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2.1.1.11: magnesium protoporphyrin IX methyltransferase

This is an abbreviated version!
For detailed information about magnesium protoporphyrin IX methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.11

Reaction

S-adenosyl-L-methionine
+
magnesium protoporphyrin IX
=
S-adenosyl-L-homocysteine
 +
magnesium protoporphyrin IX 13-methyl ester

Synonyms

(-)-S-adenosyl-L-methionine:magnesium-protoporphyrin IX methyltransferase, BchM, ChlM, LOC_Os06g04150, magnesium protoporphyrin IX methyltransferase, magnesium protoporphyrin methyltransferase, magnesium protoporphyrin O-methyltransferase, methyltransferase, magnesium protoporphyrin, Mg-protoporphyrin IX methyltransferase, MgPIXMT protein, MgPMT, S-adenosyl-L-methionine:Mg protoporphyrin methyltransferase, Ygl18

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.11 magnesium protoporphyrin IX methyltransferase

Cloned

Cloned on EC 2.1.1.11 - magnesium protoporphyrin IX methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a cDNA library is constructed
derived from plasmid pRPS404, consisting of a 46 kb pair section of the photosynthetic gene cluster, overexpression of His6-tagged protein in Escherichia coli, strain BL21DE3, expression plasmid pHisBchM
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli JM109, constitutive promoter on pUCmod, overexpression improved by using BL21 cells in combination with T7 promoter, subclconing into pET-20b(+) vector, engineered Escherichia coli strain overproducing protoporphyrin for activity assays
-
expression in Escherichia coli, pCambia derivative, binary vector introduced into the GV3121 strain of Agrobacterium tumefaciens, transgenic plants generated, EYFP fusion protein for localization studies, insertion of the T-DNA in single intron present in the coding sequence, deletion of the 5' part of the gene including the entire first exon, null-mutant
-
for sequence determination, mutant complementation
-
into the vector pET9a for expression in Escherichia coli BL21DE3 cells
-
overexpression in Escherichia coli