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1.97.1.12: photosystem I

This is an abbreviated version!
For detailed information about photosystem I, go to the full flat file.

Word Map on EC 1.97.1.12

Reaction

reduced plastocyanin
+
oxidized ferredoxin
+
hnu
=
oxidized plastocyanin
+
reduced ferredoxin

Synonyms

OsCRR6, photosystem I P700 chlorophyll a apoprotein A2, PS I, PS-I, PS-I complex, PsaB, PsaF, PSI, PSI core complex

ECTree

     1 Oxidoreductases
         1.97 Other oxidoreductases
             1.97.1 Sole sub-subclass for oxidoreductases that do not belong in the other subclasses
                1.97.1.12 photosystem I

Crystallization

Crystallization on EC 1.97.1.12 - photosystem I

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3.3 A crystal structure of the entire PSI super-complex, sitting drop variant of the vapor-diffusion technique at 4°C
-
purified plant photosystem I supercomplex including light-harvesting complex I, sitting drop vapour diffusion technique, mixing of 0.006-0.008 ml of PSI protein solution with an equal volume of reservoir solution containing 50 mM di-potassium phosphate, 50 mM Tris, pH 8, 12-17% PEG 400, 1% glycerol, 2 mM L-glutathione, and 0.03% octyl glucose neopentyl glycol, and equilibration against 0.5 ml of reservoir solution, 4°C, 1 month, X-ray diffraction structure determination and analysis at 2.6 A resolution, single-wavelength anomalous diffraction phasing of PSI-LHCI
sitting-drop variant of the vapour-diffusion technique at 4°C, crystals diffract to 4 A resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P21, with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 A, beta = 90.484
-
single crystals from a complex of photosystem I with ferredoxin are grown using PEG 400 and CaCl2 as precipitation agents. The crystals diffract X-rays to a resolution of 7–8 A. The space group iss determined to be orthorhombic with the unit cell dimensions a = 194 A, b = 208 A, and c = 354 A. The crystals contain photosystem I and ferredoxin in a 1:1 ratio. Electron paramagnetic resonance (EPR) measurements on these crystals are reported, where EPR signals of the three [4Fe-4S] clusters FA, FB, FX, and the [2Fe-2S] cluster of ferredoxin are detected
-
highly ordered two-dimensional crystals of photosystem I reaction center complex
-
isolation and reconstitution of the functional PSI complexes into 2D arrays. The solubilized and crystallized PSI has essentially the same protein composition, as shown by SDS/polyacrylamide gels
-