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1.8.3.1: sulfite oxidase

This is an abbreviated version!
For detailed information about sulfite oxidase, go to the full flat file.

Word Map on EC 1.8.3.1

Reaction

sulfite
+
O2
+
H2O
=
sulfate
+
H2O2

Synonyms

At-SO, AtSOX, CG7280, HSO, NIA, oxidase, sulfite, PSO, Shopper, SO, SorT, SOX, sulfite oxidase, sulfite oxidase homologue, sulfite: acceptor oxidoreductase, sulfite:acceptor oxidoreductase, sulfite:oxygen oxidoreductase, sulphite oxidase cytochrome b9, SUOX, YedY, YedYZ, ZmSO

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.3 With oxygen as acceptor
                1.8.3.1 sulfite oxidase

Purification

Purification on EC 1.8.3.1 - sulfite oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
70-77% pure
-
75% pure
-
acetone fractionation, ion exchange chromatography and Sephadex gel filtration
-
by cation-exchange adsorber and ultra filtration, 20fold purified
-
DEAE 52 column chromatography, Superdex X26 gel filtration and hydroxyapatite column chromatography
-
glutathione-Sepharose resin column chromatography and Superdex 200 gel filtration
His-Select HF nickel affinity gel column chromatography
-
His-tagged enzyme expressed in Escheria coli
-
isolation of heme-and molybdenum-containing fragments
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Mono-Q column chromography
-
nickel affinity chromatography
-
nickelnitrilotriacetic acid superflow matrix followed by anion exchange chromatography on a SourceQ15 column
-
on Ni-NTA resin
-
partial
phenyl-Sepharose column chromatography and Superdex 200 16/60 FPLC column gel filtration
-
recombinant protein from Escherichia coli
-
recombinant R160Q
-
recombinant wild-type and mutant enzymes
Superdex 200 FPLC column gel filtration
-
wild-type and mutant Y343F
-