3.2.1.102: blood-group-substance endo-1,4-beta-galactosidase

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For detailed information about blood-group-substance endo-1,4-beta-galactosidase, go to the full flat file.

Reaction

Synonyms

ABase, blood group A- and B-cleaving endo-beta-galactosidase, blood group A/B antigen-specific endo-beta-galactosidase, blood group antigen-cleaving endo-beta-galactosidase, blood group glycotope-cleaving endo-beta-galactosidase, E-ABase, EABase, endo-beta-galactosidase, endo-beta-galactosidase C, endo-beta-galactosidase DII, EndoGalC, galactosidase, endo-beta-, GH98CBM51, inverting endo-beta-galactosidase, More, Sp3GH98, Sp4GH98

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.102 blood-group-substance endo-1,4-beta-galactosidase

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Results	in table
5	AA Sequence
4	Application
1	CAS Registry Number
9	Cloned(Commentary)
2	Crystallization (Commentary)
1	General Information
8	Inhibitors
6	kcat/KM [mM/s]
14	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
25	Organism
2	PDB ID
4	pH Optimum
1	pH Range
7	Protein Variants
8	Purification (Commentary)
1	Reaction
1	Reaction Type
25	Reference
1	Source Tissue
5	Specific Activity [micromol/min/mg]
57	Substrates and Products (Substrate)
1	Subunits
28	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
6	Turnover Number [1/s]

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Engineering on EC 3.2.1.102 - blood-group-substance endo-1,4-beta-galactosidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D429A

Clostridium perfringens

-

the mutants shows reduced activity compared to the wild type enzyme

702342

D453A

Clostridium perfringens

-

the mutation results in complete loss of activity

702342

E354A

Clostridium perfringens

-

the activity of the E354A mutant with nonactivated natural substrates is 1100fold lower than that of the wild type enzyme, while its activity is only 10fold lower when assayed with 2,4-dinitrophenyl-beta-A-trisaccharide

702342

E467A

Clostridium perfringens

-

the mutants shows slightly reduced activity compared to the wild type enzyme

702342

E506A

Clostridium perfringens

-

the mutation results in complete loss of activity

702342

additional information

2 entries

additional information

Clostridium perfringens

-

mice expressing the enzyme systemically have rough and flaky skin which becomes detectable around 5 days after birth and becomes obscure by 2 weeks after birth. In transgenic mice, proliferating keratinocytes increase approximately 3fold in epidermis compared to wild-type. In transgenic epidermis, the expression domain of cytokeratin 14 increases from 1-2 layers to 4-5 layers and co-expresses with cytokeratin 6 and 10 in the upper layers. Transgenic mice also show delayed development of the barrier function around 8 days postnatal, and acquire the function by 12 days postnatal

680046

additional information

Clostridium perfringens

-

transgenic expression of enzyme in mouse results in dramaitc reduction in the expression of the alphaGal epitope. Mice show abnormal phenotypes, such as occasional death immediately after birth, growth retardation, and transient skin lesions

680050