3.4.19.12: ubiquitinyl hydrolase 1
This is an abbreviated version!
For detailed information about ubiquitinyl hydrolase 1, go to the full flat file.
Word Map on EC 3.4.19.12
-
3.4.19.12
-
nerve
-
proteasome
-
neurodegenerative
-
fiber
-
innervation
-
deubiquitinase
-
polyglutamine
-
ataxia
-
parkinsonism
-
spinocerebellar
-
ubiquitin-proteasome
-
polyubiquitinated
-
glial
-
sars-cov-2
-
coronavirus
-
covid-19
-
calcitonin
-
neurofilament
-
fibrillary
-
neuropeptide
-
neuron-specific
-
traumatic
-
gene-related
-
cyld
-
parkin
-
polyq
-
synaptophysin
-
ubiquitin-like
-
polyproteins
-
usp7
-
ubiquitin-dependent
-
alpha-synuclein
-
deubiquitylating
-
npy
-
myenteric
-
3clpro
-
monoubiquitination
-
nedd8
-
cd45ro
-
isg15
-
intraepidermal
-
k48-linked
-
spermatogonial
-
mono-ubiquitinated
-
ubiquitin-binding
-
3c-like
-
mers-cov
-
medicine
-
isopeptide
-
degradation
-
synthesis
-
diagnostics
-
anti-sars-cov-2
-
molecular biology
-
analysis
-
concussion
-
pharmacology
-
drug development
- 3.4.19.12
- nerve
- proteasome
- neurodegenerative
- fiber
-
innervation
-
deubiquitinase
-
polyglutamine
- ataxia
- parkinsonism
- spinocerebellar
-
ubiquitin-proteasome
-
polyubiquitinated
- glial
- sars-cov-2
- coronavirus
- covid-19
- calcitonin
- neurofilament
-
fibrillary
-
neuropeptide
-
neuron-specific
-
traumatic
-
gene-related
- cyld
- parkin
-
polyq
-
synaptophysin
-
ubiquitin-like
- polyproteins
- usp7
-
ubiquitin-dependent
- alpha-synuclein
-
deubiquitylating
- npy
-
myenteric
- 3clpro
-
monoubiquitination
- nedd8
-
cd45ro
- isg15
-
intraepidermal
-
k48-linked
- spermatogonial
-
mono-ubiquitinated
-
ubiquitin-binding
-
3c-like
- mers-cov
- medicine
-
isopeptide
- degradation
- synthesis
- diagnostics
-
anti-sars-cov-2
- molecular biology
- analysis
- concussion
- pharmacology
- drug development
Reaction
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) =
Synonyms
A20, AD-019, AMSH, associated molecule with the SH3-domain of STAM, AT-3, ataxin-3, BAP1, BPLF1, BRCA1-associated protein-1, C-terminal hydrolases UCHL3, C-terminal ubiquitin hydrolase, Cezanne, CGI-70, CYLD, de-ubiquitinating enzyme, deubiquinating isopeptidase T, deubiquitinating enzyme, Doa4, Doa4 ubiquitin hydrolase, Doa4p ubiquitin isopeptidase, DUB, dUCH, EC 3.1.2.15, esterase, ubiquitin thiol-, hydrolase, ubiquitin carboxyl-terminal, hydrolase, ubiquitin carboxyl-terminal (Aplysia californica gene Ap-uch), Iso-T, isopeptidase, isopeptidase T, More, Neuron cytoplasmic protein 9.5, neuronal-specific protein gene product 9.5, OsUCH1, OsUCH2, OsUCH3, OsUCH4, OsUCH5, OTU-1, OTUB1, OTUB2, Otubain-1, Otubain-2, ovarian tumour 1, PA-700 associated isopeptidase, papain-like protease, PGP 9.5, PGP 9.5/UCHL1, PGP9.5, PGP9.5.1, PLP2, PLpro, Ub isopeptidase, ubiquitin C terminal hydrolase 1, ubiquitin C-terminal hydrolase, ubiquitin C-terminal hydrolase (Aplysia californica gene Ap-uch), ubiquitin C-terminal hydrolase 1, ubiquitin C-terminal hydrolase 37, ubiquitin C-terminal hydrolase 8, ubiquitin C-terminal hydrolase isoform L3, ubiquitin C-terminal hydrolase L-1, ubiquitin C-terminal hydrolase L1, ubiquitin C-terminal hydrolase L3, Ubiquitin C-terminal hydrolase UCH37, ubiquitin C-terminal hydrolase-1, ubiquitin C-terminal hydrolase-L1, ubiquitin C-terminal hydrolase-L3, ubiquitin C-terminal hydrolase-L5, ubiquitin C-terminal hydrorase-L1, ubiquitin carboxy terminal hydrolase-L1, ubiquitin carboxy terminal hydrolase-L3, ubiquitin carboxy-terminal hydrolase, ubiquitin carboxy-terminal hydrolase 1, ubiquitin carboxy-terminal hydrolase L1, ubiquitin carboxy-terminal hydrolase-L1, ubiquitin carboxy-terminal hydrolase-L3, ubiquitin carboxyhydrolase L3, ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase 1, ubiquitin carboxyl terminal hydrolase L1, ubiquitin carboxyl terminal hydrolase-L1, ubiquitin carboxyl-terminal hydrolase, ubiquitin carboxyl-terminal hydrolase 1, ubiquitin carboxyl-terminal hydrolase 44, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal hydrolase L-1, ubiquitin carboxyl-terminal hydrolase L1, ubiquitin carboxyl-terminal hydrolase l3, ubiquitin carboxyl-terminal hydrolase L5, ubiquitin carboxyl-terminal hydrolase-L1, ubiquitin carboxyl-terminal hydrolase-L5, ubiquitin carboxyterminal hydrolase L1, ubiquitin carboxyterminal hydrolase L3, ubiquitin carboxyterminal hydrolase-L1, ubiquitin hydrolase, ubiquitin hydrolase Uch-L1, ubiquitin hydrolase UCH-L3, ubiquitin isopeptidase, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, Ubiquitin thiolesterase L3, Ubiquitin thiolesterase L4, Ubiquitin thiolesterase L5, ubiquitin-C-terminal hydrolase L1, ubiquitin-carboxyl-terminal hydrolase PGP-9.5, ubiquitin-specific protease 44, UBPY, UCH, UCH L-1, UCH L1, UCH-1, UCH-8, UCH-L1, UCH-L2, UCH-L3, UCH-L4, UCH-L5, UCH37, UCH54, UCHL-1, UCHL-3, UCHL1, UCHL1/PGP 9.5, UCHL2, Uchl3, UCHL5, UCHL5N240, USP19, USP22, USP44, yeast ubiquitin hydrolase, YUH, YUH1
ECTree
3.4.19.12 ubiquitinyl hydrolase 1Advanced search results
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results (Substrates Products
Substrates Products on EC 3.4.19.12 - ubiquitinyl hydrolase 1
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(His)8-Ub-CTAPIII + H2O
(His)8-Ub + CTAPII
-
Ub i.e ubiquitin, CTAPII i.e. connective tissue-activating peptide
-
?
EBV ribonucleotide reductase
?
is deubiquitinated by BPLF1 1-246
-
-
?
HCF-1 + H2O
?
HCF-1 is the principal Bap1-interacting protein. It is K48- and K63-ubiquitinated. Endogenous HCF-1C is ubiquitinated at lysine-1807 and/or lysine-1808
-
-
?
head-to-tail polyubiquitin + H2O
ubiquitin
-
recombinant substrate from expression in Escherichia coli
-
-
?
inhibitor of kappa B-alpha + H2O
?
-
UCHL1 deubiquitinates, thereby resulting in nuclear factor-kappa B inactivation
-
-
?
N2-Lys-ubiquitin + H2O
ubiquitin + Lys
-
ubiquitin amide of the alpha amino group of lysine
-
?
N6-(N2-acetyl)Lys-ubiquitin + H2O
ubiquitin + N2-acetyl-Lys
-
ubiquitin amide of the alpha amino group of lysine
-
?
NEDD8-7-amido-4-methylcoumarin + H2O
NEDD8 + 7-amino-4-methylcoumarin
-
-
-
?
NEDD8-protein + H2O
NEDD8 + protein
enzyme shows dual specificity for ubiquitin-conjugates and NEDD8-conjugates
-
?
Nepsilon-(diubiquitin)-[Nalpha-actyl-L-Lys] + H2O
?
-
K48 linked diubiquitin
-
-
?
Smad7 + H2O
?
-
association with Smad7, which can act as an adaptor able to recruit UCH37 to the type I TGF-beta receptor and reverses Smurf-mediated ubiquitination
-
-
?
ubiquitin + H2O
monoubiquitin
-
UCH-L1 removes and recycles ubiquitin molecules from degraded proteins
-
-
?
ubiquitin C-terminal-4-methyl-7-amido-coumarin + H2O
ubiquitin + 4-methyl-7-amino-coumarin
-
UCHL3 is 200fold more active than UCHL1/PGP 9.5
-
-
?
ubiquitin fused to 60% of its N-terminus + H2O
ubiquitin + 60% of ubiquitin N-terminus
-
recombinant substrate, expressed in Escherichia coli
-
?
ubiquitin thiol ester of dithiothreitol + H2O
ubiquitin + dithiothreitol
-
-
-
-
?
ubiquitin-4-methylcoumarin 7-amide + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitin-4-methylcoumarine 7-amide + H2O
ubiquitin + 7-amino-4-methylcoumarine
-
-
-
?
ubiquitin-alpha-NH-dihydrofolate reductase + H2O
ubiquitin + dihydrofolate reductase
-
-
-
-
?
ubiquitin-alpha-NH-MHISPPEPESEEEEHYC + H2O
ubiquitin + MHISPPEPESEEEEHYC
-
-
-
?
ubiquitin-AMC
?
BPLF1 1-246 expresses deubiquitinating activity, cleaves ubiquitin chains linked by K48 and K63
-
-
?
ubiquitin-B domain of staphylococcal protein-A + H2O
ubiquitin + B domain of staphylococcal protein-A
and variants thereof, substrate is expressed as alpha-linked C-terminal fusion to ubiquitin
-
-
?
ubiquitin-beta1 domain of streptococcal protein G + H2O
ubiquitin + beta1 domain of streptococcal protein G
and variants thereof, substrate is expressed as alpha-linked C-terminal fusion to ubiquitin
-
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
ubiquitin-Fur4p-conjugate + H2O
ubiquitin + Fur4p
-
ubiquitin is bound to the Fur4p protein via its Lys63
-
?
ubiquitin-human carboxyl extension protein fusion protein + H2O
ubiquitin + human carboxyl extension protein
ubiquitin-magainin + H2O
magainin + ubiquitin
-
is efficiently cleaved at the carboxyl terminus of ubquitin, yielding recombinant magainin with high antimicrobial activity
-
-
?
ubiquitinyl-Smad2 + H2O
ubiquitin + Smad2
the enzyme de-ubiquitinates both Smad2 and Smad3 and up-regulates their stability
-
-
?
ubiquitinyl-Smad3 + H2O
ubiquitin + Smad3
the enzyme de-ubiquitinates both Smad2 and Smad3 and up-regulates their stability
-
-
?
ubiquitinyldihydrofolate reductase + H2O
ubiquitin + dihydrofolate reductase
-
UCH-1
-
?
Ac-ALRGG-7-amido-4-trifluoromethylcoumarin
?
-
-
-
-
?
Ac-LRGG-7-amido-4-trifluoromethylcoumarin
?
-
-
-
-
?
Ac-RLRGG-7-amido-4-trifluoromethylcoumarin
?
-
-
-
-
?
branched di-ubiquitin + H2O
ubiquitin
-
recombinant substrate from expression in Escherichia coli
-
-
?
branched di-ubiquitin + H2O
ubiquitin
-
recombinant substrate expressed in Escherichia coli
-
-
?
branched polyubiquitin + H2O
ubiquitin
-
substrate is ligated to proteins via epsilon-NH-isopeptide bonds
-
?
branched polyubiquitin + H2O
ubiquitin
isopeptide-linked ubiquitin chains
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
best substrate
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
di-ubiquitin + H2O
ubiquitin
-
active with wild-type and mutant di-ubiquitin, the latter contains a distal mutant ubiquitin L8A/I44A
-
-
?
di-ubiquitin + H2O
ubiquitin
-
recombinant linear substrate, expressed in Escherichia coli
-
-
?
linear ubiquitin-beta-galactosidase + H2O
ubiquitin + protein
-
recombinant fusion protein co-expressed with the enzyme in Escherichia coli
-
?
linear ubiquitin-beta-galactosidase + H2O
ubiquitin + protein
-
recombinant fusion protein co-expressed with the enzyme in Escherichia coli
-
?
monoubiquitin + H2O
?
-
the octapeptide amide DELRFNAI-NH2 from UCH-L3 binds to monoubiquitin
-
-
?
N6-Lys-ubiquitin + H2O
ubiquitin + Lys
-
ubiquitin amide of the epsilon amino group of lysine
-
?
N6-Lys-ubiquitin + H2O
ubiquitin + Lys
-
ubiquitin amide of the epsilon amino group of lysine
-
-
?
polyubiquitin + H2O
ubiquitin
-
hydrolysis is restricted to the distal end of the substrate, i.e. ubiquitin farthest from the site of protein attachment in poly-ubiquitin conjugates
-
-
?
polyubiquitin + H2O
ubiquitin
-
active with Lys48-, Lys6-, and Lys11-linked poly-ubiquitins
-
-
?
polyubiquitin + H2O
ubiquitin
-
no activity with poly-ubiquitins that contain a distal mutant ubiquitin L8A/I44A
-
-
?
polyubiquitin + H2O
ubiquitin
-
substrate of UCHL3, no activity with UCHL1/PGP 9.5
-
-
?
polyubiquitin + H2O
ubiquitin
-
disassembly of polyubiquitin, terminal step of the proteasome pathway, scheme
-
?
polyubiquitin + H2O
ubiquitin
-
inhibition by prostaglandins of the J series leads to accumulation of polyubiquitins of MW around 250 kDa
-
?
polyubiquitin + H2O
ubiquitin
-
Cezanne deubiquitinates Lys48- or Lys63-polyubiquitinated cellular proteins
-
-
?
polyubiquitin + H2O
ubiquitin
-
substrate of UCHL3, no activity with UCHL1/PGP 9.5
-
-
?
polyubiquitin + H2O
ubiquitin
UCHL5 deubiquitylates the polyubiquitin chain into ubiquitin
-
-
?
polyubiquitin + H2O
ubiquitin
UCHL5 deubiquitylates the polyubiquitin chain into ubiquitin
-
-
?
type I TGF-beta receptor + H2O
?
UCH37 deubiquitinates and stabilizes type I TGF-beta receptor. Overexpression of UCH37 upregulates TGF-beta-dependent transcription
-
-
?
type I TGF-beta receptor + H2O
?
-
UCH37 dramatically up-regulates TGF-beta-dependent gene expression by deubiquitinating and stabilizing the type I TGF-beta receptor
-
-
?
ubiquitin + H2O
?
-
cleavage of the G76-D77 peptide bond of (L8A, I44A, V70A)-D77 ubiquitin
-
-
?
ubiquitin metallothionein + H2O
?
-
recombinant substrate, expressed in Escherichia coli
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
ir
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
recombinant wild-type isozyme L1 and mutants I93M and S18Y
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
fluorogenic synthetic substrate
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
UCH-L1 and UCH-L3
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
ubiquitin makes modest contact with UCH-L1. The interaction is mainly electrostatic, contributed by conserved acidic residues E7, E11, D30, E35, and E37
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
recombinant wild-type isozyme L1
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
-
?
ubiquitin-alphaNH-MHISPPEPESEEEEHYC + H2O
ubiquitin + MHISPPEPESEEEEHYC
-
-
-
?
ubiquitin-alphaNH-MHISPPEPESEEEEHYC + H2O
ubiquitin + MHISPPEPESEEEEHYC
-
-
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
-
UCH-1
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
-
carboxyl extension protein of 80 amino acids
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
-
carboxyl extension protein of 80 amino acids
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
-
carboxyl extension protein of 52 amino acids
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
-
carboxyl extension protein of 38 amino acids
-
-
?
ubiquitin-CEP52 + H2O
ubiquitin + CEP52
-
substrate of UCHL1/PGP 9.5, no activity with UCHL3
-
-
?
ubiquitin-CEP52 + H2O
ubiquitin + CEP52
-
substrate of UCHL1/PGP 9.5, no activity with UCHL3
-
-
?
ubiquitin-CEP80 + H2O
ubiquitin + CEP80
-
substrate of UCHL3 and UCHL1/PGP 9.5
-
-
?
ubiquitin-CEP80 + H2O
ubiquitin + CEP80
-
substrate of UCHL3 and UCHL1/PGP 9.5
-
-
?
ubiquitin-human carboxyl extension protein fusion protein + H2O
ubiquitin + human carboxyl extension protein
-
i.e. HUbCEP-52
-
?
ubiquitin-human carboxyl extension protein fusion protein + H2O
ubiquitin + human carboxyl extension protein
-
recombinant substrate, expressed in Escherichia coli
-
?
ubiquitin-peptide + H2O
?
-
UCH enzymes may function to regenerate active ubiquitin from adducts with small nuceleophiles
-
-
?
ubiquitin-protein + H2O
ubiquitin + protein
-
enzyme is crucial for polarization of the actin cytoskeleton, to segregate germline determinants, to assemble an intact cleavage furrow, for cytokinesis and to maintain osmotic balance and execute actin-dependent processes in the early embryo
-
?
ubiquitin-protein + H2O
ubiquitin + protein
enzyme shows dual specificity for ubiquitin-conjugates and NEDD8-conjugates
-
?
ubiquitin-protein + H2O
ubiquitin + protein
important role in regulation of cell growth
-
?
ubiquitin-protein + H2O
ubiquitin + protein
-
involved in endocytotic pathway of plasma membrane proteins
-
?
ubiquitin-protein + H2O
ubiquitin + protein
-
Doap4 enzyme form plays a general role in deubiquination of membrane-bound proteins
-
?
ubiquitin-W + H2O
?
-
competent substrate for both UCH-L1 and UCH-L3
-
-
?
ubiquitinyl-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
mechanistic studies
-
-
?
ubiquitinyl-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
-
-
-
?
ubiquitinyl-peptide + H2O
ubiquitin + peptide
-
catalyses the hydrolysis of an ester or amide bond involving the C-terminal Gly76 of ubiquitin
-
-
?
ubiquitinyl-peptide + H2O
ubiquitin + peptide
-
catalyses the hydrolysis of an ester or amide bond involving the C-terminal Gly76 of ubiquitin
-
-
?
ubiquitinyl-peptide + H2O
ubiquitin + peptide
-
catalyses the hydrolysis of an ester or amide bond involving the C-terminal Gly76 of ubiquitin
-
-
?
ubiquitinyl-peptide + H2O
ubiquitin + peptide
-
UCH-L3 cleaves peptide extensions of up to 20 residues with high efficiency and low sequence preference
-
-
?
ubiquitinyl-peptide + H2O
ubiquitin + peptide
-
catalyses the hydrolysis of an ester or amide bond involving the C-terminal Gly76 of ubiquitin
-
-
?
additional information
?
-
-
enzyme contains ubiquitin-C-terminal hydrolase activity towards biosynthetic precursors of ubiquitin as well as isopeptidase activity towards ubiquitin histone conjugates as human ubiquitin carboxyl extension protein and ubiquitin C-terminal amide
-
-
?
additional information
?
-
-
purified PGP 9.5 from bovine brain exhibits a specific activity in cleaving ubiquitin-ethylester which is 40% of that shown by bovine thymus UCHL3. UCHL1/PGP 9.5 and UCHL3 hydrolysis rates of various synthetic ubiquitin conjugates compare to rates of hydrolysis of the generic substrate ubiquitin-ethylester. Both enzymes cleave ubiquitin from N-epsilon linked lysine or N-alpha-linked lysine equally well. Neither can cleave the Ub-K48 Ub bond, but both can cleave lysine from the C-terminal of this conjugate. UCHL3 can readily cleave a wide variety of peptides from the C-terminus of ubiquitin if the peptides are less than 20 residues, UCHL1/PGP 9.5 is at least a hundred times less active in this
-
-
?
additional information
?
-
-
uch-L1 may have an important role in the development of neuronal cells in early embryos as well as in the degeneration and disease of neuronal cells in late adult brain. The gene for ubiquitin C-terminal hydrolase L1 is associated with inherited forms of Parkinson's disease
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additional information
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uch-L1 may have an important role in the development of neuronal cells in early embryos as well as in the degeneration and disease of neuronal cells in late adult brain. The gene for ubiquitin C-terminal hydrolase L1 is associated with inherited forms of Parkinson's disease
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additional information
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UCH-1 releases ubiquitins that are conjugated to proteins or adjacend ubiquitin molecules by epsilonNH-isopeptide linkages as well as alphaNH-peptide bonds
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additional information
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no activity with poly-His-tagged di-ubiquitin
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increased activity of neuronal isozyme L1 reduces the risk for Parkinson's disease
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increased activity of neuronal isozyme L1 reduces the risk for Parkinson's disease
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additional information
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AMSH is a deubiquinating enzyme with functions at the endosome, it opposes the ubiquitin-dependent sorting of receptors to lysosomes, the enzyme influences the degradation of the EGF receptor by association to endosomes, model of the enzyme's physiological function
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additional information
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gene uch-L1 is involved in development of Parkinson's disease
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additional information
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gene uch-L1 is involved in development of Parkinson's disease
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additional information
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the enzyme is involved in generation of free monomeric ubiquitin, enzyme deficiency leads is involved in accumulation of ubiquitin and alpha-synuclein leading to aggregation in proteins conforming Lewy bodies and Lewy neurites leading to Parkinson's disease and dementia with Lewy bodies, the enzyme is down-regulated in Alzheimer's disease and in Parkinson's disease, comparison of enzyme expression to general protein synthesis and proteasome activity in brain
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additional information
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the full length UCH-L1 is a de-ubiquinating enzyme in brain, and a major target of oxidative damage in Alzheimer's diesease and Parkinson's disease brains, modified by cysteine and methionine oxidation and carbonyl formation
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additional information
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the full length UCH-L1 is a de-ubiquinating enzyme in brain, and a major target of oxidative damage in Alzheimer's diesease and Parkinson's disease brains, modified by cysteine and methionine oxidation and carbonyl formation
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additional information
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ubiquitin carboxy-terminal hydrolase L1 activates the P2X receptor leading to potentiation of ATP-induced currents in neurons
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additional information
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UCH-L1 is involved in Parkinson's disease
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additional information
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UCH-L1 regulates the morphology of neural progenitor cells and modulates their differentiation, it mediates and enhances neurogenesis in the embryonic brain
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additional information
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AMSH interacts with the signal transducing adaptor molecule, STAM, via the SH3-domains
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additional information
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oxidative modifications and down-regulation of ubiquitin carboxyl-terminal hydrolase L1 associated with idiopathic Parkinsons and Alzheimers diseases
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additional information
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oxidative modifications and down-regulation of ubiquitin carboxyl-terminal hydrolase L1 associated with idiopathic Parkinsons and Alzheimers diseases
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additional information
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UCHL1 is an essential gene in cell survival under normal growth conditions and against oxidative stress
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additional information
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UCHL1 is an essential gene in cell survival under normal growth conditions and against oxidative stress
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additional information
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UCHL1 is an essential gene to maintain cell homeostasis under normal growth and oxidative stress conditions
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additional information
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UCHL1 is an essential gene to maintain cell homeostasis under normal growth and oxidative stress conditions
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additional information
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UCHL1 is associated with Parkinsons disease
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additional information
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neither Uch37 alone nor the Uch37-Adrm1 or Uch37-Adrm1-S1 complexes can hydrolyse di-ubiquitin efficiently, rather incorporation into the 19S complex is required to enable processing of polyubiquitin chains
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additional information
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UCH-L1 may directly destroy or neutralize oligomeric beta-amyloid peptide and may promote degradation of beta- and gamma secretase, or amyloid precursor protein
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additional information
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wild-type UCH-L1 interacts with multiple proteins over 30 kDa. It shows no interaction with beta-actin
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Bap1 helps to control cell proliferation by regulating HCF-1 protein levels and by associating with genes involved in the G1-S transition
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epigenetic inactivation of UCHL1 is common in primary hepatocellular carcinomas and other digestive tumors. UCHL1 appears to be a functional tumor suppressor involved in the tumorigenesis of hepatocellular carcinomas and other digestive cancers. Ectopic expression of UCHL1 induces apoptosis through intrinsic caspase-dependent pathway. UCHL1 directly interacts with p53 and inhibits p53 protein degradation
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additional information
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mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L1 in vivo
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additional information
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mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L1 in vivo
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additional information
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mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L3 in vivo
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additional information
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mono-ubiquitin and ubiquitin dimers may regulate the enzymatic function of UCH-L3 in vivo
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additional information
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role for UCH-L1 and ubiquitin modification in podocyte differentiation and injury
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additional information
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role for UCH-L1 and ubiquitin modification in podocyte differentiation and injury
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additional information
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silencing of UCH-L1 in MCF7/Adr cells does not suppress cell growth. Overexpression of UCH-L1 in MCF7 cells induces apoptosis. Apoptosis triggered by UCH-L1 is, at least in part, probably through phosphoinositide 3-kinase/Akt signal pathway
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additional information
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silencing of UCH-L1 in MCF7/Adr cells does not suppress cell growth. Overexpression of UCH-L1 in MCF7 cells induces apoptosis. Apoptosis triggered by UCH-L1 is, at least in part, probably through phosphoinositide 3-kinase/Akt signal pathway
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additional information
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UCH-L1 is a key molecule to regulate tumor-cell invasion by upstream activation of Akt. Expression of UCH-L1 in tumor cells enhances their invasive potential in vitro and in vivo. UCH-L1 changes cell morphology by regulating cell adhesion through Akt-mediated pathway
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additional information
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UCH-L1 is a player in the signaling pathways that promote the proliferation and invasive capacity of malignant B-cells
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additional information
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UCH-L1 is a player in the signaling pathways that promote the proliferation and invasive capacity of malignant B-cells
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additional information
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Uch37 activities can be modulated both positively and negatively via dynamic interactions with partner proteins, whereby the proteasome and the hINO80 chromatin-remodeling complex may cooperate to regulate transcription or DNA repair
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additional information
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Uch37 activities can be modulated both positively and negatively via dynamic interactions with partner proteins, whereby the proteasome and the hINO80 chromatin-remodeling complex may cooperate to regulate transcription or DNA repair
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additional information
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Cezanne-specific siRNA suppresses Cezanne mRNA and protein by at least 70% in culture cells. It leads to elevated NF-kappaB luciferase reporter gene activity and enhanced expression of IL-8 transcripts in TNFalpha-treated cells. Endogenous Cezanne can attenuate NF-kappaB activation and the induction of pro-inflammatory transcripts in response to TNF receptor signaling. Deubiquitinating activity of Cezanne is essential for NF-kappB suppression. Cezanne can be recruited to activated TNF receptors where it suppresses the build-up of polyubiquitinated RIP1 signal adapter proteins
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additional information
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Iso-T shows high preference for the natural Leu-Arg-Gly motif in the P4P2 positions. Only reactivity with norleucine at P4 and lysine at P3 mar the exquisite specificity
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additional information
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Iso-T shows high preference for the natural Leu-Arg-Gly motif in the P4P2 positions. Only reactivity with norleucine at P4 and lysine at P3 mar the exquisite specificity
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additional information
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Iso-T shows high preference for the natural Leu-Arg-Gly motif in the P4P2 positions. Only reactivity with norleucine at P4 and lysine at P3 mar the exquisite specificity
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additional information
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Iso-T shows high preference for the natural Leu-Arg-Gly motif in the P4P2 positions. Only reactivity with norleucine at P4 and lysine at P3 mar the exquisite specificity
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additional information
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OTU-1 shows similarly to UCH-L3 high specificity in the P2 and P3 positions and less specificity in the P4 position. The P4 position prefers norleucine over leucine, but other bulky amino acids are also accepted, such as tyrosine, tryptophan, phenylalanine or lysine
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additional information
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OTU-1 shows similarly to UCH-L3 high specificity in the P2 and P3 positions and less specificity in the P4 position. The P4 position prefers norleucine over leucine, but other bulky amino acids are also accepted, such as tyrosine, tryptophan, phenylalanine or lysine
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additional information
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OTU-1 shows similarly to UCH-L3 high specificity in the P2 and P3 positions and less specificity in the P4 position. The P4 position prefers norleucine over leucine, but other bulky amino acids are also accepted, such as tyrosine, tryptophan, phenylalanine or lysine
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additional information
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OTU-1 shows similarly to UCH-L3 high specificity in the P2 and P3 positions and less specificity in the P4 position. The P4 position prefers norleucine over leucine, but other bulky amino acids are also accepted, such as tyrosine, tryptophan, phenylalanine or lysine
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additional information
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UCH-L3 does not show hydrolase activity toward the K48-linked, K63-linked or linear ubiquitin dimer
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additional information
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UCH-L3 does not show hydrolase activity toward the K48-linked, K63-linked or linear ubiquitin dimer
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additional information
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UCH-L3 shows a preference for leucine at P4, but also a broad tolerance of several other hydrophobic residues at this position. It also shows a surprising selectivity for alanine and valine at P2, in addition to the canonical glycine and high preference at the P3 position and relatively low specificity in the P4 position
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additional information
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UCH-L3 shows a preference for leucine at P4, but also a broad tolerance of several other hydrophobic residues at this position. It also shows a surprising selectivity for alanine and valine at P2, in addition to the canonical glycine and high preference at the P3 position and relatively low specificity in the P4 position
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additional information
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UCH-L3 shows a preference for leucine at P4, but also a broad tolerance of several other hydrophobic residues at this position. It also shows a surprising selectivity for alanine and valine at P2, in addition to the canonical glycine and high preference at the P3 position and relatively low specificity in the P4 position
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additional information
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UCH-L3 shows a preference for leucine at P4, but also a broad tolerance of several other hydrophobic residues at this position. It also shows a surprising selectivity for alanine and valine at P2, in addition to the canonical glycine and high preference at the P3 position and relatively low specificity in the P4 position
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additional information
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interaction of the ubiquitin carboxyl terminal esterase L1 with alpha2-adrenergic receptors inhibits agonist-mediated p44/42 MAP kinase activation. Uch-L1 binds specifically to the third intracellular loop of alpha2A adrenergic receptor, but also to the respective loops of adrenergic receptors alpha2B, alpha2C, and beta2, overview
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additional information
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polyubiquitin processing, overview. UCHL1/PGP 9.5 also shows ligase activity when in a dimeric form, overview
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additional information
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the proteasomal pathway is partially inhibited by UCH-L1M
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additional information
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UCH L1 forms endogenous complexes with beta-catenin and stabilizes it. The expression and deubiquitinating activity of UCHL1 are required for its own basic promoter activity, therefore UCH L1 up-regulates its expression by activation of the oncogenic beta-catenin/TCF signaling in transformed cells
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additional information
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UCH-L1 can hydrolyze isopeptide bonds between the C-terminal glycine of Ub and the alpha- or gamma-amino group of lysine on the target proteins. UCH-L3 recognizes and hydrolyzes isopeptide bonds at the C-terminal glycine of either ubiquitin or Nedd8, a ubiquitin-like protein. It shows no ligase activity. UCH-L3 can interact with Lys48-linked ubiquitin dimers to protect it from degradation and meanwhile to inhibit its hydrolase activity, D33 and C95 are essential for the interaction with di-ubiquitin. Neither UCH37 alone nor the UCH37-Adrm1 or UCH37-Adrm1-hRpn2 complexes can hydrolyse Lys48-linked di-ubiquitin efficiently, rather, their incorporation into the 19S complex is required to enable UCH37 to process large ubiquitin protein conjugates such as di-ubiquitin
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additional information
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UCH-L1 is a deubiquitinating enzyme that catalyses the hydrolysis of polyubiquitin precursors and small ubiquitin adducts
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additional information
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UCH-L1 is a deubiquitinating enzyme that catalyses the hydrolysis of polyubiquitin precursors and small ubiquitin adducts
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additional information
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UCH-L1 is a deubiquitinating enzyme. UCH-L1 increases the synthesis of cystic fibrosis transmembrane conductance regulator, CFTR, but has little effect on the rate of post-translational degradation
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additional information
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Phe214 is essential for distal-site substrate binding, and the loss of catalytic activity of the mutant may reflect its reduced affinity toward the substrate. The crystal structure of apo UCHL1 shows that the active-site residues are not aligned in a canonical form, with the nucleophilic cysteine being 7.7 A from the general base histidine, an arrangement consistent with an inactive form of the enzyme
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additional information
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the apparent catalytic triad of amino acids in UCHL1 is Cys90, His161 and Asp176 in the active site. The region forming a disordered crossover loop limiting access of substrates to the active site is located at positions 140-160, overview
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additional information
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UCH-L3 hydrolysis rates for the different engineered substrates are very closely correlated to the thermal stabilities of each attached test protein substrate. The thermal stabilities of the engineered substrates are not altered by fusion to ubiquitin. No substrate: alpha-linked, linear di-ubiquitin
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additional information
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UCH-L3 hydrolysis rates for the different engineered substrates are very closely correlated to the thermal stabilities of each attached test protein substrate. The thermal stabilities of the engineered substrates are not altered by fusion to ubiquitin. No substrate: alpha-linked, linear di-ubiquitin
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additional information
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shRNA decreases mRNA levels and reduces viral genome production. EBV deubiquitinating enzyme BPLF1 interacts with, deubiquitinates, and influences the activity of the Epstein-Barr virus ribonucleotide reductase
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additional information
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shRNA decreases mRNA levels and reduces viral genome production. EBV deubiquitinating enzyme BPLF1 interacts with, deubiquitinates, and influences the activity of the Epstein-Barr virus ribonucleotide reductase
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additional information
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the enzyme is necessary for placental and fetal development
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additional information
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the enzyme is necessary for placental and fetal development
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additional information
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Uch-L1, via the ubiquitin-proteasome system, may play an important role not only in gametogenesis, but also in the gonadal transformation process in the rice field eel
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additional information
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Uch-L1 binds to and interacts with monoubiqutin
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additional information
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isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism
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additional information
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isozyme L1 associates and colocalizes with monoubiquitin and elongates ubiquitin half-life, null mutants show a reduced monoubiquitin level in neurons, while overexpression causes an increase in monoubiquitin level, mechanism
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additional information
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the closely related isozymes UCH-L1 and UCH-L3 function as reciprocal modulators of germ cell apoptosis in cryptorchid testis
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additional information
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the closely related isozymes UCH-L1 and UCH-L3 function as reciprocal modulators of germ cell apoptosis in cryptorchid testis
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additional information
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the enzyme is essential for the early apoptotic wave of germinal cells and for sperm quality control during spermatogenesis
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additional information
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the enzyme is essential for the early apoptotic wave of germinal cells and for sperm quality control during spermatogenesis
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additional information
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the enzyme is involved in spermatogenesis
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additional information
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the enzyme is involved in spermatogenesis
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additional information
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the enzyme plays a role in neural cell apoptosis induced by ischemic retinal injury, e.g. in mice with gracile axonal dystrophy, overview
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additional information
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UCH-L1 functions in sperm quality control during epididymal maturation
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additional information
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UCH-L1 functions in sperm quality control during epididymal maturation
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additional information
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UCH-L1 regulates the morphology of neural progenitor cells and modulates their differentiation, it mediates and enhances neurogenesis in the embryonic brain
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additional information
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UCH-L1 regulates the morphology of neural progenitor cells and modulates their differentiation, it mediates and enhances neurogenesis in the embryonic brain
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additional information
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Uchl3 is involved in working memory, and plays a role in learning, memory, and synaptic plasticity, uchl3-deficient mice are impaired in acquisition of hippocampus-dependent tasks requiring learning and remembering, but show wild-type long-term memory for context and cued fear conditioning, uchl3-/- mice exhibit wild-type synaptic transmission and plasticity in hippocampal area CA1, overview
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additional information
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Uchl3 is involved in working memory, and plays a role in learning, memory, and synaptic plasticity, uchl3-deficient mice are impaired in acquisition of hippocampus-dependent tasks requiring learning and remembering, but show wild-type long-term memory for context and cued fear conditioning, uchl3-/- mice exhibit wild-type synaptic transmission and plasticity in hippocampal area CA1, overview
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additional information
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the enzyme shows de-ubiquinating activity
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additional information
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affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons
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additional information
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affects ubiquitin degradation and alters its metabolism. UCH-L1-mediated increases in ubiquitin levels are a function of UCH L1 affinity for ubiquitin rather than hydrolase activity. The enzyme insures ubiquitin stability within neurons
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additional information
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gracile axonal dystrophy is a syndrome that emanates from dysfunctional ubiquitin carboxyl-terminal hydrolase L-1
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additional information
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gracile axonal dystrophy is a syndrome that emanates from dysfunctional ubiquitin carboxyl-terminal hydrolase L-1
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additional information
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mice overexpressing EF1alpha promoter-driven UCH-L1 in the testis are sterile due to a block during spermatogenesis at an early stage of meiosis. Overexpression of UCH-L1 affects spermatogenesis during meiosis and, in particular, induces apoptosis in primary spermatocytes. UCH-L-1 plays a specific role in the process of mitotic proliferation and differentiation of spermatogonial stem cells during spermatogenesis
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additional information
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mice overexpressing EF1alpha promoter-driven UCH-L1 in the testis are sterile due to a block during spermatogenesis at an early stage of meiosis. Overexpression of UCH-L1 affects spermatogenesis during meiosis and, in particular, induces apoptosis in primary spermatocytes. UCH-L-1 plays a specific role in the process of mitotic proliferation and differentiation of spermatogonial stem cells during spermatogenesis
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additional information
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the enzyme may play a significant role in implantation and placental development, and differentiation of embryonic ectoderm
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additional information
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the enzyme may play a significant role in implantation and placental development, and differentiation of embryonic ectoderm
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additional information
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ubiquitin C-terminal hydrolase L3 (Uchl3) is involved in working memory
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additional information
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ubiquitin C-terminal hydrolase L3 (Uchl3) is involved in working memory
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additional information
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ubiquitin hydrolase Uch-L1 rescues beta-amyloid-induced decreases in synaptic function and contextual memory
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additional information
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ubiquitin hydrolase Uch-L1 rescues beta-amyloid-induced decreases in synaptic function and contextual memory
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additional information
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UCH-L1 may play an important role in neurodegeneration of amyloid lateral sclerosis
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additional information
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UCH-L1 may play an important role in neurodegeneration of amyloid lateral sclerosis
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additional information
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UCHL1 is required for normal spermatogenesis and sperm quality control. UCHL1-dependent apoptosis is important in spermatogonial cell and sperm maturation
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additional information
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UCHL1 is required for normal spermatogenesis and sperm quality control. UCHL1-dependent apoptosis is important in spermatogonial cell and sperm maturation
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additional information
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AT-3 carrying six consecutive glutamines undergoes fragmentation upon incubation at room temperature. Cys14 and His119 are involved in the autolytic activity
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additional information
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CYLD negatively regulates RANK signaling by inhibiting TRAF6 ubiquitination and activation of downstream signaling events. CYLD interacts physically with the signaling adaptor p62 and thereby is recruited to TRAF6. CYLD is a crucial negative regulator of osteoclastogenesis and may be involved in the p62/TRAF6 signaling axis
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additional information
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is overexpressed in alpha-tocopherol deficient mice
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additional information
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mono-ubiquitin and ubiquitin dimers may regulate the enzymatic functions of UCH-L3, in vivo
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additional information
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mono-ubiquitin and ubiquitin dimers may regulate the enzymatic functions of UCH-L3, in vivo
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additional information
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UCH-L1 expression levels show a time-dependent upregulation in mice ischemia-reperfusion injury condition. Germ cell apoptosis triggers downregulation of UCH-L1 at both mRNA and protein levels, thus, ubiquitination level is impaired. Eevidences of UCH-L1/ubiquitination signaling to the testis ischemia-reperfusion injury in vivo
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additional information
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UCH-L1 expression levels show a time-dependent upregulation in mice ischemia-reperfusion injury condition. Germ cell apoptosis triggers downregulation of UCH-L1 at both mRNA and protein levels, thus, ubiquitination level is impaired. Eevidences of UCH-L1/ubiquitination signaling to the testis ischemia-reperfusion injury in vivo
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additional information
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UCH-L3 does not function to hydrolyze free ubiquitin dimers
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additional information
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UCH-L3 does not function to hydrolyze free ubiquitin dimers
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additional information
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UCH-L1 is a deubiquitinating enzyme, it binds to and stabilize mono-ubiquitin in neurons
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additional information
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UCH-L1 is a deubiquitinating enzyme, it binds to and stabilizes mono-ubiquitin in neurons
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additional information
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UCH-L3 is capable of cleaving Ub from the Ub chains in vitro, and UCH-L3 directly hydrolyses polyubiquitinated proteins. Wild-type, but not its hydrolase activity or ubiquitin binding activity deficient, UCH-L3 shows the ability to cleave ubiquitin from polyubiquitinated lysozyme in vitro, which is no substrate of UCH-L1
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additional information
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deubiquitinating as well as deNeddylating activity. Active residues are Cys145, His220 and Asp235
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additional information
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the deubiquitinase readily processes a variety of di-Ub linkages
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additional information
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the deubiquitinase readily processes a variety of di-Ub linkages
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additional information
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de novo synthesis of isozyme UCH-L1 in rostral ventrolateral medulla is crucial to survival during mevinphos intoxication, the enzyme is involved in synthesis of ubiquitin which is required for protein targeting in degradation in the ubiquitin-proteasome pathway in neurons, UCH-L1 plays a neuroprotective role
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additional information
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de novo synthesis of isozyme UCH-L1 in rostral ventrolateral medulla is crucial to survival during mevinphos intoxication, the enzyme is involved in synthesis of ubiquitin which is required for protein targeting in degradation in the ubiquitin-proteasome pathway in neurons, UCH-L1 plays a neuroprotective role
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additional information
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overexpression of UCH L1 potentiates ATP-induced currents due to the activation of P2X receptors that are widely distributed in the brain and involved in various biological activities including neurosecretion. UCH-L1 may play an important role in synaptic activity
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additional information
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UCHL1 can act as a ubiquitin hydrolase, and generate free ubiquitin species from precursor ubiquitin polypeptides. Synaptic activation of UCH-L1 is correlated with an increase in the levels of free monomeric ubiquitin
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additional information
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core histones and H1 are not cleaved
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additional information
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Bro1-Doa4 interaction involves the C-terminal proline-rich domain of Bro1 and a conserved ELC box motif in Doa4
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additional information
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deubiquitination by Doa4 of cargo proteins and/or some components of the multivesicular body sorting machinery is essential to correct sorting of cargoes into the multivesicular body pathway
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additional information
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H6YUH1 is unable to hydrolyze the linkage of ubiquitin with human growth hormone
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additional information
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PLpro reveals very high specificity in the P4 position, where practically only leucine can be tolerated, and even higher specificity in the P2 position, with only glycine being accepted. However, the P3 position can accommodate a number of different amino acids, with some preference for the hydrophobes leucine and tyrosine, and also for the basic side chains of lysine and arginine, amino acids that possess a substantial hydrophobic character
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additional information
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UCHL3 interacts with peripheral acrosomal plasma membrane proteins
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