3.4.21.12: alpha-lytic endopeptidase

This is an abbreviated version!
For detailed information about alpha-lytic endopeptidase, go to the full flat file.

Reaction

preferential cleavage: Ala-/-, Val-/- in bacterial cell walls, elastin and other proteins =

Synonyms

ALP, Alpha-lytic endopeptidase, alpha-lytic protease, alpha-lytic proteinase, alphaLP, bacteriolytic protease L5, Mycobacterium sorangium alpha-lytic proteinase, Myxobacter 495 alpha-lytic proteinase, Myxobacter alpha-lytic proteinase, protein L5, proteinase, Mycobacterium sorangium alpha-lytic, proteinase, Myxobacter alpha-lytic

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.12 alpha-lytic endopeptidase

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Results	in table
1	Activating Compound
134	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
9	Crystallization (Commentary)
4	General Stability
16	Inhibitors
1	KM Value [mM]
7	Localization
2	Molecular Weight [Da]
45	Organism
48	PDB ID
2	Posttranslational Modification
21	Protein Variants
3	Purification (Commentary)
2	Reaction
1	Reaction Type
29	Reference
3	Source Tissue
1	Specific Activity [micromol/min/mg]
27	Substrates and Products (Substrate)
23	Synonyms

Substrates Products

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Substrates Products on EC 3.4.21.12 - alpha-lytic endopeptidase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

Abz-Ala-Ala-Phe-4-nitroanilide + H2O

Abz-Ala-Ala-Phe + 4-nitroaniline

Lysobacter sp. XL1

D2K8B4

-

752568

-

-

?

acetyl-Ala-Pro-Ala-4-nitroanilide + H2O

?

Lysobacter enzymogenes

-

-

666723

-

-

?

casein + H2O

?

2 entries

Elastin + H2O

?

2 entries

insulin + H2O

?

2 entries

N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O

N-succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline

2 entries

peptidoglycan + H2O

?

2 entries

Staphylococcus aureus peptidoglycan + H2O

?

Lysobacter sp. XL1

D2K8B4

the enzyme possesses a Gly-Gly endopeptidase activity with respect to staphylococcal peptidoglycan and an amidase that manifests an N-acetylmuramoyl-L-Ala amidase activity with respect to this substrate

752568

-

-

?

succinyl-Ala-Ala-Pro-Ala-4-nitroanilide

?

Lysobacter enzymogenes

-

-

650127

-

650127

?

succinyl-Ala-Ala-Pro-Ala-4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Ala + 4-nitroaniline

Lysobacter enzymogenes

-

high activity with wild-type enzyme and mutant enzyme M190A

653783

-

653783

?

succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline

Lysobacter enzymogenes

-

weak activity with wild-type enzyme, high activity with mutant enzyme M190A

653783

-

653783

?

succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline

Lysobacter enzymogenes

-

weak activity with wild-type enzyme, high activity with mutant enzyme M190A

653783

-

653783

?

succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O

succinyl-Ala-Ala-Pro-Val + 4-nitroaniline

Lysobacter enzymogenes

-

high activity with wild-type enzyme and mutant enzyme M190A

653783

-

653783

?

succinyl-Ala-Ala-Pro-X-p-nitroanilide + H2O

?

Lysobacter enzymogenes

-

X: Gly, Thr, Val, Leu, Ile, Met, Phe

36497

-

-

?

succinyl-Ala-Pro-Ala-p-nitroanilide + H2O

?

Lysobacter enzymogenes

-

-

36496, 36498

-

-

?

tert-butyloxycarbonyl-Ala-p-nitrophenyl ester + H2O

?

Lysobacter enzymogenes

-

-

36496

-

-

?

additional information

?

-

5 entries

casein + H2O

?

unidentified myxobacterium

-

-

36494

-

-

?

casein + H2O

?

unidentified myxobacterium 495

-

-

36494

-

-

?

Elastin + H2O

?

unidentified myxobacterium

-

-

36494

-

-

?

Elastin + H2O

?

unidentified myxobacterium 495

-

-

36494

-

-

?

insulin + H2O

?

unidentified myxobacterium

-

-

36494

-

-

?

insulin + H2O

?

unidentified myxobacterium 495

-

-

36494

-

-

?

N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O

N-succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline

Lysobacter enzymogenes

-

-

732508

-

-

?

N-succinyl-L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O

N-succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline

Lysobacter enzymogenes 495

-

-

732508

-

-

?

peptidoglycan + H2O

?

Achromobacter lyticus

-

-

36499

-

-

?

peptidoglycan + H2O

?

Achromobacter lyticus M497-1

-

-

36499

-

-

?

additional information

?

-

Lysobacter enzymogenes

-

oligopeptides on the carbonyl side of amino acids with short neutral aliphatic side-chains

36490

-

-

?

additional information

?

-

unidentified myxobacterium

-

carbonyl end of small residues as Val, Ser, Val

36492

-

-

?

additional information

?

-

unidentified myxobacterium

-

preferential cleavage of bonds adjacent to L-alanine

36494

-

-

?

additional information

?

-

unidentified myxobacterium 495

-

carbonyl end of small residues as Val, Ser, Val

36492

-

-

?

additional information

?

-

unidentified myxobacterium 495

-

preferential cleavage of bonds adjacent to L-alanine

36494

-

-

?