3.2.1.173: rhamnogalacturonan galacturonohydrolase

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For detailed information about rhamnogalacturonan galacturonohydrolase, go to the full flat file.

Reaction

Exohydrolysis of the alpha-D-GalA-(1->2)-alpha-L-Rha bond in rhamnogalacturonan oligosaccharides with initial inversion of configuration releasing D-galacturonic acid from the non-reducing end of rhamnogalacturonan oligosaccharides =

Synonyms

RG-galacturonohydrolase, RGGH, RGI exo-hydrolase, RGI galacturonohydrolase, RGI hydrolase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.173 rhamnogalacturonan galacturonohydrolase

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1	Cloned(Commentary)
1	General Information
3	kcat/KM [mM/s]
5	KM Value [mM]
1	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
3	Organism
2	pH Optimum
1	pH Range
3	pH Stability
2	pI Value
1	Purification (Commentary)
2	Reaction
3	Reference
2	Specific Activity [micromol/min/mg]
11	Substrates and Products (Substrate)
2	Subunits
5	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.2.1.173 - rhamnogalacturonan galacturonohydrolase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha + H2O

beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha

Aspergillus aculeatus

-

-

710299

-

-

?

alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha + H2O

beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha

Aspergillus aculeatus

-

-

710299

-

-

?

alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]4-(1->2)-alpha-L-Rha + H2O

beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]4-(1->2)-alpha-L-Rha

Aspergillus aculeatus

-

-

710299

-

-

?

alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha + H2O

beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha

Aspergillus aculeatus

-

-

710299

-

-

?

alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]6-(1->2)-alpha-L-Rha + H2O

beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]6-(1->2)-alpha-L-Rha

Aspergillus aculeatus

-

-

710299

-

-

?

MHR-S + H2O

D-galactose + ?

Aspergillus aculeatus

-

i.e. saponified modified hairy regions of rhamnogalacturonan I, from apple pectin, residue remaining after enzymatic liquefaction of pectin

754912

-

-

?

rhamnogalacturonan I + H2O

?

Aspergillus aculeatus

-

RGI, degrading enzymes are active on the RGI backbone of pectin and are thus strictly specific for cleaving bonds in the repetitive [(1->2)-alpha-L-Rhap-(1->4)-alpha-D-GalpA-(1->2)] structure

754912

-

-

?

rhamnogalacturonan I + H2O

D-galactose + ?

Aspergillus aculeatus

-

RGI DP 6

754912

-

-

?

rhamnogalacturonan I oligosaccharides with alpha-D-GalA at the non-reducing end + H2O

beta-D-galacturonic acid + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the reducing end and at the nonreducing en

Aspergillus aculeatus

-

active toward all rhamnogalacturonan types of substrate, except for those with a 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end

710299

-

-

?

additional information

?

-

2 entries

additional information

?

-

Aspergillus aculeatus

-

no activity with: CM-cellulose, crystalline cellulose, xylan from oat spelts, soluble starch, potato arabino-beta-(1,4)-galactan, larchwood arabino-beta-(1,3)/(1,6)-galactan (stractan), linear arabinan, polygalacturonic acid with or without 1 mM CaCl2 added, 4-nitrophenyl-alpha-L-Araf, 4-nitrophenyl-alpha-D-Galp, 4-nitrophenyl-beta-D-Galp, 4-nitrophenyl-alpha-D-Xylp, 4-nitrophenyl-beta-D-Xylp, 4-nitrophenyl-alpha-D-Manp, 4-nitrophenylb-D-Manp, 4-nitrophenyl-alpha-L-Fucp, 4-nitrophenyl-beta-D-Fucp, 4-nitrophenyl-alpha-D-Glcp, 4-nitrophenyl-beta-D-Glcp, 4-nitrophenyl-alpha-L-Rhap, 4-nitrophenyl-beta-D-GlcpA, and 4-nitrophenyl-beta-D-GalpA

710299

-

-

?

additional information

?

-

Aspergillus aculeatus

-

RGI endo-hydrolases, RGHs, attack the RGI backbone randomly in an endo-fashion and catalyse bond cleavage of alpha-(1->2) glycosidic bonds between D-GalpA and L-Rhap. The mechanism involves an inversion of the anomeric C1 configuration in galacturonic acid releasing oligosaccharides with beta-D-GalpA at the reducing end and L-Rhap at the non-reducing end as products. The RGGH type of exo-enzyme cleaves off the terminal nonreducing galacturonosyl residue by catalysing cleavage of alpha-(1->2) glycosidic bonds between D-GalpA and L-Rhap in the non-reducing terminus, releasing single beta-D-GalpA. The RGGH from Aspergillus aculeatus is active towards different types of rhamnogalacturonan I (RGI), for example, pectin or pure RGI, except for the ones with unsaturated GalpA at the nonreducing end. The enzyme displays preference for smaller RGI substrates since it has higher activities towards RGI fragment DP6 than saponified modified hairy regions of rhamnogalacturonan I (MHR-S). Aspergillus aculeatus RGGH shows no activity towards HG sub-strates, for example, polygalacturonic acid or small size GalpA acid oligomers

754912

-

-

?