enzymes in MEF, 293T, and 3T3-L1 cells, real-time quantitative PCR expression analysis
gene SP1, recombinant expression of GFP-tagged or untagged SENP1 and the catalytically dead enzyme mutant transiently in HeLa cells
-
overexpression of a catalytically inactive/dominant negative Cys-to-Ser SENP1 mutant
-
quantitative real-time PCR expression analysis
-
recombinant expression of wild-type Flag-tagged C13orf22l and C13orf22lcat mutant comprising amino acid residues 312-649 in HeLa cells
-
recombinant expression of YFP-tagged enzyme USPL1 in HeLa cell nucleoplasm, and of HA-tagged enzyme in Cajal bodies, co-expression with suicide inhibitor Strep-TEV-HA-SUMO-vinylmethylester, recombinant expression of truncated enzyme variant USPL1cat as His-GST-TEV-USPL1cat
SENP1, recombinant expression of wild-type and mutant enzymes
-
the catalytic core domain of SENP1 (amino acids 415-643) and mutants are cloned into the vector pEHISTEV and expressed as a N-terminally His-tagged protein. The recombinant proteins are expressed in Escherichia coli Bl21(DE3) cells