3.2.1.B33: Sulfolobus shibatae beta-glycosidase
This is an abbreviated version!
For detailed information about Sulfolobus shibatae beta-glycosidase, go to the full flat file.
Reaction
Hydrolysis of beta-glycosidic bonds with broad specificity. It prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) =
Synonyms
Sulfolobus shibatae beta-glycosidase
ECTree
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Temperature Stability
Temperature Stability on EC 3.2.1.B33 - Sulfolobus shibatae beta-glycosidase
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70
-
the enzyme retains 50% of the initial activity towards 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside after 146 h and 127 h of incubation in phosphate (pH 7.2) or acetate (pH 5.5) buffers. The half-lifes of the enzyme activity at this temperature are decreased after preincubation in acetate buffer (pH 5.5) up to 32 and 27 h, respectively
80
-
5 h, in 0.1 M acetate buffer (pH 5.5), the enzyme retained about 67% of the initial activity