2.1.1.35: tRNA (uracil54-C5)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (uracil54-C5)-methyltransferase, go to the full flat file.

Reaction

Synonyms

5-methyluridine 54 tRNA methyltransferase, m5U 54 tRNA methyltransferase, m5U 54 tRNA MTase, m5U methyltransferase, m5U-methyltransferase, methyltransferase, transfer ribonucleate uracil 5-, PAB0719, PAB0719 enzyme, PabTrmU54, ribothymidyl synthase, rTase, RumA, RumA-type tRNA(uracil-54, C5)-methyltransferase, RUMT, S-adenosyl-L-methionine-dependent tRNA (uracil-54, C5)-methyltransferase, transfer RNA uracil 5-methyltransferase, transfer RNA uracil methylase, TrmA, TRMT2A, tRNA (uracil-54, C5)-methyltransferase, tRNA uracil 5-methyltransferase, tRNA(m5U54)methyltransferase, tRNA(uracil-5-)methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.35 tRNA (uracil54-C5)-methyltransferase

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Results	in table
9	Activating Compound
8744	AA Sequence
3	Application
1	CAS Registry Number
12	Cloned(Commentary)
30	Cofactor
2	Crystallization (Commentary)
19	Disease/ Diagnostics
1	General Information
2	General Stability
42	Inhibitors
4	Ki Value [mM]
23	KM Value [mM]
9	Localization
12	Metals/Ions
13	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
102	Organism
2	PDB ID
10	pH Optimum
1	pH Range
13	Protein Variants
18	Purification (Commentary)
16	Reaction
3	Reaction Type
45	Reference
2	Source Tissue
8	Specific Activity [micromol/min/mg]
6	Storage Stability
152	Substrates and Products (Substrate)
12	Subunits
26	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
1	Temperature Stability [°C]
10	Turnover Number [1/s]

Cloned

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Cloned on EC 2.1.1.35 - tRNA (uracil54-C5)-methyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloning and sequencing of the TRM2 gene encoding enzyme, gene is previously identified as RNC1/NUD1 gene, but does not encode the yNucR endo-exonuclease, TRM2 gene is not essential for cell viability and contains alternative translational start sites, glutathione S-transferase tagged recombinant, Trm2p, is expressed in Escherichia coli

Saccharomyces cerevisiae

-

441380

enzyme is encoded by the nonessential TRM2 gene

Saccharomyces cerevisiae

-

441381

expressed in Escherichia coli as a His-tagged fusion protein

Pyrococcus abyssi

-

721031

genes PAB0719 and PAB0760, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)

Pyrococcus abyssi

-

705768

hybrid plasmid carrying trmA gene encoding enzyme is used to amplify enzyme production, trmA gene is sequenced

Escherichia coli

-

441365

into the pET28b expression vector

Escherichia coli

P23003

689809

the plasmids pGP100, pDM4, pKD4 and pKD46 are used for the construction of the mutants

Escherichia coli

-

689248

trmA gene encodes enzyme, gene is essential for the organism

Escherichia coli

-

441361, 441372, 441376

trmA gene encoding for RUMT is cloned into a pET vector and transformed into Escherichia coli BL21 (DE3) cells, extremely high-level expression system

Escherichia coli

-

441373

trmA gene is cloned under control of the inducible lac promoter, DH5alpha

Escherichia coli

-

441378