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50S ribosomal protein L7/L12 + H2O
?
6-phosphogluconate dehydrogenase + H2O
?
acetyl-Lys-p-nitroaniline + H2O
acetyl-Lys + p-nitroaniline
-
-
?
acid soluble collagen + H2O
?
-
from the cartilages of brownbanded bamboo shark Chiloscyllium punctatum and blacktip shark Carcharhinus limbatus
-
-
?
ACTH + H2O
?
-
hydrolysis of the bonds: Lys11-Pro12, Lys16-Lys16, Lys16-Arg17, Lys21-Val22
-
-
?
acyl carrier protein + H2O
?
albacore tuna pepsin-solubilised collagen + H2O
?
-
-
-
-
?
amyloid beta-protein + H2O
?
B-chain of insulin + H2O
?
benzoyl-Arg ethyl ester + H2O
benzoyl-Arg + ethanol
-
-
-
-
?
benzoyl-L-Lys-4-nitroanilide + H2O
benzoyl-L-Lys + 4-nitroaniline
benzoyl-Lys methyl ester + H2O
benzoyl-Lys + methanol
benzoyl-Lys-NH2 + H2O
benzoyl-Lys + NH3
-
-
-
-
?
benzoyl-Lys-p-nitroanilide + H2O
benzoyl-Lys + p-nitroaniline
benzoyl-Lys-p-nitroaniline + H2O
benzoyl-Lys + p-nitroaniline
-
-
?
benzoyl-Orn methyl ester + H2O
benzoyl-Orn + methanol
-
-
?
Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
Boc-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
-
-
?
bovine trypsinogen + H2O
trypsin + ?
-
the enzyme activates the zymogens
-
-
?
chaperone protein DNA kinase + H2O
?
chymotrypsinogen + H2O
chymotrypsin + ?
-
-
-
-
?
Denatured hemoglobin + H2O
?
-
-
-
-
?
elongation factor G + H2O
?
elongation factor Tu + H2O
?
fibrinogen + H2O
fibrin + ?
-
-
-
-
?
fructose-bisphosphate aldolase class 2 + H2O
?
glyceraldehyde-3-phosphate dehydrogenase A + H2O
?
Ile-Asn-Leu-Lys-Ala-leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2
?
-
i.e. mastoparan. Cleavage sites: Ile-Asn-Leu-Lys-/-Ala-Leu-Ala-Ala-Leu-Ala-Lys-/-Lys-/-Ile-Leu-NH2
-
-
?
Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2 + H2O
?
-
i.e. mastoparan, cleavage sites: Lys4-Ala5, Lys11-Lys12, Lys12-Ile13
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
Lys-p-nitroanilide + H2O
Lys + p-nitroaniline
lysine vasopressin + H2O
?
N-acetyl-L-lysine + H2O
N-acetyl-L-lysine + H2O
-
-
incorporation of O18 atom into the carboxyl group
-
?
N-benzoyl-L-Lys-p-nitroanilide + H2O
N-benzoyl-L-Lys + p-nitroaniline
N-benzoyl-L-Orn methyl ester + H2O
N-benzoyl-L-Orn + methanol
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide + H2O
N-tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide + H2O
N-tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin
-
-
-
-
?
N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O
N-tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin
-
-
-
-
?
N-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-tosyl-Gly-Pro-Lys + p-nitroaniline
N-tosyl-L-Arg methyl ester + H2O
N-tosyl-L-Arg + methanol
N-tosyl-L-Lys methyl ester + H2O
N-tosyl-L-Lys + methanol
native hemoglobin + H2O
?
-
-
-
-
?
oxidized insulin B chain + H2O
?
pepsin soluble collagen + H2O
?
-
from the cartilages of brownbanded bamboo shark Chiloscyllium punctatum and blacktip shark Carcharhinus limbatus
-
-
?
phosphoglycerate kinase + H2O
?
plasminogen + H2O
plasmin + ?
-
-
-
-
?
porcine pepsin-solubilised collagen + H2O
?
-
is more resistant to hydrolysis compared with albacore tuna pepsin-solubilised collagen
-
-
?
pyroglutamyl-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn + H2O
pyroglutamyl-Ala-Lys + Ser-Gln-Gly-Gly-Ser-Asn
-
i.e. serum thymic factor
-
-
?
serum thymic factor + H2O
?
-
cleavage of the Lys-Ser bond
-
-
?
single-chain human insulin-like peptide 5 precursor + H2O
two-chain human insulin-like peptide 5
-
endoproteinase Lys-C can cleave the peptide bond at the C-terminal side of lysine residues
-
-
?
somatropin + H2O
?
-
protein digested with either trypsin or endoproteinase Lys-C prior to MALDI-MS analysis as a rapid method based on MALDI-TOF-MS peptide mass finger printing for identification of somatropin: sequence coverage obtained from individual trypsin and Lys-C is 79% and 60%, respectively, sequence coverage of both maps together is 98%
-
-
?
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin
-
-
-
?
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin
-
-
-
?
tert-butyloxycarbonyl-Lys-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin
-
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
tosyl-Gly-Pro-Lys + 4-nitroaniline
tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
tosyl-Gly-Pro-Lys + p-nitroaniline
tosyl-Lys methyl ester + H2O
tosyl-Lys + methanol
Trypsinogen + H2O
?
-
-
-
-
?
Type I collagen + H2O
?
-
from calf skin
-
-
?
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr + H2O
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys + Val-Val-Thr
-
i.e. dynorphin B
-
?
Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr + H2O
Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys + Ser-Gln-Thr-Pro-Leu-Val-Thr
-
i.e. alpha-endorphin
-
?
Val-Leu-Lys-p-nitroanilide + H2O
Val-Leu-Lys + p-nitroaniline
-
-
-
-
?
yellowfin tuna pepsin-solubilised collagen + H2O
?
-
undergoes hydrolysis to the highest extent
-
-
?
additional information
?
-
50S ribosomal protein L7/L12 + H2O
?
cleavage into 5 peptide fragments
-
-
?
50S ribosomal protein L7/L12 + H2O
?
cleavage into 5 peptide fragments
-
-
?
6-phosphogluconate dehydrogenase + H2O
?
cleavage into 7 peptide fragments
-
-
?
6-phosphogluconate dehydrogenase + H2O
?
cleavage into 6 peptide fragments
-
-
?
acyl carrier protein + H2O
?
cleavage into 3 peptide fragments
-
-
?
acyl carrier protein + H2O
?
cleavage into 3 peptide fragments
-
-
?
amyloid beta-protein + H2O
?
only the Lys-X bond is hydrolyzed
-
?
amyloid beta-protein + H2O
?
only the Lys-X bond is hydrolyzed
-
?
B-chain of insulin + H2O
?
-
only cleavage of the bond Lys29-Ala30
-
-
?
B-chain of insulin + H2O
?
-
protease I1 cleaves Lys29-Ala30 bond
-
-
?
B-chain of insulin + H2O
?
-
protease I1 cleaves Lys29-Ala30 bond
-
-
?
B-chain of insulin + H2O
?
-
only cleavage of the bond Lys29-Ala30
-
-
?
benzoyl-L-Lys-4-nitroanilide + H2O
benzoyl-L-Lys + 4-nitroaniline
-
-
-
?
benzoyl-L-Lys-4-nitroanilide + H2O
benzoyl-L-Lys + 4-nitroaniline
-
-
-
?
benzoyl-Lys methyl ester + H2O
benzoyl-Lys + methanol
-
-
-
-
?
benzoyl-Lys methyl ester + H2O
benzoyl-Lys + methanol
-
-
?
benzoyl-Lys methyl ester + H2O
benzoyl-Lys + methanol
-
-
?
benzoyl-Lys-p-nitroanilide + H2O
benzoyl-Lys + p-nitroaniline
-
-
-
-
?
benzoyl-Lys-p-nitroanilide + H2O
benzoyl-Lys + p-nitroaniline
-
-
?
casein + H2O
?
-
-
-
-
?
chaperone protein DNA kinase + H2O
?
cleavage into 10 peptide fragments
-
-
?
chaperone protein DNA kinase + H2O
?
cleavage into 9 peptide fragments
-
-
?
elongation factor G + H2O
?
cleavage into 8 peptide fragments
-
-
?
elongation factor G + H2O
?
cleavage into 5 peptide fragments
-
-
?
elongation factor Tu + H2O
?
cleavage into 7 peptide fragments
-
-
?
elongation factor Tu + H2O
?
cleavage into 5 peptide fragments
-
-
?
enolase + H2O
?
cleavage into 4 peptide fragments
-
-
?
enolase + H2O
?
cleavage into 4 peptide fragments
-
-
?
fructose-bisphosphate aldolase class 2 + H2O
?
cleavage into 5 peptide fragments
-
-
?
fructose-bisphosphate aldolase class 2 + H2O
?
cleavage into 4 peptide fragments
-
-
?
Glucagon + H2O
?
only the Lys-X bond is hydrolyzed
-
?
Glucagon + H2O
?
specific hydrolysis of lysyl peptide bond
-
-
?
Glucagon + H2O
?
specific hydrolysis of lysyl peptide bond
-
-
?
Glucagon + H2O
?
only the Lys-X bond is hydrolyzed
-
?
glyceraldehyde-3-phosphate dehydrogenase A + H2O
?
cleavage into 5 peptide fragments
-
-
?
glyceraldehyde-3-phosphate dehydrogenase A + H2O
?
cleavage into 6 peptide fragments
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
slow reaction
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
slow reaction
-
-
?
Lys-p-nitroanilide + H2O
Lys + p-nitroaniline
-
-
-
-
?
Lys-p-nitroanilide + H2O
Lys + p-nitroaniline
-
-
-
-
?
lysine vasopressin + H2O
?
-
only the Lys8-Gly9 bond is hydrolyzed
-
-
?
lysine vasopressin + H2O
?
-
only the Lys8-Gly9 bond is hydrolyzed
-
-
?
N-benzoyl-L-Lys-p-nitroanilide + H2O
N-benzoyl-L-Lys + p-nitroaniline
-
-
-
-
?
N-benzoyl-L-Lys-p-nitroanilide + H2O
N-benzoyl-L-Lys + p-nitroaniline
-
-
-
-
?
N-benzoyl-L-Lys-p-nitroanilide + H2O
N-benzoyl-L-Lys + p-nitroaniline
-
-
-
-
?
N-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-tosyl-Gly-Pro-Lys + p-nitroaniline
-
-
-
-
?
N-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-tosyl-Gly-Pro-Lys + p-nitroaniline
-
-
-
-
?
N-tosyl-L-Arg methyl ester + H2O
N-tosyl-L-Arg + methanol
-
-
-
-
?
N-tosyl-L-Arg methyl ester + H2O
N-tosyl-L-Arg + methanol
-
-
-
-
?
N-tosyl-L-Lys methyl ester + H2O
N-tosyl-L-Lys + methanol
-
-
-
-
?
N-tosyl-L-Lys methyl ester + H2O
N-tosyl-L-Lys + methanol
-
-
-
-
?
N-tosyl-L-Lys methyl ester + H2O
N-tosyl-L-Lys + methanol
-
-
-
-
?
oxidized insulin B chain + H2O
?
only the Lys-X bond is hydrolyzed
-
?
oxidized insulin B chain + H2O
?
only the Lys-X bond is hydrolyzed
-
?
phosphoglycerate kinase + H2O
?
cleavage into 4 peptide fragments
-
-
?
phosphoglycerate kinase + H2O
?
cleavage into 4 peptide fragments
-
-
?
Substance P + H2O
?
-
hydrolysis of the bond Lys3-Pro4
-
-
?
Substance P + H2O
?
-
hydrolysis of the bond Lys3-Pro4
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
-
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
-
-
?
tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
tosyl-Gly-Pro-Lys + 4-nitroaniline
-
a chromogenic substrate
-
-
?
tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
tosyl-Gly-Pro-Lys + 4-nitroaniline
-
a chromogenic substrate
-
-
?
tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
tosyl-Gly-Pro-Lys + 4-nitroaniline
-
a chromogenic substrate
-
-
?
tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
tosyl-Gly-Pro-Lys + p-nitroaniline
-
-
-
-
?
tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
tosyl-Gly-Pro-Lys + p-nitroaniline
-
-
-
-
?
tosyl-Lys methyl ester + H2O
tosyl-Lys + methanol
-
-
?
tosyl-Lys methyl ester + H2O
tosyl-Lys + methanol
-
-
-
-
?
additional information
?
-
-
the enzyme cleaves only the Lys-X bond
-
-
?
additional information
?
-
-
the enzyme cleaves only the Lys-X bond
-
-
?
additional information
?
-
-
the enzyme cleaves only the Lys-X bond
-
-
?
additional information
?
-
-
no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide
-
-
?
additional information
?
-
-
peptide maps of those collagens from both shark species digested by lysyl endopeptidase are different and are completely different from those of type I collagen from calf skin: calf skin collagen is more susceptible to hydrolysis by lysyl endopeptidase than acid soluble collagen or pepsin soluble collagen from both shark cartilages, possibly due to the higher amount of lysine residues in calf skin collagen
-
-
?
additional information
?
-
-
the peptide maps of different pepsin-solubilised collagens hydrolysed by lysyl endopeptidase are different, suggesting that there may be some differences in their primary structures, especially the alpha-helix strand, in terms of sequence as well as amino acid composition
-
-
?
additional information
?
-
-
neutron structure analysis of catalytic triad with Trp169 and His210. His57 is double protonated and forms hydrogen bonds to Ser194Ogamma and Asp113Odelta1, Odelta2. Resolution of data 2.0 A
-
-
?
additional information
?
-
the enzyme is specific for the C-terminal side of lysine residues, protein and peptide digestion efficiency, usage of quantitative mass spectrometry, analysis of miscleavage and non-cleavage, overview
-
-
?
additional information
?
-
-
neutron structure analysis of catalytic triad with Trp169 and His210. His57 is double protonated and forms hydrogen bonds to Ser194Ogamma and Asp113Odelta1, Odelta2. Resolution of data 2.0 A
-
-
?
additional information
?
-
-
no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide
-
-
?
additional information
?
-
-
the enzyme cleaves only the Lys-X bond
-
-
?
additional information
?
-
-
the enzyme cleaves only the Lys-X bond
-
-
?
additional information
?
-
-
the enzyme cleaves only the Lys-X bond
-
-
?
additional information
?
-
-
endoproteinase lysine-C (Lys-C) can specifically identify and cleave C-terminal lysine with high efficiency and specificity
-
-
?
additional information
?
-
-
endoproteinase lysine-C (Lys-C)/trypsin sequential digestion can be used in human 293T cell proteomics sample preparation, evaluation of the advantages of Lys-C/trypsin sequential digestion over trypsin digestion in solution conditions in different aspects, method optimzation, 2 M urea and trypsin in 50:1 or 100:1 ratio, overview. The peptides obtained by sequential digestion are of proper length and can be easily extracted from the gel so as to be more suitable for mass spectrometric identification
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
the enzyme is specific for the C-terminal side of lysine residues, protein and peptide digestion efficiency, usage of quantitative mass spectrometry, analysis of miscleavage and non-cleavage, overview
-
-
?
additional information
?
-
no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide
-
?
additional information
?
-
-
no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide
-
?
additional information
?
-
no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide
-
?
additional information
?
-
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin is a poor substrate
-
-
?
additional information
?
-
the enzyme has a Lys-specific serine endopeptidase activity and requires processing by cysteine protease Rgp for its activation
-
-
?
additional information
?
-
-
strict specificity: only peptide, ester, and amide bonds containing the carbonyl group of lysine are hydrolyzed
-
-
?
additional information
?
-
-
the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview
-
-
?
additional information
?
-
-
the enzyme is a corneal virulence factor of Pseudomonas aeruginosa
-
-
?
additional information
?
-
-
the enzyme is a corneal virulence factor of Pseudomonas aeruginosa
-
-
?
additional information
?
-
-
the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview
-
-
?
additional information
?
-
-
Chinese hamster ovary-expressed monoclonal antibody subjected to limited digestion using the endoproteinase Lys-C and reduced to generate Fab heavy chain, single chain Fc and light chain fragments (ca. 25 kDa each)
-
-
?
recombinant beta2-microglobulin + H2O
additional information
-
-
-
production of nine peptides: K1 (Glu4-Lys6), K2 (Ile7-lys19), K3 (Ser20-Lys41), K4 (Asn42-Lys48), K5 (Val49-Lys58), K6 (Asp59-Lys75), K7 (Asp76-Lys91), K8 (Ile92-Lys94), and K9 (Trp95-Met99). K3 and K7 are linked by a disulfide bond between Cys25 and Cys80
?