3.2.1.102: blood-group-substance endo-1,4-beta-galactosidase

This is an abbreviated version!
For detailed information about blood-group-substance endo-1,4-beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.102

3.2.1.102

epitope

xenotransplantation

clostridium

alphagal

perfringens

galalpha1-3gal

xenoantigenic

hyperacute

galalpha1-3galbeta1-4glcnac

alpha-galactosyl

saporin

medicine

analysis

Reaction

GalNAcalpha1-3(Fucalpha1-2)Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-R

Synonyms

ABase, blood group A- and B-cleaving endo-beta-galactosidase, blood group A/B antigen-specific endo-beta-galactosidase, blood group antigen-cleaving endo-beta-galactosidase, blood group glycotope-cleaving endo-beta-galactosidase, E-ABase, EABase, endo-beta-galactosidase, endo-beta-galactosidase C, endo-beta-galactosidase DII, EndoGalC, galactosidase, endo-beta-, GH98CBM51, inverting endo-beta-galactosidase, More, Sp3GH98, Sp4GH98

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.102 blood-group-substance endo-1,4-beta-galactosidase

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Results	in table
5	AA Sequence
4	Application
1	CAS Registry Number
9	Cloned(Commentary)
2	Crystallization (Commentary)
1	General Information
8	Inhibitors
6	kcat/KM [mM/s]
14	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
25	Organism
2	PDB ID
4	pH Optimum
1	pH Range
7	Protein Variants
8	Purification (Commentary)
1	Reaction
1	Reaction Type
25	Reference
1	Source Tissue
5	Specific Activity [micromol/min/mg]
57	Substrates and Products (Substrate)
1	Subunits
28	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
6	Turnover Number [1/s]

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Application on EC 3.2.1.102 - blood-group-substance endo-1,4-beta-galactosidase

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analysis

Citrobacter freundii

endo-beta-galactosidase-Griffonia-simplicifolia-agglutinin-II-staining procedure into carbohydrate histochemistry of human tissues. The procedure may provide a powerful tool for analysing the backbone structures carrying the blood-group-related antigens in tissue sections

208411

medicine

2 entries

additional information

unidentified

a highly sensitive fluorescence-based high-throughput assay for both endo-beta-galactosidase and activity specific for the oligosaccharide structure of the blood group A antigen is developed. The potential to use this assay is demostrated to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes

750707