3.4.11.25: beta-peptidyl aminopeptidase
This is an abbreviated version!
For detailed information about beta-peptidyl aminopeptidase, go to the full flat file.
Word Map on EC 3.4.11.25
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3.4.11.25
-
synthesis
-
beta-amino
-
beta-peptides
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alpha-amino
-
rrna
-
l-configuration
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preproteins
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aminopeptidases
-
anthropi
-
ochrobactrum
- 3.4.11.25
- synthesis
-
beta-amino
- beta-peptides
-
alpha-amino
- rrna
-
l-configuration
- preproteins
- aminopeptidases
-
anthropi
-
ochrobactrum
Reaction
cleaves N-terminal beta-homoamino acids from peptides composed of 2 to 6 amino acids =
Synonyms
3-2W4 BapA, BapA, BapF, PA1486, Y2 BapA
ECTree
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Substrates Products
Substrates Products on EC 3.4.11.25 - beta-peptidyl aminopeptidase
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REACTION DIAGRAM
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
2 beta-homoGly 4-nitroanilide
beta-homoGly-beta-homoGly 4-nitroanilide + 4-nitroaniline
-
product of mutant S237C, synthesized efficiently without degradation
-
?
beta-homoGly 4-nitroanilide + H2O
beta-homoGly + 4-nitroaniline
-
wild-type enzyme shows beta-homoGly-beta-homoGly-4-nitroanilide synthetic activity, but the product vanishes in a few min and converts to free beta-homoGly
-
?
beta-homoVal-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
insulin + rac-3-aminobutanamide
?
enatioselectivity for beta-alanine is more than 300:1
-
-
r
Val-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
only the N-terminal beta-homoVal is released. Preferred substrate
-
-
?
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
beta-Ala + Ile-beta-homoTyr
1.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
1.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala + L-His
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
-
-
-
?
beta-homoAla + beta-homoLeu
preferred substrate
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
preferred substrate
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoArg + Ile-beta-homoTyr
3.4% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoGln + Ile-beta-homoTyr
0.3% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoHis + Ile-beta-homoTyr
0.4% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoLeu + Ile-beta-homoTyr
12% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
61% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoLys + Ile-beta-homoTyr
0.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
0.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoPhe + Ile-beta-homoTyr
15% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
22% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoSer + Ile-beta-homoTyr
13% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoThr + Ile-beta-homoTyr
1.6% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoTrp + Ile-beta-homoTyr
1.3% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
1.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoTyr + Ile-beta-homoTyr
7% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
15% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal + beta-homoAla-beta-homoLeu
27% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
preferred substrate
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal + beta-homoArg-beta-homoArg
hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal + beta-homoIle + beta-homoTyr
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal + beta-homoIle + Tyr
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr
-
-
?
beta-homoVal + Ile-beta-homoTyr
15% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
32% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
D-beta-homoVal + Ile-beta-homoTyr
hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
the rate of cleavage is slower by factor 28 as compared to the L-enantiomer. 0.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
the rate of cleavage is slower by factor 35 as compared to the L-enantiomer. 0.9% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
-
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
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in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
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additional information
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the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
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