3.4.22.B14: papain-like proteinase 1
This is an abbreviated version!
For detailed information about papain-like proteinase 1, go to the full flat file.
Word Map on EC 3.4.22.B14
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3.4.22.B14
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polyproteins
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pp1a
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sars-cov-2
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rna-dependent
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coronaviruses
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mhv-a59
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preproinsulin
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nsp3
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3clpro
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plpros
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remdesivir
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coronaviral
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ace2
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3-chymotrypsin-like
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positive-strand
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autoproteolytic
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admet
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wistar-furth
- 3.4.22.B14
- polyproteins
- pp1a
- sars-cov-2
-
rna-dependent
- coronaviruses
-
mhv-a59
-
preproinsulin
- nsp3
- 3clpro
- plpros
- remdesivir
- coronaviral
- ace2
-
3-chymotrypsin-like
-
positive-strand
-
autoproteolytic
-
admet
-
wistar-furth
Reaction
papain-like proteinase 1 is responsible for cleavages located at the N-terminus of the replicase polyprotein =
Synonyms
C16.001, first papain-like cysteine proteinase, mouse hepatitis virus papain-like proteinase 1, PL1, PL1-PRO, PL1pro, PLP-1, PLP1, PLP1 proteinase, PLpro, replicase polyprotein 1a
ECTree
3.4.22.B14 papain-like proteinase 1Advanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.22.B14 - papain-like proteinase 1
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
?
L-Arg-L-Leu-L-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O
L-Arg-L-Leu-L-Arg-Gly-Gly + 7-amino-4-methylcoumarin
-
-
-
-
?
proISG + H2O
?
i.e. proenzyme of interferon-stimulated gene product 15
-
-
?
tetra-ubiquitin + H2O
2 di-ubiquitin
-
-
-
-
?
ubiquitinated RIG-I + H2O
ubiquitin + RIG-I
-
substrate is a key fegulator in the IFN signaling pathway
-
-
?
ubiquitinated STING + H2O
ubiquitin + STINGI
-
substrate is a key fegulator in the IFN signaling pathway
-
-
?
4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
?
-
-
-
?
4-[[4-(dimethylamino)phenyl]azo]benzoic acid-MYNKMGGGDKTVSF-(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid) amide + H2O
?
-
-
-
?
ISG15-7-amido-4-methylcoumarin + H2O
ISG15 + 7-amino-4-methylcoumarin
-
-
-
-
?
ISG15-7-amido-4-methylcoumarin + H2O
ISG15 + 7-amino-4-methylcoumarin
-
-
-
-
?
KAGG-7-amido-4-methylcoumarin + H2O
KAGG + 7-amino-4-methylcoumarin
-
-
-
?
KAGG-7-amido-4-methylcoumarin + H2O
KAGG + 7-amino-4-methylcoumarin
-
-
-
?
KKAG-7-amido-4-methylcoumarin + H2O
KKAG + 7-amino-4-methylcoumarin
-
-
-
?
KKAG-7-amido-4-methylcoumarin + H2O
KKAG + 7-amino-4-methylcoumarin
-
-
-
?
LRGG-7-amido-4-methylcoumarin + H2O
LRGG + 7-amino-4-methylcoumarin
-
-
-
?
LRGG-7-amido-4-methylcoumarin + H2O
LRGG + 7-amino-4-methylcoumarin
-
-
-
?
ORF 1a polyprotein + H2O
?
cleavage sites are: Gly247-/-Val248 and Gly821-/-Val822. PLP-1 is responsible for the cleavage of the amino-terminal protein p28
-
-
?
ORF 1a polyprotein + H2O
?
cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle
-
-
?
ORF 1a polyprotein + H2O
?
the enzyme is involved in processing of ORF 1a polyprotein into mature replicase-related polypeptides
-
-
?
ORF 1a polyprotein + H2O
?
cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle
-
-
?
ORF 1a polyprotein + H2O
?
cleavage sites are: Gly247-/-Val248 and Gly821-/-Val822. PLP-1 is responsible for the cleavage of the amino-terminal protein p28
-
-
?
ORF 1a polyprotein + H2O
?
the enzyme is involved in processing of ORF 1a polyprotein into mature replicase-related polypeptides
-
-
?
ORF 1a polyprotein + H2O
nsp1 + nsp2 + nsp3
-
SARS coronavirus replicase polyproteins pp1a and pp1ab are predicted to be processed into 16 nonstructural proteins, NSP2-3 intermediate identified
identified products
-
?
ORF 1a polyprotein + H2O
nsp1 + nsp2 + nsp3
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SARS coronavirus replicase polyproteins pp1a and pp1ab are predicted to be processed into 16 nonstructural proteins, NSP2-3 intermediate identified
identified products
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
additional information
?
-
PLpro can degrade Lys48- and Lys63-linked polyubiquitin chains to monoubiquitin but not linear polyubiquitin
-
-
?
additional information
?
-
PLpro can degrade Lys48- and Lys63-linked polyubiquitin chains to monoubiquitin but not linear polyubiquitin
-
-
?
additional information
?
-
-
enzyme is able to cleave ubiquitin mutant L73P in K6-, K11-, K48- and K63-linked di-ubiquitin chains. Cleavage rates with MERS PLpro are nearly identical against ubiquitin chains of various lengths
-
-
?
additional information
?
-
-
MERS PLpro has an absolute requirement for Gly in the P2 position, but can accommodate a wide variety of hydrophobic amino acids in both the P3 and P4 positions. The most preferred amino acid in P3 is Leu, followed by Arg, Lys and hydrophobic residues. The most preferred amino acid in P4 is also Leu, followed by mostly hydrophobic residues
-
-
?
additional information
?
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viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da
-
-
?
additional information
?
-
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viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da
-
-
?
additional information
?
-
the virus can regulate in vivo proteinase activity through self inactivation of PLP-1. The lack of p50 production in cells infected with the mouse hepatitis virus strain JHM may regulate PLP-1 activity differently from MHV-59
-
-
?
additional information
?
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the virus can regulate in vivo proteinase activity through self inactivation of PLP-1. The lack of p50 production in cells infected with the mouse hepatitis virus strain JHM may regulate PLP-1 activity differently from MHV-59
-
-
?
additional information
?
-
viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da
-
-
?
additional information
?
-
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interactions of PLP1 with CS1 and CS2 are critical for protein processing and suggest that the interactions play specific roles in regulation of the functions of nsp1, 2, and 3 in viral RNA synthesis
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-
?
additional information
?
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SARS PLpro recognizes a K48-linked tri-Ub as a minimal unit for ubiquitin chain processing
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-
?
additional information
?
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PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones
-
-
?
additional information
?
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PL1pro cleaves a peptide mimicking the cognate nsp2-nsp3 cleavage site, has deubiquitinating activity in an in vitro cleavage assay and displays a slight preference for Lys48-linked polyubiquitin chains over Lys63-linked ones
-
-
?
