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3.2.2.22: rRNA N-glycosylase

This is an abbreviated version!
For detailed information about rRNA N-glycosylase, go to the full flat file.

Word Map on EC 3.2.2.22

Reaction

A4324 in 28S rRNA
+
H2O
=
deadenylated 28S rRNA
+
adenine

Synonyms

(ribosome-inactivating protein)-like protein, abelesculin, ABRaA, abrin, abrin-a A chain, Abrus precatorius agglutinin, alpha-MC, alpha-momorcharin, alpha-pisavin, antiviral protein DAP-30, aralin, B-32, balsamin, BBAP1, BE27, beta-momorcharin, beta-pisavin, Bougainvillea xbuttiana antiviral protein 1, bouganin, camphorin, CAP30, cinnamomin, Cochinin B, cytotoxic ribosome-inactivating lectin, D6D54_07625, D6D54_08385, D6D54_08880, depurinating rRNA N-glycosidase, dianthin 30, dianthin 32, dianthin-30, diphtheria toxin, ebulin b, ebulitin alpha, ebulitin beta, ebulitin gamma, gelonin, gysophilin, hispin, IRAb, IRIP, Iris agglutinin b, Iris ribosome-inactivating protein, JIP60, karasurin-A, KML, lamjapin, luffaculin I, luffin P1, lychnin, MAP, marmorin, ME1, Mirabilis antiviral protein, mistletoe lectin I, mistletoe lectin II, mistletoe lectin III, ML-I, MLI, MLII, MLIII, MOD, modeccin, momorcochin, momorcochin-S, momordin, momordin I, momordin II, momorgrosvin, More, N-glycosidase, n-TCS, neo-trichosanthin, nigrin b, nigritin f1, nigritin f2, PAG, PAGase, PAP, PAP-I, PAP-II, PAP-III, PAP-R, PAP-S, PD-L, PD-L1, PD-L4, pepocin, PGase, pokeweed anti-viral protein, pokeweed antiviral protein, polynucleotide:adenosine glycosidase, polynucleotide:adenosine glycosylase, porrectin, RA, RCA-II, ribosomal inactivating protein, ribosomal ribonucleate N-glycosidase, ribosome inactivating protein, ribosome-inactivating protein, ribosome-inactivating protein 3, ribosome-inactivating protein type II, ribosome-inactivating proteins, ribosome-inactivating type II protein, ribosome-specific N-glycosidase, ricin, ricin A, ricin A chain, ricin A-chain, ricin toxin A-chain, ricin-A-chain, ricin-like protein, Ricinus communis agglutinin, Ricinus communis agglutinin II, RIP, RIP-1, RIP1, RIP2, RIPII, riproximin, RLP1, RLP2, RLP3, RLP4, RLP5, RLP6, RNA N-glycosidase, RNA-NGA, RNA-specific N-glycosidase, RNase Bo, Rpx, rRNA N-glycosidase, RTA, Sambucus nigra agglutinin, Sambucus nigra agglutinin I, SAP, saporin, saporin 6, saporin S-6, saporin S6, saporin-6, saporin-L1, saporin-S6, saporins, SBA, SCO7092, SEPIN, Shiga toxin, Shiga toxin 1, Shiga toxin 1 subunit A, Shiga toxin 2, Shiga toxin 2 subunit A, Shiga toxin type 1, Shiga toxin type 2, Shiga toxin-1, shiga-like toxin, shiga-like toxin I, sieboldin-b, single-chain ribosome-inactivating protein, SLT-I, SLT-II, SLT-IIv, SNA, SNA-I, SNA-V, SnaI, SNAIf, SNAV, SNLRP, SoRIP1, SoRIP2, stenodactylin, Stx, Stx type 1, Stx-1, Stx1, Stx1A, Stx2, Stx2A, Stx2dact, StxB, TCS, trichoanguin, trichomaglin, trichosanthin, Trichosanthrip, trichosnathin, TRIP, tritin-L, tritin-S, TYchi, type 1 ribosome inactivating protein, type 1 ribosome-inactivating protein, type 1 RIP, type 2 ribosome inactivating protein, type 2 ribosome-inactivating protein, type 2 RIP, type 2RIP, type 3 RIP, type I ribosome inactivating protein, type I ribosome-inactivating protein, type I RIP, type II ribosome inactivating protein, type II ribosome-inactivating protein, type II RIP, type III ribosome-inactivating protein, type III RIP, type-1 ribosome-inactivating protein, type-1 RIP, type-2 ribosome-inactivating protein, type-2 RIP, VAA-I, viscinum, Viscum album agglutinin I, viscumin, volkensin

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.22 rRNA N-glycosylase

Renatured

Renatured on EC 3.2.2.22 - rRNA N-glycosylase

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
coarse-grained latice simulation model of sequence-structure modifications of the ricin A chain protein fold. Calculation of unfolding-folding transition temperature and evaluation of a possible unfolding model
-
in vitro reconstitution of volkensin from separately expressed A and B chain in presence of a GSH/GSSH reduced/oxidized system
-
reassociation of purified recombinant RTAs mixed with RTB in presence of 0.1 M lactose and 2% v/v 2-mercaptoethanol by dialysis for 24 h against 1 l of NaCl/phosphate solution containing 0.1 M lactose, followed by further 36 h against 5 l of NaCl/phosphate solution
-
recombinant B-subunit from Escherichia coli strain BL21(DE3) inclusion bodies by denaturation in 7 M guanidinium hydrochloride, renaturation by 130fld dilution in phosphate buffer, pH 7.4, and incubation for 16 h at 4°C
-
recombinant CBD-intein-Gel fusion protein expressed in Escherichia coli strain Bl21(DE3) from inclusion bodies by denaturation and stepwise dialysis, 69% of the protein is refolded to the native state in presence of 0.02 mM GSSG and 0.2 mM GSH in 10 mM Tris-HCl, 0.5 M NaCl, pH 8.5, and 0.5 M L-arginine
-
recombinant functional His6-tagged enzyme from Escherichia coli inclusion bodies, method, overview
-
renaturation of recombinant MLIII B-subunit from 7 M guanidinium hydrochloride by dilution with phosphate buffer, pH 7.4, and incubation for 16 h at 4°C
-
study on conformation of partially unfolded state through steady-state and time-resolved fluorescence spectroscopy on a single Trp-190 which is located adjacently to the active site. The transient folding/unfolding intermediate state of beta-momorcharin adopts a specific conformation at the vicinity of the active site
-
the recombinant secreted ricin-B expressed in tobacco hairy root culture is degraded in the medium within 24 h with complete loss after 72 h, the recombinant ricin-B can be recovered by aqueous two-phase extraction using PEG/potassium phosphate or PEG/sodium sulfate, the latter is more efficient
-