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3.4.19.12: ubiquitinyl hydrolase 1

This is an abbreviated version!
For detailed information about ubiquitinyl hydrolase 1, go to the full flat file.

Word Map on EC 3.4.19.12

Reaction

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) =

Synonyms

A20, AD-019, AMSH, associated molecule with the SH3-domain of STAM, AT-3, ataxin-3, BAP1, BPLF1, BRCA1-associated protein-1, C-terminal hydrolases UCHL3, C-terminal ubiquitin hydrolase, Cezanne, CGI-70, CYLD, de-ubiquitinating enzyme, deubiquinating isopeptidase T, deubiquitinating enzyme, Doa4, Doa4 ubiquitin hydrolase, Doa4p ubiquitin isopeptidase, DUB, dUCH, EC 3.1.2.15, esterase, ubiquitin thiol-, hydrolase, ubiquitin carboxyl-terminal, hydrolase, ubiquitin carboxyl-terminal (Aplysia californica gene Ap-uch), Iso-T, isopeptidase, isopeptidase T, More, Neuron cytoplasmic protein 9.5, neuronal-specific protein gene product 9.5, OsUCH1, OsUCH2, OsUCH3, OsUCH4, OsUCH5, OTU-1, OTUB1, OTUB2, Otubain-1, Otubain-2, ovarian tumour 1, PA-700 associated isopeptidase, papain-like protease, PGP 9.5, PGP 9.5/UCHL1, PGP9.5, PGP9.5.1, PLP2, PLpro, Ub isopeptidase, ubiquitin C terminal hydrolase 1, ubiquitin C-terminal hydrolase, ubiquitin C-terminal hydrolase (Aplysia californica gene Ap-uch), ubiquitin C-terminal hydrolase 1, ubiquitin C-terminal hydrolase 37, ubiquitin C-terminal hydrolase 8, ubiquitin C-terminal hydrolase isoform L3, ubiquitin C-terminal hydrolase L-1, ubiquitin C-terminal hydrolase L1, ubiquitin C-terminal hydrolase L3, Ubiquitin C-terminal hydrolase UCH37, ubiquitin C-terminal hydrolase-1, ubiquitin C-terminal hydrolase-L1, ubiquitin C-terminal hydrolase-L3, ubiquitin C-terminal hydrolase-L5, ubiquitin C-terminal hydrorase-L1, ubiquitin carboxy terminal hydrolase-L1, ubiquitin carboxy terminal hydrolase-L3, ubiquitin carboxy-terminal hydrolase, ubiquitin carboxy-terminal hydrolase 1, ubiquitin carboxy-terminal hydrolase L1, ubiquitin carboxy-terminal hydrolase-L1, ubiquitin carboxy-terminal hydrolase-L3, ubiquitin carboxyhydrolase L3, ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase 1, ubiquitin carboxyl terminal hydrolase L1, ubiquitin carboxyl terminal hydrolase-L1, ubiquitin carboxyl-terminal hydrolase, ubiquitin carboxyl-terminal hydrolase 1, ubiquitin carboxyl-terminal hydrolase 44, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal hydrolase L-1, ubiquitin carboxyl-terminal hydrolase L1, ubiquitin carboxyl-terminal hydrolase l3, ubiquitin carboxyl-terminal hydrolase L5, ubiquitin carboxyl-terminal hydrolase-L1, ubiquitin carboxyl-terminal hydrolase-L5, ubiquitin carboxyterminal hydrolase L1, ubiquitin carboxyterminal hydrolase L3, ubiquitin carboxyterminal hydrolase-L1, ubiquitin hydrolase, ubiquitin hydrolase Uch-L1, ubiquitin hydrolase UCH-L3, ubiquitin isopeptidase, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, Ubiquitin thiolesterase L3, Ubiquitin thiolesterase L4, Ubiquitin thiolesterase L5, ubiquitin-C-terminal hydrolase L1, ubiquitin-carboxyl-terminal hydrolase PGP-9.5, ubiquitin-specific protease 44, UBPY, UCH, UCH L-1, UCH L1, UCH-1, UCH-8, UCH-L1, UCH-L2, UCH-L3, UCH-L4, UCH-L5, UCH37, UCH54, UCHL-1, UCHL-3, UCHL1, UCHL1/PGP 9.5, UCHL2, Uchl3, UCHL5, UCHL5N240, USP19, USP22, USP44, yeast ubiquitin hydrolase, YUH, YUH1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.12 ubiquitinyl hydrolase 1

Cloned

Cloned on EC 3.4.19.12 - ubiquitinyl hydrolase 1

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
active C-terminal His-tagged UCH expressed in Pichia pastoris
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ataxin-3 carrying six consecutive glutamines cloned into plasmid pGEX6P-1 and expressed as a GST fusion protein in the Escherichia coli Codon Plus-RIL strain
BPLF1 N-terminal fragment containing the first 246 amino acids cloned into the pFLAG-CMV-2 vector. Expression of the fragment or mutant in HEK-293T cells
co-expression of Doa4p with cyk-3 of Caenorhabditis elegans and ubiquitin-beta-galactosidase fusion protein in Escherichia coli
-
COS-7 cells co-transfected with plasmids encoding His-tagged UCH-L1 and FLAG-tagged ubiquitin
-
determination of the UCHL1 promoter DNA methylation status and the mechanisms for the heterogeneous UCHL1 expression pattern in renal epithelium and in renal cell carcinoma, overview. Semi-quantitative and real-time RT-PCR analysis
-
expressed by the pCold TF cold shock expression system in Escherichia coli
expressed in BAEC cells
-
expressed in COS-7 cells
expressed in Escherichia coli
-
expressed in KR-4 cells
-
expressiom of GFP-tagged wild-type UCH L1 and mutants in PC12 Tet-off cells and in CHO-AA8-Luc1 cells
-
expression in Escherichia coli as GST-tagged enzyme
-
expression of a catalytically active UCH-L1 in a UCH-L1 negative EBV transformed lymphoblastoid cell line
expression of EGFP-tagged UCHL1 in oocytes
-
expression of GST-tagged UCH-L1 in Escherichia coli strain Rosetta BL21(DE3)
expression of GST-tagged wild-type and mutant enzymes in HEK-293 cells
expression of GST-tagged wild-type UCHL1 and mutant S18Y in Escherichia coli strain BL21(Rosetta)
expression of HA-tagged wild-type and mutant enzymes in HEK-293 cells
-
expression of His-tagged wild-type and mutant enzymes
-
expression of UCH-L1 in H1299 cells
-
expression of wild-type and hydrolase-deficient mutant UCH-L3 in mouse embryonic finbroblasts
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expression vectors encoding HA epitope-tagged Cezanne (pHM6-Cezanne), GFP-tagged Cezanne (pEGFP-Cezanne) and enzymatically inactive forms mutated at the catalytic cysteine (pHM6-Cezanne C209S and pEGFP-Cezanne C209S). Expressed in HEK293 cells
-
FLAG-HA tagged UCH-3 expressed in HeLa cells
FLAG-tagged UCH-L1 overexpression in the SH-SY5Y human neuroblastoma cell line, which endogenously expresses UCH-L1 and alpha-synuclein, can be cytotoxic at high expression levels in these cells. Coexpression of wild-type UCH-L1, but not of the protective S18Y variant, in COS-7 cells increases levels of transfected alpha-synuclein by inhibiting its degradation, the amount of UCH-L1M in transfected cells correlates with the intracellular level of alpha-synuclein
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Flag-tagged wild-type or mutant Bap1 expressed in HEK-293 cells. HA-tagged Bap1 transiently expressed in HeLa cells
Flag-USP19 transfected into FR3T3 cells. 293VSV cells transfected with pLXSN plasmid encoding N-terminally Flag3-tagged USP19
gene cyk-3, chromosome mapping, co-expression of cyk-3 with Doa4p of Saccharomyces cerevisiae and ubiquitin-beta-galactosidase fusion protein in Escherichia coli
-
gene rs5030732, genotyping
gene rs5030732, genotyping and UCHL1 polymorphisms, expression analysis, overview
-
gene uch-L1, DNA and amino acid sequence determination and analysis
gene uch-L1, DNA and amino acid sequence determination and analysis, determination and cloning of the promoter sequence, promoter activity and expression analysis, overexpression of the gene and promoter in zebrafish embryos by microinjection
gene uchl1, phylogenetic analysis
gene UCHL5, DNA and amino acid sequence determination and analysis
gene usp21, DNA sequence determination and analysis, located on chromosome 1q21, expression in COS cells, overexpression in U2O2 cells
HEK-293 cells transiently transfected with FLAG (DYKDDDDK)-tagged UCH37 expression construct
-
high-resolution X-ray crystal structure of the enzyme (PLP2) in complex with ubiquitin
human UCHL1 and bovine eNOS subcloned into D-TOPO and transferred into the mammalian expression vector pcDNA3.1 or pCAGGS, transfection of plasmid DNA in rat carotid artery
-
into the pcDNA3.1-TOPO vector and expressed in HCT-116 or EC-109 cells
into vector pcDNA3.1(+) in frame with a C-terminal HA epitope tag and expressed in Escherichia coli
-
isozyme L1: expression of wild-type and mutants in Escherichia coli as His-tagged proteins
isozyme L1: expression of wild-type and mutants in Escherichia coli DH5alphaPRO as His-tagged proteins
isozymes ISOT-S and ISOT-L, cloning from brain mRNA, expression of both isozyme wild-types and mutant isozyme ISOT-S C335S in Escherichia coli strain MC1061
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overexpression of isozyme L1 in mouse embryonic fibroblasts via adenovirus transfection
overexpression of wild-type and mutant GST-tagged enzymes in Escherichia coli
overexpression of wild-type and mutant isozymes L1 and L3 in Escherichia coli BL21(DE3)
-
pcDNA3.1-UCH-L1 plasmid overexpressed in MCF7 and MCF7/Adr cells
plasmids containing wild-type UCH-L1 and mutants with or without FLAG tag transiently transfected into Neuro2a, SH-SY5Y or COS-7 cells. NIH-3T3 cells stably expressing human UCH-L1 with a FLAG-HA double tag at the N-terminus. PGEX UCH-L1 vectors transformed into Escherichia coli BL21
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PLP2 domain originating from PEDV polyprotein 1a is transformed into Escherichia coli BL21 (DE3) competent cells
recombinant fusion proteins expressed in Escherichia Coli BL21(DE3)pLysS cells
recombinant UCH37 catalytic domain as N-terminally GST-tagged protein is expressed in Escherichia coli BL21(RIPL)
stable expression of HA-tagged wild-type UCH-L1 in murine NIH-3T3 cells, expression of FLAG-tagged or GFP-tagged wild-type UCH-L1 and mutants D30A and C90S in murine neural progenitor NP cells, the expression level is lower in NP cells derived from gad, i.e. gracile axonal dystrophy, mice compared to NP cells from wild-type mice, overview
-
subcloned from pcDNA-UCH-L1 vector into pGEX-6P-1 vector. The resulting N-terminally fused GST-tagged protein expressed in Escherichia coli Rosetta strain
two plasmids with two types of YUH1 with the 6 histidine tag at the N-terminus (H6YUH1) or C-terminus (YUH1H6) expressed in Escherichia coli DH5alpha
-
UBPY, DNA sequence determination and analysis, expression of gene fragment, residues 9-188, fused to GST in a bacterial expression system
UBPY, expression of wild-type enzyme and mutant D786A in NIH 3T3 cells
UCH-L1 expression analysis by microarray, quantitative real time-PCR
-
UCH-L1, UCH-L3
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UCH-L3 subcloned into a pET28 expression vector bearing an N-terminal histidine tag. Full-length UCH-L3 expressed in Escherichia coli BL21(DE3) cells
Uch37N (amino acids 1-237) expressed from pET21a with a C-terminal 6× histidine tag in Escherichia coli BL21(DE3). HEK293 cells stably expressing Uch37 containing an amino-terminal 2 × HA epitope. Uch37 expressed as a carboxy- or amino-terminal fusion to the Gal4 activation domain or the Gal4 DNA-binding domain. GST-Uch37, GST-Uch37N (1-237), GST-Uch37C (238-329), wild-type 6× His-Flag-Uch37 and mutant C88A subcloned into pBacPAK8 with their respective epitope tags and expressed using the BacPAK baculovirus expression system, expressed in Sf21 insect cells. DNA encoding human Uch37FL, Uch37N (1-237), Adrm1, and the 19S complex subunits S1, S2, S3, S4, S5a, S5b, S6, S6’, S7, S8, S9, S10a, S10b, S11, S12, S13, S14, S15 and p55 subcloned into pGAD or pGBD yeast two-hybrid vectors and transformed into the YRG-2 yeast strain
UCHL1 gene, the gene and its proximal promoter with a classic TATA box 25 bp upstream of a G base determined to be the transcription start site from mRNA 5'-mapping analyses, gene ragulation, detailed overview
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YUH gene ligated with the pET-23d vector, expressed in Escherichia coli Rosetta(DE3)pLysS