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3.4.19.12: ubiquitinyl hydrolase 1

This is an abbreviated version!
For detailed information about ubiquitinyl hydrolase 1, go to the full flat file.

Word Map on EC 3.4.19.12

Reaction

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) =

Synonyms

A20, AD-019, AMSH, associated molecule with the SH3-domain of STAM, AT-3, ataxin-3, BAP1, BPLF1, BRCA1-associated protein-1, C-terminal hydrolases UCHL3, C-terminal ubiquitin hydrolase, Cezanne, CGI-70, CYLD, de-ubiquitinating enzyme, deubiquinating isopeptidase T, deubiquitinating enzyme, Doa4, Doa4 ubiquitin hydrolase, Doa4p ubiquitin isopeptidase, DUB, dUCH, EC 3.1.2.15, esterase, ubiquitin thiol-, hydrolase, ubiquitin carboxyl-terminal, hydrolase, ubiquitin carboxyl-terminal (Aplysia californica gene Ap-uch), Iso-T, isopeptidase, isopeptidase T, More, Neuron cytoplasmic protein 9.5, neuronal-specific protein gene product 9.5, OsUCH1, OsUCH2, OsUCH3, OsUCH4, OsUCH5, OTU-1, OTUB1, OTUB2, Otubain-1, Otubain-2, ovarian tumour 1, PA-700 associated isopeptidase, papain-like protease, PGP 9.5, PGP 9.5/UCHL1, PGP9.5, PGP9.5.1, PLP2, PLpro, Ub isopeptidase, ubiquitin C terminal hydrolase 1, ubiquitin C-terminal hydrolase, ubiquitin C-terminal hydrolase (Aplysia californica gene Ap-uch), ubiquitin C-terminal hydrolase 1, ubiquitin C-terminal hydrolase 37, ubiquitin C-terminal hydrolase 8, ubiquitin C-terminal hydrolase isoform L3, ubiquitin C-terminal hydrolase L-1, ubiquitin C-terminal hydrolase L1, ubiquitin C-terminal hydrolase L3, Ubiquitin C-terminal hydrolase UCH37, ubiquitin C-terminal hydrolase-1, ubiquitin C-terminal hydrolase-L1, ubiquitin C-terminal hydrolase-L3, ubiquitin C-terminal hydrolase-L5, ubiquitin C-terminal hydrorase-L1, ubiquitin carboxy terminal hydrolase-L1, ubiquitin carboxy terminal hydrolase-L3, ubiquitin carboxy-terminal hydrolase, ubiquitin carboxy-terminal hydrolase 1, ubiquitin carboxy-terminal hydrolase L1, ubiquitin carboxy-terminal hydrolase-L1, ubiquitin carboxy-terminal hydrolase-L3, ubiquitin carboxyhydrolase L3, ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase 1, ubiquitin carboxyl terminal hydrolase L1, ubiquitin carboxyl terminal hydrolase-L1, ubiquitin carboxyl-terminal hydrolase, ubiquitin carboxyl-terminal hydrolase 1, ubiquitin carboxyl-terminal hydrolase 44, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal hydrolase L-1, ubiquitin carboxyl-terminal hydrolase L1, ubiquitin carboxyl-terminal hydrolase l3, ubiquitin carboxyl-terminal hydrolase L5, ubiquitin carboxyl-terminal hydrolase-L1, ubiquitin carboxyl-terminal hydrolase-L5, ubiquitin carboxyterminal hydrolase L1, ubiquitin carboxyterminal hydrolase L3, ubiquitin carboxyterminal hydrolase-L1, ubiquitin hydrolase, ubiquitin hydrolase Uch-L1, ubiquitin hydrolase UCH-L3, ubiquitin isopeptidase, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, Ubiquitin thiolesterase L3, Ubiquitin thiolesterase L4, Ubiquitin thiolesterase L5, ubiquitin-C-terminal hydrolase L1, ubiquitin-carboxyl-terminal hydrolase PGP-9.5, ubiquitin-specific protease 44, UBPY, UCH, UCH L-1, UCH L1, UCH-1, UCH-8, UCH-L1, UCH-L2, UCH-L3, UCH-L4, UCH-L5, UCH37, UCH54, UCHL-1, UCHL-3, UCHL1, UCHL1/PGP 9.5, UCHL2, Uchl3, UCHL5, UCHL5N240, USP19, USP22, USP44, yeast ubiquitin hydrolase, YUH, YUH1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.12 ubiquitinyl hydrolase 1

Purification

Purification on EC 3.4.19.12 - ubiquitinyl hydrolase 1

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
175fold
-
4 isozymes
-
affinity purified
BPLF1 1-246 and BPLF1 C61S purified by immunoprecipitation
by anion exchange chromatography and gel filtration
-
by gel filtration
by Ni-NTA affinity chromatography, to more than 90% homogeneity
-
by two purification steps, gel filtration
fusion proteins purified by ProBond purification system
isozyme ISOT-S from erythrocytes
-
native isozymes UCHL1-L3 from thymus by anion exchange chromatography and gel filtration, isozyme UCHL1 to homogeneity after this step. PGP 9.5 purified from bovine brain, isozyme UCHL3 is further specifically purified by ubiquitin affinity chromatography
-
on Ni-NTA column
on Ni-NTA resin
-
partial
partial, recombinant wild-type and mutant isozymes ISOT-S and ISOT-L from Escherichia coli
-
purification of GST-tagged UCH-L1 by gel filtration
-
recombinant
recombinant GST-tagged enzyme from Escherichia coli
-
recombinant GST-tagged UCH-L1 from Escherichia coli strain Rosetta BL21(DE3) by glutathione affinity chromatography
recombinant GST-tagged wild-type UCHL1 and mutant S18Y from Escherichia coli strain BL21(Rosetta) by glutathione affinity chromatography, cleavage of the tag, and gel filtration
recombinant His-tagged wild-type and mutants of isozyme L1 expressed in Escherichia coli
recombinant His-tagged wild-type and mutants of isozyme L1 expressed in Escherichia coli DH5alphaPRO
recombinant N-terminally GST-tagged UCH37 catalytic domain from Escherichia coli BL21(RIPL) by glutathione affinity chromatography, proteolytic removal of the GST-tag, and gel filtration
recombinant truncated GST-fusion protein of UBPY
recombinant UCH purified to homogeneity by gel filtration, fusion protein purified by Ni-NTA chromatography
-
recombinant wild-type and mutant GST-tagged enzymes from Escherichia coli by glutathione affinity chromatography and gel filtration
recombinant wild-type and mutants of isozymes L1 and L3 from Escherichia coli BL21(DE3), to near homogeneity
-
UCH-8, to homogeneity
-
UCH-L1, UCH-L2, UCH-L3
-
UCH-L1, UCH-L3
-
UCH-L3 purified on Ni-NTA column
wild-type and mutant Bap1-flag proteins immunopurified with alpha-flag beads