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1-kestotriose + sucrose
1,6-kestotetraose + ?
-
1-kestotriose is the preferred substrate
-
-
?
2 sucrose
6-kestose + D-glucose
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
6G-kestotriose + sucrose
6-kestotriose + ?
-
-
-
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
D-raffinose
D-fructose + melibiose
-
-
-
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
stachyose + (2,6-beta-D-fructosyl)n
?
-
-
-
-
?
sucrose
levan + fructooligosaccharides
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
sucrose + ?
polyfructan + ?
sucrose + acarbose
D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
sucrose + arabinose
?
-
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
sucrose + cellobiose
?
-
-
-
-
?
sucrose + cellobiose
beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
sucrose + cellobiose
D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + cellobiose
fructosylated cellobiose + ?
sucrose + D-arabinose
?
-
-
-
-
?
sucrose + D-fucose
beta-D-fructofuranosyl-alpha-D-fucopyranoside + D-glucose
sucrose + D-galactose
?
-
-
-
-
?
sucrose + D-galactose
beta-D-fructofuranosyl-alpha-D-galactopyranoside + D-glucose
sucrose + D-glucose
glucose + sucrose
sucrose + D-xylose
beta-D-fructofuranosyl-alpha-D-xylopyranoside + D-glucose
sucrose + D-xylose
beta-D-fructofuranosyl-beta-D-xylopyranoside + D-glucose
-
-
-
?
sucrose + D-xylose
D-glucose + alpha-D-xylopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
-
NMR product analysis
-
?
sucrose + fructose
?
-
-
-
?
sucrose + galactose
Fru(beta-2,6)Gal + ?
sucrose + glucose
1-kestotriose + 6-kestotriose + 1-kestotetraose + ?
sucrose + glucose
?
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
sucrose + H2O
glucose + fructose + levan + sucrose + fructooligosaccharides
-
-
-
-
?
sucrose + hydroquinone
D-glucose + 4-hydroxyphenyl-beta-D-fructofuranoside
sucrose + inositol
?
-
low activity
-
-
?
sucrose + inulin
?
-
-
-
-
?
sucrose + isomaltose
D-glucose + theanderose
sucrose + isomaltose
isomaltosyl-fructose + D-glucose
-
-
-
?
sucrose + L-arabinose
6-kestotriose + ?
-
-
-
?
sucrose + L-arabinose
6-kestotriose + fructosylated L-arabinose
-
-
-
?
sucrose + L-arabinose
?
-
-
-
-
?
sucrose + L-galactose
beta-D-fructofuranosyl-beta-L-galactopyranoside + D-glucose
-
-
-
?
sucrose + L-glucose
beta-D-fructofuranosyl-beta-L-glucopyranoside + D-glucose
-
-
-
?
sucrose + L-xylose
beta-D-fructofuranosyl-beta-L-xylopyranoside + D-glucose
-
-
-
?
sucrose + lactose
beta-D-galactopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
-
-
-
?
sucrose + lactose
D-glucose + beta-D-fructofuranosyl-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside
-
-
-
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + lactose
D-glucose + lactosylfructoside
sucrose + lactose
D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
sucrose + lactose
fructosylated lactose + ?
-
-
-
?
sucrose + lactose
lactosucrose
sucrose + lactose
lactosucrose + D-glucose
the production condition is optimized as pH at 6.0, temperature at 35°C, 5 units/ml enzyme, 180 g/l sucrose, and 180 g/l lactose
-
-
?
sucrose + levan
?
mutant enzymes H243L and S164A synthesize either high or low levan molecular weight
-
-
?
sucrose + maltose
D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + maltose
erlose + D-glucose
-
-
-
?
sucrose + maltotriose
?
-
-
-
-
?
sucrose + mannose
?
-
-
-
?
sucrose + melibiose
?
-
-
-
-
?
sucrose + melibiose
D-glucose + raffinose
sucrose + melibiose
raffinose + D-glucose
sucrose + methanol
glucose + methyl-beta-D-fructoside
sucrose + polygalacturonic acid
?
-
-
-
?
sucrose + raffinose
Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ?
-
-
-
?
sucrose + raffinose
melibiose + ?
-
-
-
-
?
sucrose + raffinose
melibiose + D-fructose + levan
-
-
-
?
sucrose + stachyose
manninotriose + D-fructose
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
sucrose + sucrose
6-kestose + levanbiose + blastose + 1-kestose + ?
sucrose + sucrose
D-glucose + (2,6-beta-D-fructosyl)3
Q60114
dimer form
degree of polymerisation 3
-
?
sucrose + sucrose
D-glucose + 1-kestose
sucrose + sucrose
D-glucose + 1-kestose + nystose + ?
sucrose + sucrose
D-glucose + 6-kestose
sucrose + sucrose
D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
sucrose + sucrose
D-glucose + levan
sucrose + sucrose
D-glucose + levan + fructooligosaccharides
-
-
-
?
sucrose + sucrose
D-glucose + levan type-fructan
sucrose + sucrose
D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)
sucrose + sucrose
D-glucose + neokestose
-
-
identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)
-
?
sucrose + sucrose
D-glucose + nystose
-
-
identified by HPAEC
-
?
sucrose + sucrose
kestose + D-glucose
-
high concentration of sucrose
-
-
?
sucrose + triisopropylbenzenesulfonyllevan
?
-
-
-
-
?
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Q60114
pH lower than 7.0, fibril form
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
surgarcane molasse
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
sweet sorghum juice
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
table sugar
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
additional information
?
-
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
-
-
-
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
-
-
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
-
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
-
-
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
-
only one mannosyloligofructoside is formed
-
-
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
-
only one mannosyloligofructoside is formed
-
-
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
-
-
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
-
-
?
inulin + H2O
?
-
-
-
-
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
-
the levan synthesized on raffinose contains one mol of galactosylglucose per mol as one of the 2 terminal glycosyl moieties
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
in 0.38 M phosphate buffer
-
-
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose
levan + fructooligosaccharides
the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
-
-
?
sucrose
levan + fructooligosaccharides
the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
-
-
?
sucrose
levan + kestose
-
at concentrations below 85 mM (at 37°C). Above 85 mM sucrose, the transglycosylation activity of Lev gradually becomes significant. Transglycosylation never exceeds 50% of total enzyme activity
-
-
?
sucrose
levan + kestose
-
at concentrations below 85 mM (at 37°C). Above 85 mM sucrose, the transglycosylation activity of Lev gradually becomes significant. Transglycosylation never exceeds 50% of total enzyme activity
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
reduction of glucose by yeasts (Candida cacaoi DSM 2226), introduced into a dialysing membrane, situated in the reaction medium, and the presence of Mn2+ results in an increase of levan synthesis efficiency to 64%
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
reverse reaction is probably catalyzed by a different levanase
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
reverse reaction is probably catalyzed by a different levanase
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
high molecular weight branched levan, product forms a complex with the enzyme
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
Aerobacter levanicum
-
-
product forms a complex with the enzyme
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
Aerobacter levanicum
-
levan biosynthesis
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
activity is affected by sacU mutation
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
polymerase and hydrolase activity can be separately modulated by site-directed mutagenesis
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
reaction kinetics are dependent on sucrose concentration
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
activity is affected by sacU mutation
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
activity is affected by sacU mutation
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
various acceptors
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
beta-D-fructofuranosidase activity
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
synthesis of a high-molecular-mass fructan of the levan type
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
translocation of the enzyme is a rate-limiting step
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
additional beta-fructosidase, i.e. invertase, sucrose-sucrose 1-fructosyltransferase, and sucrose-sucrose 6-fructosyltransferase activities of recombinant enzyme from Pichia pastoris
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
various acceptors, e.g. fructosyl acceptors 2-1 linked or 2-6 linked, including water, the latter belongs to a fructosidase, i.e. invertase, activity of the purified enzyme, overview
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
transgenic plants
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
transgenic plants
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
key enzyme for the formation of the graminans and phleins
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
hydrolase, i.e. invertase, and transferase activities can be altered by modifying reaction conditions
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
beta-D-fructofuranosidase activity
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
molecular weight of levan product is dependent on temperature, salinity, and sucrose concentration
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
various D-fructosyl acceptors, overview
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
transfucosylation from sucrose to various acceptors, except sugar alcohols
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
transfucosylation from sucrose to various acceptors, except sugar alcohols
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
the degree of polymerization of levan produced at high temperature is lower than that produced at low temperature
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
various D-fructosyl acceptors, overview
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
Q60114
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
-
enzyme plays an important role in the production of ethanol from sugar cane and other sucrose sources
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
Q60114
-
molecular weight of produced levan is dependent on preparation method, immobilized recombinant enzyme and recombinant enzyme in permeabilized E. coli cells produces low molecular weight levan, free recombinant enzyme produces high molecular weight levan
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
at lower temperatures, such as 5°C and 15°C and pH 4.0-6.0 the transfructosylation is preferentially catalyzed rather than the hydrolysis of sucrose, but inversely at higher temperatures such as 30°C and 40°C and pH 7.0-8.0 the hydrolysis is preferentially catalyzed
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
enzyme catalyzes the liberation of reducing sugars from substrates having 2-beta-D-fructofuranose residues on a terminal such as sucrose, raffinose, levan from Aerobacter levanicum and inulin with a relative activity of 100:104:1:0.01
-
r
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
Q60114
-
-
?
sucrose + ?
blastose + ?
-
-
two-dimensional COSY, TOCSY, HMBC and HSQC experiments
-
?
sucrose + ?
blastose + ?
-
-
two-dimensional COSY, TOCSY, HMBC and HSQC experiments
-
?
sucrose + ?
polyfructan + ?
-
-
polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages
-
?
sucrose + ?
polyfructan + ?
-
-
polyfructan, molecular mass of 2711 kDa and consisted mainly of beta(2-6) linkages
-
?
sucrose + acarbose
D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose
-
-
product analysis and structure determination by NMR spectroscopy
-
?
sucrose + acarbose
D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose
-
-
product analysis and structure determination by NMR spectroscopy
-
?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
-
-
-
?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
-
-
-
?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
-
-
?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
-
-
?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
-
the highest product molecular weight of 38 kDa is reached at 30°C, and gradually decreases at higher temperatures
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
sucrose is used as a starter unit to which fructose residues, cleaved from another sucrose molecule, become attached
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
sucrose is used as a starter unit to which fructose residues, cleaved from another sucrose molecule, become attached
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + cellobiose
beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
-
-
-
?
sucrose + cellobiose
beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
-
-
-
?
sucrose + cellobiose
D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
the major transfructosylation product is identified to be cellobiose-fructose (beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside)
-
?
sucrose + cellobiose
D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + cellobiose
D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + cellobiose
fructosylated cellobiose + ?
-
-
-
?
sucrose + cellobiose
fructosylated cellobiose + ?
-
-
-
?
sucrose + D-fucose
beta-D-fructofuranosyl-alpha-D-fucopyranoside + D-glucose
-
-
-
?
sucrose + D-fucose
beta-D-fructofuranosyl-alpha-D-fucopyranoside + D-glucose
-
-
-
?
sucrose + D-galactose
beta-D-fructofuranosyl-alpha-D-galactopyranoside + D-glucose
-
-
-
?
sucrose + D-galactose
beta-D-fructofuranosyl-alpha-D-galactopyranoside + D-glucose
-
-
-
?
sucrose + D-glucose
glucose + sucrose
-
exchange reaction
-
?
sucrose + D-glucose
glucose + sucrose
-
exchange reaction
-
?
sucrose + D-maltose
?
-
-
-
?
sucrose + D-maltose
?
-
-
-
-
?
sucrose + D-maltose
?
-
-
-
-
?
sucrose + D-maltose
?
-
-
-
-
?
sucrose + D-sorbitol
?
-
low activity
-
-
?
sucrose + D-sorbitol
?
-
-
-
-
?
sucrose + D-sorbitol
?
-
-
-
-
?
sucrose + D-xylose
?
-
-
-
?
sucrose + D-xylose
?
-
-
-
?
sucrose + D-xylose
?
-
-
-
-
?
sucrose + D-xylose
?
-
-
-
-
?
sucrose + D-xylose
beta-D-fructofuranosyl-alpha-D-xylopyranoside + D-glucose
-
-
-
?
sucrose + D-xylose
beta-D-fructofuranosyl-alpha-D-xylopyranoside + D-glucose
-
-
-
?
sucrose + D-xylose
D-glucose + alpha-D-xylopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + D-xylose
D-glucose + alpha-D-xylopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + galactose
Fru(beta-2,6)Gal + ?
-
-
-
?
sucrose + galactose
Fru(beta-2,6)Gal + ?
-
-
-
?
sucrose + glucose
1-kestotriose + 6-kestotriose + 1-kestotetraose + ?
-
-
-
?
sucrose + glucose
1-kestotriose + 6-kestotriose + 1-kestotetraose + ?
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
hydrolysis
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
-
hydrolysis
-
-
?
sucrose + hydroquinone
D-glucose + 4-hydroxyphenyl-beta-D-fructofuranoside
-
-
NMR product analysis, the product shows anti-oxidation activities and inhibition of tyrosinase and of 1,1-diphenyl-2-picrylhydrazyl scavenging activity, it also shows inhibition of lipid peroxidation, kinetics, overview
-
?
sucrose + hydroquinone
D-glucose + 4-hydroxyphenyl-beta-D-fructofuranoside
-
-
NMR product analysis, the product shows anti-oxidation activities and inhibition of tyrosinase and of 1,1-diphenyl-2-picrylhydrazyl scavenging activity, it also shows inhibition of lipid peroxidation, kinetics, overview
-
?
sucrose + isomaltose
D-glucose + theanderose
-
-
-
-
?
sucrose + isomaltose
D-glucose + theanderose
-
-
-
-
?
sucrose + isomaltose
D-glucose + theanderose
-
-
-
-
?
sucrose + lactose
?
-
-
-
?
sucrose + lactose
?
-
-
-
?
sucrose + lactose
?
-
-
-
-
?
sucrose + lactose
?
-
-
-
-
?
sucrose + lactose
?
-
-
-
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
the major transfructosylation product is identified to be lactosefructose or lactosucrose (beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside)
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme
i.e. lactosefructose or lactosucrose
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
optimized conditions are pH 6.5, 50°C, 27% w/v sucrose, 27% w/v lactose, and 5 U/ml of the purified recombinant enzyme
i.e. lactosefructose or lactosucrose
-
?
sucrose + lactose
D-glucose + lactosylfructoside
-
method optimization for lactosylfructoside biosynthesis, overview. Equal concentration of the two substrates showed a better performance than the other concentration ratios
lactosylfructoside is a functional oligosaccharide consisting of D-glucose, D-galactose, and D-fructose. Purification of the trisaccharide from the product using a HPLC system and confirmation by NMR spectroscopy
-
?
sucrose + lactose
D-glucose + lactosylfructoside
-
method optimization for lactosylfructoside biosynthesis, overview. Equal concentration of the two substrates showed a better performance than the other concentration ratios
lactosylfructoside is a functional oligosaccharide consisting of D-glucose, D-galactose, and D-fructose. Purification of the trisaccharide from the product using a HPLC system and confirmation by NMR spectroscopy
-
?
sucrose + lactose
D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose
i.e. lactosucrose or 4G-beta-D-galactosylsucrose
-
?
sucrose + lactose
D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
levansucrase transfers the fructosyl moiety of sucrose to lactose to form lactosucrose, optimization of reaction conditions, 28.5% conversion in absence and 43.2% in presence of glucose oxidase, which eliminates the inhibitory D-glucose reaction product, overview
i.e. lactosucrose or 4G-beta-D-galactosylsucrose, product identification and quantification by NMR spectroscopy
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
Bacillus subtilis is the most effective producer of lactofructose
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + lactose
lactosucrose
-
-
-
-
?
sucrose + maltose
?
-
-
-
?
sucrose + maltose
?
-
-
-
?
sucrose + maltose
?
-
-
-
-
?
sucrose + maltose
D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + maltose
D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
-
-
-
-
?
sucrose + melibiose
D-glucose + raffinose
-
-
-
?
sucrose + melibiose
D-glucose + raffinose
-
when 30% (w/v) sucrose and 30% (w/v) melibiose are catalyzed using 3% (w/v) whole cells at pH 6.5 (50 mM sodium phosphate buffer) and 55°C, the highest raffinose yield is 222 g/l after a 6 h reaction. The conversion ratio from each substrate to raffinose is 50%
-
-
?
sucrose + melibiose
D-glucose + raffinose
-
when 30% (w/v) sucrose and 30% (w/v) melibiose are catalyzed using 3% (w/v) whole cells at pH 6.5 (50 mM sodium phosphate buffer) and 55°C, the highest raffinose yield is 222 g/l after a 6 h reaction. The conversion ratio from each substrate to raffinose is 50%
-
-
?
sucrose + melibiose
raffinose + D-glucose
-
-
-
?
sucrose + melibiose
raffinose + D-glucose
-
-
-
?
sucrose + methanol
glucose + methyl-beta-D-fructoside
-
-
-
-
?
sucrose + methanol
glucose + methyl-beta-D-fructoside
-
-
-
?
sucrose + sorbitol
?
-
-
-
?
sucrose + sorbitol
?
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
-
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
-
-
-
-
?
sucrose + sucrose
6-kestose + levanbiose + blastose + 1-kestose + ?
-
-
-
-
?
sucrose + sucrose
6-kestose + levanbiose + blastose + 1-kestose + ?
-
-
-
-
?
sucrose + sucrose
?
-
-
-
-
?
sucrose + sucrose
?
-
product concentrations of fructose, kestose or fructan depend on the concentration of sucrose, pH and temperatur
-
-
?
sucrose + sucrose
D-glucose + 1-kestose
-
-
identified by HPAEC
-
?
sucrose + sucrose
D-glucose + 1-kestose
-
-
identified by HPAEC
-
?
sucrose + sucrose
D-glucose + 1-kestose + nystose + ?
-
-
-
-
?
sucrose + sucrose
D-glucose + 1-kestose + nystose + ?
-
-
-
-
?
sucrose + sucrose
D-glucose + 6-kestose
-
-
identified by HPAEC
-
?
sucrose + sucrose
D-glucose + 6-kestose
-
-
identified by HPAEC
-
?
sucrose + sucrose
D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
-
-
-
-
?
sucrose + sucrose
D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
-
-
-
?
sucrose + sucrose
D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
the enzyme has high product specificity, and no fructooligosaccharide is identified in the product
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
the recombinant enzyme shows 80% conversion yield
-
-
?
sucrose + sucrose
D-glucose + levan
the recombinant enzyme shows 80% conversion yield
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
-
-
?
sucrose + sucrose
D-glucose + levan
-
is assayed in a standard reaction containing 1 ml 10% sucrose and 1 ml enzyme (0.01-0.05mg/ml) incubated at 15°C
-
-
?
sucrose + sucrose
D-glucose + levan type-fructan
-
-
-
?
sucrose + sucrose
D-glucose + levan type-fructan
-
-
-
?
sucrose + sucrose
D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)
-
-
-
?
sucrose + sucrose
D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
additional information
?
-
Aerobacter levanicum
-
6-beta-D-fructosyl)n is levan
-
-
?
additional information
?
-
design and evaluation of a bi-enzymatic system: combined use of levansucrase from Bacillus amyloliquefaciens and endoinulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides (FOS) and oligolevans using sucrose as the sole substrate, overview. At the initial stage of time course, short chain fructooligosaccharides, 1-kestose, nystose, and 1F-fructosylnystose are the major products, whilst 6-kestose, medium chain fructooligosaccharides (mcFOSs, levanohexaose, levanopentaose) and oligolevans become the dominant ones at the late stage. NMR analysis of levan, levanopentaose and levanohexaose, overview
-
-
?
additional information
?
-
levansucrase catalyzes simultaneously both the transfructosylation and the hydrolysis, which is regarded as the transfer of the fructosyl group to water, time courses for transfructosylation and hydrolysis reactions, overview. Measurement of sucrose hydrolysis releasing D-glucose and D-fructose. Disaccharides lactose, cellobiose and melibiose are used as acceptors, with high acceptor specificity towards lactose. Maple syrup is used as assay medium and sucrose source, profile of the end-products synthesized by the enzyme, overview
-
-
?
additional information
?
-
-
levansucrase catalyzes simultaneously both the transfructosylation and the hydrolysis, which is regarded as the transfer of the fructosyl group to water, time courses for transfructosylation and hydrolysis reactions, overview. Measurement of sucrose hydrolysis releasing D-glucose and D-fructose. Disaccharides lactose, cellobiose and melibiose are used as acceptors, with high acceptor specificity towards lactose. Maple syrup is used as assay medium and sucrose source, profile of the end-products synthesized by the enzyme, overview
-
-
?
additional information
?
-
design and evaluation of a bi-enzymatic system: combined use of levansucrase from Bacillus amyloliquefaciens and endoinulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides (FOS) and oligolevans using sucrose as the sole substrate, overview. At the initial stage of time course, short chain fructooligosaccharides, 1-kestose, nystose, and 1F-fructosylnystose are the major products, whilst 6-kestose, medium chain fructooligosaccharides (mcFOSs, levanohexaose, levanopentaose) and oligolevans become the dominant ones at the late stage. NMR analysis of levan, levanopentaose and levanohexaose, overview
-
-
?
additional information
?
-
-
levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule, including polymerization, transfructosylation, and hydrolysis. Wide substrate specificity of levansucrase toward monosaccharides, disaccharides, and aromatic and alkyl alcohols, producing diverse sucrose analogues, hetero-oligosaccharides (especially lactosucrose), and fructosides, overview
-
-
?
additional information
?
-
-
broad transglycosylation capability of the levansucrase from Bacillus licheniformis strain 8-37-0-1, substrate specificity, overview. When using sucrose as the donor, the enzyme displays a wide range of acceptor specificity and is able to transfer fructosyl to a series of sugar acceptors including hexose, pentose, beta- or alpha-disaccharides, along with the difficult branched alcohols. Product analysis by mass and NMR spectrometry
-
-
?
additional information
?
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule: polymerization (using the growing fructan chain as an acceptor), transfructosylation (using monosaccharides, disaccharides, or oligosaccharides as acceptors), and hydrolysis (using water as an acceptor), overview
-
-
?
additional information
?
-
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
-
broad transglycosylation capability of the levansucrase from Bacillus licheniformis strain 8-37-0-1, substrate specificity, overview. When using sucrose as the donor, the enzyme displays a wide range of acceptor specificity and is able to transfer fructosyl to a series of sugar acceptors including hexose, pentose, beta- or alpha-disaccharides, along with the difficult branched alcohols. Product analysis by mass and NMR spectrometry
-
-
?
additional information
?
-
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
mutant enzymes Y429N and R433A no longer produce levan but exclusively oligosaccharides
-
-
?
additional information
?
-
-
mutant enzymes Y429N and R433A no longer produce levan but exclusively oligosaccharides
-
-
?
additional information
?
-
-
levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules included in the reaction medium
-
-
?
additional information
?
-
-
levansucrase catalyzes the synthesis of levan from sucrose, but it may also transfer the fructosyl moiety from sucrose to acceptor molecules. The wild-type enzyme synthesizes levan with molecular weight bimodal distribution, while the mutants R360K and Y429N synthesize only oligosaccharides, substrate specificities, overview
-
-
?
additional information
?
-
levan synthesis from sucrose, structure analysis by NMR spectrometry, overview. Levan size analysis by gel filtration. As sucrose concentration increases, the average molecular weight of the low dextran distribution decreases, at 4°C compared to 37°C, the low molecular weight distribution region increases in size at any given substrate concentration, and a maximum of 20.64 kDa is obtained at 200 g/l
-
-
?
additional information
?
-
-
levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule, including polymerization, transfructosylation, and hydrolysis. Wide substrate specificity of levansucrase toward monosaccharides, disaccharides, and aromatic and alkyl alcohols, producing diverse sucrose analogues, hetero-oligosaccharides (especially lactosucrose), and fructosides, overview
-
-
?
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule: polymerization (using the growing fructan chain as an acceptor), transfructosylation (using monosaccharides, disaccharides, or oligosaccharides as acceptors), and hydrolysis (using water as an acceptor), overview
-
-
?
additional information
?
-
measurement of sucrose hydrolysis, releasing D-glucose and D-fructose. As the reaction proceeds, SacB becomes less hydrolytic while transferase becomes preponderant to reach up to 80% at the end of the reaction
-
-
?
additional information
?
-
levan synthesis from sucrose, structure analysis by NMR spectrometry, overview. Levan size analysis by gel filtration. As sucrose concentration increases, the average molecular weight of the low dextran distribution decreases, at 4°C compared to 37°C, the low molecular weight distribution region increases in size at any given substrate concentration, and a maximum of 20.64 kDa is obtained at 200 g/l
-
-
?
additional information
?
-
measurement of sucrose hydrolysis, releasing D-glucose and D-fructose. As the reaction proceeds, SacB becomes less hydrolytic while transferase becomes preponderant to reach up to 80% at the end of the reaction
-
-
?
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
in Lactobacillus sanfranciscensis TMW 1.392, sucrose metabolism and formation of fructan and 1-kestose is dependent on the activity of a single enzyme, levansucrase
-
-
?
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
in Lactobacillus sanfranciscensis TMW 1.392, sucrose metabolism and formation of fructan and 1-kestose is dependent on the activity of a single enzyme, levansucrase
-
-
?
additional information
?
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory
-
-
-
additional information
?
-
-
the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory
-
-
-
additional information
?
-
the enzyme cannot hydrolyze levan from Halomonas smyrnensis, Zymomonas mobilis and Bacillus subtilis, and inulin from chicory
-
-
-
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levan synthesis from sucrose
-
-
?
additional information
?
-
-
measurement of sucrose hydrolysis
-
-
?
additional information
?
-
-
levan synthesis from sucrose
-
-
?
additional information
?
-
-
measurement of sucrose hydrolysis
-
-
?
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule, including polymerization, transfructosylation, and hydrolysis. Wide substrate specificity of levansucrase toward monosaccharides, disaccharides, and aromatic and alkyl alcohols, producing diverse sucrose analogues, hetero-oligosaccharides (especially lactosucrose), and fructosides, overview
-
-
?
additional information
?
-
-
levansucrase catalyzes three distinct reactions depending on the fructosyl acceptor molecule: polymerization (using the growing fructan chain as an acceptor), transfructosylation (using monosaccharides, disaccharides, or oligosaccharides as acceptors), and hydrolysis (using water as an acceptor), overview
-
-
?
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
-
levan, a polymer of fructose linked by fructofurano side bonds, is produced by the transfructosylation reaction of levansucrase
-
-
?
additional information
?
-
the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
-
-
-
additional information
?
-
the enzyme also shows hydrolytic activity with sucrose producing D-glucose and D-fructose
-
-
-
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
-
-
?
additional information
?
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions. The enzyme cleaves raffinose and stachyose
-
-
?
additional information
?
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the product levan has not a major impact on biofilm formation
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additional information
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thermo-responsive expression and differential secretion of the enzyme. The extracellular levansucrase is encoded by two functional genes, lscB and lscC. Transcription of lscB and lscC is temperature-dependent. Quantification of Lsc in supernatants and cellular protein samples of mutants defective in either lscB or lscC confirm that LscB secretion at low temperature is due to a combination of thermo-regulated transcription and secretion. LscC accumulates in the periplasmic space
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levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions. No activity with raffinose or stachyose
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additional information
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isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview
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additional information
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assay of the growth phenotype of levansucrase-expressing bacteria on agar plate containing sucrose. Only wild-type Lsc3 produces levan giving a mucoid phenotype to the streaks of respective transformant. Quantitative evaluation of the sucrose-splitting (total) levansucrase activity on microplates
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additional information
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assay of the growth phenotype of levansucrase-expressing bacteria on agar plate containing sucrose. Only wild-type Lsc3 produces levan giving a mucoid phenotype to the streaks of respective transformant. Quantitative evaluation of the sucrose-splitting (total) levansucrase activity on microplates
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additional information
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levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions. The enzyme cleaves raffinose and stachyose
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additional information
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levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions. The enzyme cleaves raffinose and stachyose
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additional information
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levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions. The enzyme cleaves raffinose and stachyose
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additional information
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levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions. The enzyme cleaves raffinose and stachyose
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additional information
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isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview
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additional information
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assay of the growth phenotype of levansucrase-expressing bacteria on agar plate containing sucrose. Only wild-type Lsc3 produces levan giving a mucoid phenotype to the streaks of respective transformant. Quantitative evaluation of the sucrose-splitting (total) levansucrase activity on microplates
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additional information
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the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
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additional information
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the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
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additional information
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the enzyme also hydrolyzes sucrose to D-glucose and D-fructose
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Q60114
production of low molecular weight levan in permeabilized recombinant E. coli cells
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6-beta-D-fructosyl)n is levan
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additional information
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Q60114
change in substrate specificity appears to be unique to Zymomonas mobilis levansucrase because the levansucrases from Bacillus subtilis, Rahnella aquatilis, and Lactobacillus reuteri are capable of synthesizing levan in their soluble form and are not reported to form fibril-like structures
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additional information
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change in substrate specificity appears to be unique to Zymomonas mobilis levansucrase because the levansucrases from Bacillus subtilis, Rahnella aquatilis, and Lactobacillus reuteri are capable of synthesizing levan in their soluble form and are not reported to form fibril-like structures
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additional information
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levan biosynthesis and fructooligosaccharide biosynthesis
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additional information
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levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
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additional information
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three activities are displayed by the levansucrase from Zymomonas mobilis: formation of levan (polymerization), production of short-chain fructooligosaccharides (FOS), and sucrose hydrolysis. Several inulin-type FOS (1-kestose, nystose, 1F-fructosylnystose), neo-FOS (blastose, neokestose, neonystose) and levan-type FOS (6-kestose, 6,6-nystose) are synthesized by levansucrase, identification by mass spectroscopy and NMR spectrometry, e.g. of 6,6-nystose (i.e. Fru-beta(2->6)-Fru-beta(2->6)-Fru-beta(2->1)-alphaGlc). Assay method optimization, transfructosylation to hydrolysis ratio. , overview
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additional information
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levan biosynthesis and fructooligosaccharide biosynthesis
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