3.4.22.B50: papain-like proteinase 2

This is an abbreviated version, for detailed information about papain-like proteinase 2, go to the full flat file.


responsible for the cleavages located at the N-terminus of the replicase polyprotein =


Erv-C, ervatamin-C, mouse hepatitis virus papain-like proteinase 2, papain-like accessory proteases, papain-like cysteine proteinase, papain-like protease, papain-like protease domain 2, papain-like proteinase, PL2-PRO, PL2pro, PLP-2, PLP2, PLpro, SARS-coronavirus papain-like protease, SARS-CoV papain-like protease, SARS-CoV PLpro, severe acute respiratory syndrome coronavirus papain-like protease


     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B50 papain-like proteinase 2


Cloned on EC 3.4.22.B50 - papain-like proteinase 2

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cloned into modified version of the pBacPAK8/His2 vector and expressed in insect cells Sf9
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-Gold (DE3) cells
expressed in HEK-293T cells
expression in Escherichia coli BL21 (DE3) and HeLa cells
expression in Escherichia coli XL-1 Blue and HeLa-MHV receptor cells
recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes, with a secretion tag, in Escherichia coli strain BL21(DE3)
vaccinia-T1-mediated expression of a cDNA clone encoding the MHV PLP2 domain