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3.2.1.B33: Sulfolobus shibatae beta-glycosidase

This is an abbreviated version!
For detailed information about Sulfolobus shibatae beta-glycosidase, go to the full flat file.

Reaction

Hydrolysis of beta-glycosidic bonds with broad specificity. It prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc) =

Synonyms

Sulfolobus shibatae beta-glycosidase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.B33 Sulfolobus shibatae beta-glycosidase

Temperature Stability

Temperature Stability on EC 3.2.1.B33 - Sulfolobus shibatae beta-glycosidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
the enzyme retains 50% of the initial activity towards 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside after 146 h and 127 h of incubation in phosphate (pH 7.2) or acetate (pH 5.5) buffers. The half-lifes of the enzyme activity at this temperature are decreased after preincubation in acetate buffer (pH 5.5) up to 32 and 27 h, respectively
75
-
half-life: 15 h
80
-
5 h, in 0.1 M acetate buffer (pH 5.5), the enzyme retained about 67% of the initial activity
95
-
half-life: 3.9 min
98
-
half-life at pH 7.2: 54 min, half-life at pH 5.5: 30 min