affinity column chromatography with a 10 x 2.5 cm column packed with amylose resin specific for maltose-binding protein, eluted pure protein cleaved and dialyzed against 20 mM sodium acetate, pH 5.2, with 0.01% sodium azide, loaded to Sephadex G-100 column
cell centrifugation, washing, lysozyme application, sonication, centrifugation, supernatants applied to GSTrap FF column, elution with sonication buffer and 10 mM reduced glutathione, desalted, concentrated and glutathione S-transferase (GST) cleaved off with PreScission Protease
centrifugation of homogenized fruiting bodies followed by ion exchange chromatography on a diethylaminoethyl-cellulose column in 10 mM Tris-HCl buffer, pH 7, Affi-gel blue gel column chromatography of unabsorbed protein fraction (with translation-inhibiting activity) in 10 mM Tris-HCl buffer, pH 7 yields bound marmorin
centrifugation, 0.22 microM filtered, dialyzed against 25 mM sodium chloride in phosphate buffer, pH 6.0, with 2 mM EDTA, application to HP CM FF Sepharose ion exchange column
homogenized frozen leaves filtered through cheesecloth, centrifuged, saturated with ammonium sulfate to 60%, stirred, all at 4 degrees Celsius, centrifuged, pellet resuspended in 20 mM Tris-HCl, pH 7.5 with 1 mM beta-mercaptoethanol, and 0.2 mM EDTA, dialyzed against buffer, centrifuged, loaded onto HiTrap Q FF column, bound proteins loaded onto HiTrap SP FF column, eluted with NaCl gradient, PAP elutes at about 250 mM NaCl
recombinant His6-tagged ME1 from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography, using a polyclonal anti-ME1 antibody resin, and anion exchange chromatography
recombinant isozymes from Escherichia coli strain BL21(DE3), purification after overexpression affords refolding by glutathione in refolding buffer, followd by ultrafiltration and cation exchange chromatography
recombinant refolded CBD-intein-Gel fusion protein from inclusion bodies by chitin affinity chromatography with elution using an intein C-terminal self-cleavage method, 96% purity
recombinant ricin A-chain RTA V81M without N- and C-terminal extensions from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and anion exchange chromatography
recombinant SNA-I, SNA-IV, SNA-V, and SNLRP from Nicotiana tabacum cv. Samsun NN transgenic plants by ion exchange chromatography, and fetuin affinity chromatography for SNA-I, recombinant SNA-IV and SNA-V are purified by ammonium sulfate fractionation, galactose affinity chromatography, and hydrophobic interaction chromatography, recombinant SNLRP is purified by ion exchange chromatography and gel filtration
recombinant wild-type and mutant ricin A chains from Escherichia coli strain JM101 by anion exchange chromatography and cobalt affinity chromatography to homogeneity
sonicated, cleared lysate of cell culture in 20 mM Tris buffer, pH 8.0 with 100 mM NaCl, 1 mM Na-vanadate and protease inhibitors (GST-buffer), incubated with glutathione-Sepharose beads, transferred to column, washed with 0.1 M Tris buffer, pH 7.8 with 0.5 M NaCl, with GST-buffer containing 0.35% Triton X-100, and GST-buffer alone
cell centrifugation, washing, lysozyme application, sonication, centrifugation, supernatants applied to GSTrap FF column, elution with sonication buffer and 10 mM reduced glutathione, desalted, concentrated and glutathione S-transferase (GST) cleaved off with PreScission Protease
cell centrifugation, washing, lysozyme application, sonication, centrifugation, supernatants applied to GSTrap FF column, elution with sonication buffer and 10 mM reduced glutathione, desalted, concentrated and glutathione S-transferase (GST) cleaved off with PreScission Protease
3.2.2.22 rRNA N-glycosylase
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