3.4.22.B68: EhCP4 proteinase

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For detailed information about EhCP4 proteinase, go to the full flat file.

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unique preference for valine and isoleucine at P2 and broader specificities at the P1 and P3 positions, while proline at P4 is relatively selective =

Synonyms

CP4, cysteine proteinase 4

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.B68 EhCP4 proteinase

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Results	in table
1	AA Sequence
1	Cloned(Commentary)
2	Expression
1	General Information
1	Inhibitors
1	Ki Value [mM]
4	KM Value [mM]
6	Localization
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
3	Organism
1	pH Optimum
1	pH Stability
1	Purification (Commentary)
1	Reaction
3	Reference
1	Renatured (Commentary)
2	Source Tissue
15	Substrates and Products (Substrate)
6	Synonyms

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

maximal active enzyme is obtained by reducing the denatured thioredoxin A-CP4 fusion protein with 25 mM DTT at 37°C for 1 h followed by rapid dilution of the protein into ice-cold refolding buffer (100 mM Tris-HCl pH 8.5, 100 mM NaCl, 20% (v/v) glycerol, 250 mM arginine-HCl, 2 mM EDTA, pH 8, 10 mM GSH, 1 mM L-GSSG disodium salt)

Entamoeba histolytica

C4LTT0

709110