3.4.11.B4: Pyrococcus horikoshii deblocking aminopeptidase

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For detailed information about Pyrococcus horikoshii deblocking aminopeptidase, go to the full flat file.

Reaction

The enzyme exhibits aminopeptidase activity and with lower efficiency deblocking activity (i.e. hydrolysis of acetylated amino acids from the N-terminus of peptides). The turnover number with Ac-Ala-Ala-Ala is 260-540fold lower compared to the activity with Ala-Ala-Ala. The turnover number with Ac-Ala-Ala is 350fold lower compared to the activity with Ala-Ala. Low hydrolytic activity for peptide substrates longer than 10 residues. The enzyme is activated by Co2+ or Zn2+. Contains 1.32 mol of Ca2+ per mol of monomer. =

Synonyms

DAP1, DAP2, DAP3, DAPPh, DAPPh2, DAPPh3, deblocking aminopeptidase, PH0519, PH1527, PH1821

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.11 Aminopeptidases

                3.4.11.B4 Pyrococcus horikoshii deblocking aminopeptidase

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Results	in table
3	AA Sequence
4	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Information
2	Inhibitors
8	KM Value [mM]
6	Metals/Ions
6	Molecular Weight [Da]
7	Organism
3	pH Optimum
3	pH Range
3	Protein Variants
4	Purification (Commentary)
1	Reaction
4	Reference
17	Substrates and Products (Substrate)
4	Subunits
19	Synonyms
4	Temperature Optimum [°C]
3	Temperature Range [°C]
4	Temperature Stability [°C]
9	Turnover Number [1/s]

Sequence

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Sequence on EC 3.4.11.B4 - Pyrococcus horikoshii deblocking aminopeptidase

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTIONThe reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

O58255 pBLAST

O58255_PYRHO

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

332

0

36924

TrEMBL

Show Sequence

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O59196 pBLAST

TET_PYRHO

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

353

0

39014

Swiss-Prot

Show Sequence

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O59485 pBLAST

O59485_PYRHO

Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

354

0

39008

TrEMBL

Show Sequence

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