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1.97.1.12: photosystem I

This is an abbreviated version!
For detailed information about photosystem I, go to the full flat file.

Word Map on EC 1.97.1.12

Reaction

reduced plastocyanin
+
oxidized ferredoxin
+
hnu
=
oxidized plastocyanin
+
reduced ferredoxin

Synonyms

OsCRR6, photosystem I P700 chlorophyll a apoprotein A2, PS I, PS-I, PS-I complex, PsaB, PsaF, PSI, PSI core complex

ECTree

     1 Oxidoreductases
         1.97 Other oxidoreductases
             1.97.1 Sole sub-subclass for oxidoreductases that do not belong in the other subclasses
                1.97.1.12 photosystem I

Purification

Purification on EC 1.97.1.12 - photosystem I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from thyloakoid membranes, by detergent solubilization
native enzyme complex from detergent-treated thylakoid membranes by two steps of anion exchange chromatography with elution through tetraethylammonium chloride followed by precipitation with PEG 6000 and PEG 1500, respectively
PsaE protein is purified to homogeneity by affinity chromatography. Subunit PsaE (a peripheral subunit of the PSI complex)is involved in the docking of ferredoxin/flavodoxin to the PSI complex and also participates in the cyclic electron transfer around phosphosystem I
-
purification of the luminal domain of spinach photosystem 1 subunit PsaF
-
rapid, high-yield purification of PS1
-
recombinant His6-tagged enzyme from thylakoid membranes by nickel affinity chromatography and ultrafiltration